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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NGEF All Species: 30
Human Site: T504 Identified Species: 66
UniProt: Q8N5V2 Number Species: 10
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q8N5V2 NP_001107562.1 710 82496 T504 Q Q M S G P K T S R T L R T K
Chimpanzee Pan troglodytes XP_516157 675 77421 T484 Q Q M S G P K T S R T L R T K
Rhesus Macaque Macaca mulatta XP_001114604 712 82436 T506 Q Q M S G P K T S R T L R T K
Dog Lupus familis XP_543291 742 85934 T536 Q Q M S G P K T S R T L R T K
Cat Felis silvestris
Mouse Mus musculus Q8CHT1 710 82180 T504 Q Q M S G P K T S R T L R T K
Rat Rattus norvegicus Q5BKC9 701 80989 T495 Q Q M S G P K T S R T L R T K
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001520926 611 70617 N424 E I Y L F L F N D L L V I C R
Chicken Gallus gallus NP_001010841 427 49944 I240 T K K L F R E I Y L F L F N D
Frog Xenopus laevis NP_001128552 856 97785 P660 M E L D M Q I P N S A G S K F
Zebra Danio Brachydanio rerio XP_697546 728 83194 S512 Q Q M S G P K S T R T M R S R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans NP_494706 1136 128641 S898 T Q L V E R R S S N I L Q S R
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 87.6 96.2 83.9 N.A. 86.1 86.1 N.A. 76 54.7 31.1 43.1 N.A. N.A. N.A. 25.9 N.A.
Protein Similarity: 100 89.5 97.6 88.8 N.A. 91.2 90.9 N.A. 81.5 57.7 48.9 62.5 N.A. N.A. N.A. 40.1 N.A.
P-Site Identity: 100 100 100 100 N.A. 100 100 N.A. 0 6.6 0 66.6 N.A. N.A. N.A. 20 N.A.
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. 20 20 20 100 N.A. N.A. N.A. 60 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 0 0 0 10 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 10 0 % C
% Asp: 0 0 0 10 0 0 0 0 10 0 0 0 0 0 10 % D
% Glu: 10 10 0 0 10 0 10 0 0 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 19 0 10 0 0 0 10 0 10 0 10 % F
% Gly: 0 0 0 0 64 0 0 0 0 0 0 10 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 10 0 0 0 0 10 10 0 0 10 0 10 0 0 % I
% Lys: 0 10 10 0 0 0 64 0 0 0 0 0 0 10 55 % K
% Leu: 0 0 19 19 0 10 0 0 0 19 10 73 0 0 0 % L
% Met: 10 0 64 0 10 0 0 0 0 0 0 10 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 10 10 10 0 0 0 10 0 % N
% Pro: 0 0 0 0 0 64 0 10 0 0 0 0 0 0 0 % P
% Gln: 64 73 0 0 0 10 0 0 0 0 0 0 10 0 0 % Q
% Arg: 0 0 0 0 0 19 10 0 0 64 0 0 64 0 28 % R
% Ser: 0 0 0 64 0 0 0 19 64 10 0 0 10 19 0 % S
% Thr: 19 0 0 0 0 0 0 55 10 0 64 0 0 55 0 % T
% Val: 0 0 0 10 0 0 0 0 0 0 0 10 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 10 0 0 0 0 0 10 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _