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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ENGASE All Species: 7.88
Human Site: T224 Identified Species: 14.44
UniProt: Q8NFI3 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q8NFI3 NP_001036038.1 743 83987 T224 A D R L V Q I T Q F F R F D G
Chimpanzee Pan troglodytes XP_001159278 743 84023 T224 A D R L V Q I T Q F F R F D G
Rhesus Macaque Macaca mulatta XP_001107425 771 86711 A252 A D R L V Q I A Q F F R F D G
Dog Lupus familis XP_540466 711 79811 I200 F D G W L I N I E N S L S L A
Cat Felis silvestris
Mouse Mus musculus Q8BX80 734 82927 A216 A D R L V Q I A Q F F R F D G
Rat Rattus norvegicus
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001518787 252 28417
Chicken Gallus gallus P0C7A1 728 80871 A222 S H Q L A R I A Q H Y R F D G
Frog Xenopus laevis
Zebra Danio Brachydanio rerio A1L251 713 80236 S201 A D K L V Q I S H C N G F D G
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_573215 655 75729 V203 N A A H R H G V P V V G T F I
Honey Bee Apis mellifera XP_001121069 578 67421 G128 D H G V K I L G T V I T E K E
Nematode Worm Caenorhab. elegans NP_001021238 433 50225
Sea Urchin Strong. purpuratus XP_794341 764 85326 V219 K H D V Y V G V D I F G R N C
Poplar Tree Populus trichocarpa XP_002315137 698 78027 G209 T M H S S L P G S L V I W Y D
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.5 89.8 77.3 N.A. 78.7 N.A. N.A. 22 53.2 N.A. 47.6 N.A. 28.5 28.3 25.4 25.5
Protein Similarity: 100 99.7 92.6 82.9 N.A. 85.3 N.A. N.A. 26.1 65.8 N.A. 63.5 N.A. 43.6 43.2 37 42.1
P-Site Identity: 100 100 93.3 6.6 N.A. 93.3 N.A. N.A. 0 46.6 N.A. 60 N.A. 0 0 0 6.6
P-Site Similarity: 100 100 93.3 20 N.A. 93.3 N.A. N.A. 0 73.3 N.A. 73.3 N.A. 0 13.3 0 20
Percent
Protein Identity: 27.9 N.A. N.A. N.A. N.A. N.A.
Protein Similarity: 46.7 N.A. N.A. N.A. N.A. N.A.
P-Site Identity: 0 N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: 6.6 N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 39 8 8 0 8 0 0 24 0 0 0 0 0 0 8 % A
% Cys: 0 0 0 0 0 0 0 0 0 8 0 0 0 0 8 % C
% Asp: 8 47 8 0 0 0 0 0 8 0 0 0 0 47 8 % D
% Glu: 0 0 0 0 0 0 0 0 8 0 0 0 8 0 8 % E
% Phe: 8 0 0 0 0 0 0 0 0 31 39 0 47 8 0 % F
% Gly: 0 0 16 0 0 0 16 16 0 0 0 24 0 0 47 % G
% His: 0 24 8 8 0 8 0 0 8 8 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 16 47 8 0 8 8 8 0 0 8 % I
% Lys: 8 0 8 0 8 0 0 0 0 0 0 0 0 8 0 % K
% Leu: 0 0 0 47 8 8 8 0 0 8 0 8 0 8 0 % L
% Met: 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 8 0 0 0 0 0 8 0 0 8 8 0 0 8 0 % N
% Pro: 0 0 0 0 0 0 8 0 8 0 0 0 0 0 0 % P
% Gln: 0 0 8 0 0 39 0 0 39 0 0 0 0 0 0 % Q
% Arg: 0 0 31 0 8 8 0 0 0 0 0 39 8 0 0 % R
% Ser: 8 0 0 8 8 0 0 8 8 0 8 0 8 0 0 % S
% Thr: 8 0 0 0 0 0 0 16 8 0 0 8 8 0 0 % T
% Val: 0 0 0 16 39 8 0 16 0 16 16 0 0 0 0 % V
% Trp: 0 0 0 8 0 0 0 0 0 0 0 0 8 0 0 % W
% Tyr: 0 0 0 0 8 0 0 0 0 0 8 0 0 8 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _