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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ENGASE All Species: 18.79
Human Site: Y254 Identified Species: 34.44
UniProt: Q8NFI3 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q8NFI3 NP_001036038.1 743 83987 Y254 N M P P F L R Y L T T Q L H R
Chimpanzee Pan troglodytes XP_001159278 743 84023 Y254 N M P P F L R Y L T T Q L H R
Rhesus Macaque Macaca mulatta XP_001107425 771 86711 Y282 N T P P F L W Y L T T Q L H R
Dog Lupus familis XP_540466 711 79811 G230 L H E Q V P R G L V L W Y D S
Cat Felis silvestris
Mouse Mus musculus Q8BX80 734 82927 Y246 N T P L F L Q Y L T A Q L H Q
Rat Rattus norvegicus
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001518787 252 28417
Chicken Gallus gallus P0C7A1 728 80871 H252 N L S P F L R H L T A E V H G
Frog Xenopus laevis
Zebra Danio Brachydanio rerio A1L251 713 80236 Y231 N T G P F L R Y L T D Q M H E
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_573215 655 75729 A233 S V E S T V E A L T R L C E H
Honey Bee Apis mellifera XP_001121069 578 67421 V158 F A D A L I V V A K F Y K F D
Nematode Worm Caenorhab. elegans NP_001021238 433 50225 W13 I D T L E E L W S W K S R E D
Sea Urchin Strong. purpuratus XP_794341 764 85326 Y144 Q L L F F V Q Y L T K Q M H Q
Poplar Tree Populus trichocarpa XP_002315137 698 78027 D239 D K N K P F F D I C D G I F V
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.5 89.8 77.3 N.A. 78.7 N.A. N.A. 22 53.2 N.A. 47.6 N.A. 28.5 28.3 25.4 25.5
Protein Similarity: 100 99.7 92.6 82.9 N.A. 85.3 N.A. N.A. 26.1 65.8 N.A. 63.5 N.A. 43.6 43.2 37 42.1
P-Site Identity: 100 100 86.6 13.3 N.A. 66.6 N.A. N.A. 0 53.3 N.A. 66.6 N.A. 13.3 0 0 40
P-Site Similarity: 100 100 86.6 13.3 N.A. 80 N.A. N.A. 0 80 N.A. 73.3 N.A. 33.3 6.6 6.6 73.3
Percent
Protein Identity: 27.9 N.A. N.A. N.A. N.A. N.A.
Protein Similarity: 46.7 N.A. N.A. N.A. N.A. N.A.
P-Site Identity: 0 N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: 20 N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 8 0 8 0 0 0 8 8 0 16 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 8 0 0 8 0 0 % C
% Asp: 8 8 8 0 0 0 0 8 0 0 16 0 0 8 16 % D
% Glu: 0 0 16 0 8 8 8 0 0 0 0 8 0 16 8 % E
% Phe: 8 0 0 8 54 8 8 0 0 0 8 0 0 16 0 % F
% Gly: 0 0 8 0 0 0 0 8 0 0 0 8 0 0 8 % G
% His: 0 8 0 0 0 0 0 8 0 0 0 0 0 54 8 % H
% Ile: 8 0 0 0 0 8 0 0 8 0 0 0 8 0 0 % I
% Lys: 0 8 0 8 0 0 0 0 0 8 16 0 8 0 0 % K
% Leu: 8 16 8 16 8 47 8 0 70 0 8 8 31 0 0 % L
% Met: 0 16 0 0 0 0 0 0 0 0 0 0 16 0 0 % M
% Asn: 47 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 31 39 8 8 0 0 0 0 0 0 0 0 0 % P
% Gln: 8 0 0 8 0 0 16 0 0 0 0 47 0 0 16 % Q
% Arg: 0 0 0 0 0 0 39 0 0 0 8 0 8 0 24 % R
% Ser: 8 0 8 8 0 0 0 0 8 0 0 8 0 0 8 % S
% Thr: 0 24 8 0 8 0 0 0 0 62 24 0 0 0 0 % T
% Val: 0 8 0 0 8 16 8 8 0 8 0 0 8 0 8 % V
% Trp: 0 0 0 0 0 0 8 8 0 8 0 8 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 47 0 0 0 8 8 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _