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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: HMGB4 All Species: 9.09
Human Site: S99 Identified Species: 16.67
UniProt: Q8WW32 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.25
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q8WW32 NP_660206 186 22405 S99 A P R R P P S S F L L F C Q D
Chimpanzee Pan troglodytes Q9N1Q7 215 24747 R126 K D P N A P K R P P L A F F L
Rhesus Macaque Macaca mulatta XP_001107984 191 23044 S101 A P R R P P S S F L L F C Q D
Dog Lupus familis XP_851529 191 22584 S99 A P R R P P S S F L I F C Q D
Cat Felis silvestris
Mouse Mus musculus Q6P8W9 181 21585 K95 R D P K A P R K P P S S F L L
Rat Rattus norvegicus P52925 210 24140 A101 A P K R P P S A F F L F C S E
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus P26584 207 23809 A101 A P K R P P S A F F L F C S E
Frog Xenopus laevis NP_001079576 212 24227 A102 A P K R P P S A F F I F C S E
Zebra Danio Brachydanio rerio NP_001017769 198 22534 G100 A P R R P P S G F F L F C A E
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q09390 235 27301 A141 A P K R A L S A F F F Y S Q D
Sea Urchin Strong. purpuratus P40644 200 22815 A106 A P K R N L S A F F I F S G E
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P11633 99 11557 P13 E A K Q P K E P K K R T T R R
Red Bread Mold Neurospora crassa Q7S045 103 11590 R17 T T G K V E K R R A K K D P N
Conservation
Percent
Protein Identity: 100 29.7 93.7 79 N.A. 71.5 38.5 N.A. N.A. 39.1 37.2 37.3 N.A. N.A. N.A. 28.5 31
Protein Similarity: 100 48.3 95.2 86.9 N.A. 85.4 56.6 N.A. N.A. 57.9 55.1 55.5 N.A. N.A. N.A. 44.2 52
P-Site Identity: 100 13.3 100 93.3 N.A. 6.6 66.6 N.A. N.A. 66.6 60 73.3 N.A. N.A. N.A. 46.6 40
P-Site Similarity: 100 13.3 100 100 N.A. 13.3 86.6 N.A. N.A. 86.6 86.6 80 N.A. N.A. N.A. 66.6 66.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. 21.5 23.6
Protein Similarity: N.A. N.A. N.A. N.A. 32.2 36
P-Site Identity: N.A. N.A. N.A. N.A. 6.6 0
P-Site Similarity: N.A. N.A. N.A. N.A. 26.6 13.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 70 8 0 0 24 0 0 39 0 8 0 8 0 8 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 54 0 0 % C
% Asp: 0 16 0 0 0 0 0 0 0 0 0 0 8 0 31 % D
% Glu: 8 0 0 0 0 8 8 0 0 0 0 0 0 0 39 % E
% Phe: 0 0 0 0 0 0 0 0 70 47 8 62 16 8 0 % F
% Gly: 0 0 8 0 0 0 0 8 0 0 0 0 0 8 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 24 0 0 0 0 % I
% Lys: 8 0 47 16 0 8 16 8 8 8 8 8 0 0 0 % K
% Leu: 0 0 0 0 0 16 0 0 0 24 47 0 0 8 16 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 8 8 0 0 0 0 0 0 0 0 0 8 % N
% Pro: 0 70 16 0 62 70 0 8 16 16 0 0 0 8 0 % P
% Gln: 0 0 0 8 0 0 0 0 0 0 0 0 0 31 0 % Q
% Arg: 8 0 31 70 0 0 8 16 8 0 8 0 0 8 8 % R
% Ser: 0 0 0 0 0 0 70 24 0 0 8 8 16 24 0 % S
% Thr: 8 8 0 0 0 0 0 0 0 0 0 8 8 0 0 % T
% Val: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _