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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NGDN All Species: 34.85
Human Site: S250 Identified Species: 69.7
UniProt: Q8NEJ9 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q8NEJ9 NP_001036100.1 315 35894 S250 E S M M V R L S V S K R E K G
Chimpanzee Pan troglodytes XP_001163084 315 35789 S250 E S M M V R L S V S K R E K G
Rhesus Macaque Macaca mulatta XP_001108182 311 35188 S250 E S M M V R L S I S K R E K G
Dog Lupus familis XP_547734 318 36281 S253 E S M M V R L S V S K R E K G
Cat Felis silvestris
Mouse Mus musculus Q9DB96 315 35640 S250 E S M M V R L S V S K R E K G
Rat Rattus norvegicus NP_001100733 315 35795 S250 E S M M V R L S V S K R E K G
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001505953 316 35836 S251 E S M M V R L S V S K R E R G
Chicken Gallus gallus
Frog Xenopus laevis Q4KLC4 317 36090 N251 E S M M V R L N M T R K E K A
Zebra Danio Brachydanio rerio Q6PFJ1 315 36183 S253 E S M M V R L S T T R D Q K L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_608956 332 37306 V274 T Y L M R L P V T K A E K H R
Honey Bee Apis mellifera XP_625107 303 34687 T250 Y M T R L P V T K Q E K H R R
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001190632 421 47439 T352 N F L R L P V T K K D K Q R E
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99 94.9 91.8 N.A. 88.5 89.8 N.A. 81.6 N.A. 62.1 56.8 N.A. 41.5 41.9 N.A. 37
Protein Similarity: 100 99.3 97.1 94.9 N.A. 94.9 95.5 N.A. 91.1 N.A. 78.8 76.1 N.A. 62.3 62.8 N.A. 50.5
P-Site Identity: 100 100 93.3 100 N.A. 100 100 N.A. 93.3 N.A. 60 60 N.A. 6.6 0 N.A. 0
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. 100 N.A. 93.3 80 N.A. 20 40 N.A. 46.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 0 0 0 9 0 0 0 9 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 0 9 9 0 0 0 % D
% Glu: 75 0 0 0 0 0 0 0 0 0 9 9 67 0 9 % E
% Phe: 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 59 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 9 9 0 % H
% Ile: 0 0 0 0 0 0 0 0 9 0 0 0 0 0 0 % I
% Lys: 0 0 0 0 0 0 0 0 17 17 59 25 9 67 0 % K
% Leu: 0 0 17 0 17 9 75 0 0 0 0 0 0 0 9 % L
% Met: 0 9 75 84 0 0 0 0 9 0 0 0 0 0 0 % M
% Asn: 9 0 0 0 0 0 0 9 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 17 9 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 9 0 0 17 0 0 % Q
% Arg: 0 0 0 17 9 75 0 0 0 0 17 59 0 25 17 % R
% Ser: 0 75 0 0 0 0 0 67 0 59 0 0 0 0 0 % S
% Thr: 9 0 9 0 0 0 0 17 17 17 0 0 0 0 0 % T
% Val: 0 0 0 0 75 0 17 9 50 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 9 9 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _