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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ENGASE All Species: 20.91
Human Site: Y425 Identified Species: 38.33
UniProt: Q8NFI3 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q8NFI3 NP_001036038.1 743 83987 Y425 E E A V G P W Y H L S A Q E I
Chimpanzee Pan troglodytes XP_001159278 743 84023 Y425 E E A V G P W Y H L S A Q E I
Rhesus Macaque Macaca mulatta XP_001107425 771 86711 Y453 E E A V G P W Y H L S A Q E I
Dog Lupus familis XP_540466 711 79811 Y389 E E A E G P W Y H P S A Q E I
Cat Felis silvestris
Mouse Mus musculus Q8BX80 734 82927 Y417 E Q A V G P W Y H P S A Q E T
Rat Rattus norvegicus
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001518787 252 28417
Chicken Gallus gallus P0C7A1 728 80871 Y423 E E E A G P W Y N L S A Q E I
Frog Xenopus laevis
Zebra Danio Brachydanio rerio A1L251 713 80236 F405 V E T E R S W F N L H A Q E I
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_573215 655 75729 L385 P G W T F E T L N R F G Y N I
Honey Bee Apis mellifera XP_001121069 578 67421 F308 P G W T H E F F G S K T F Q E
Nematode Worm Caenorhab. elegans NP_001021238 433 50225 D163 K I D A G K I D L L I Q F C S
Sea Urchin Strong. purpuratus XP_794341 764 85326 C420 E V M K K A W C N L S S Q Q I
Poplar Tree Populus trichocarpa XP_002315137 698 78027 N399 T I E V F V E N K C N I G I G
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.5 89.8 77.3 N.A. 78.7 N.A. N.A. 22 53.2 N.A. 47.6 N.A. 28.5 28.3 25.4 25.5
Protein Similarity: 100 99.7 92.6 82.9 N.A. 85.3 N.A. N.A. 26.1 65.8 N.A. 63.5 N.A. 43.6 43.2 37 42.1
P-Site Identity: 100 100 100 86.6 N.A. 80 N.A. N.A. 0 80 N.A. 46.6 N.A. 6.6 0 13.3 40
P-Site Similarity: 100 100 100 86.6 N.A. 86.6 N.A. N.A. 0 86.6 N.A. 60 N.A. 13.3 20 20 60
Percent
Protein Identity: 27.9 N.A. N.A. N.A. N.A. N.A.
Protein Similarity: 46.7 N.A. N.A. N.A. N.A. N.A.
P-Site Identity: 6.6 N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: 13.3 N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 39 16 0 8 0 0 0 0 0 54 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 8 0 8 0 0 0 8 0 % C
% Asp: 0 0 8 0 0 0 0 8 0 0 0 0 0 0 0 % D
% Glu: 54 47 16 16 0 16 8 0 0 0 0 0 0 54 8 % E
% Phe: 0 0 0 0 16 0 8 16 0 0 8 0 16 0 0 % F
% Gly: 0 16 0 0 54 0 0 0 8 0 0 8 8 0 8 % G
% His: 0 0 0 0 8 0 0 0 39 0 8 0 0 0 0 % H
% Ile: 0 16 0 0 0 0 8 0 0 0 8 8 0 8 62 % I
% Lys: 8 0 0 8 8 8 0 0 8 0 8 0 0 0 0 % K
% Leu: 0 0 0 0 0 0 0 8 8 54 0 0 0 0 0 % L
% Met: 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 8 31 0 8 0 0 8 0 % N
% Pro: 16 0 0 0 0 47 0 0 0 16 0 0 0 0 0 % P
% Gln: 0 8 0 0 0 0 0 0 0 0 0 8 62 16 0 % Q
% Arg: 0 0 0 0 8 0 0 0 0 8 0 0 0 0 0 % R
% Ser: 0 0 0 0 0 8 0 0 0 8 54 8 0 0 8 % S
% Thr: 8 0 8 16 0 0 8 0 0 0 0 8 0 0 8 % T
% Val: 8 8 0 39 0 8 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 16 0 0 0 62 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 47 0 0 0 0 8 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _