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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ROBO4 All Species: 10.3
Human Site: S533 Identified Species: 32.38
UniProt: Q8WZ75 Number Species: 7
    Phosphosite Substitution
    Charge Score: -0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q8WZ75 NP_061928.4 1007 107457 S533 A D T W R S T S G S R D L S S
Chimpanzee Pan troglodytes XP_508841 908 97520 L458 S E H G P W T L E Q L R A T L
Rhesus Macaque Macaca mulatta XP_001106913 1300 139057 W576 K N S I T L T W E P N P Q T G
Dog Lupus familis XP_546424 1050 111614 S582 A D T W R S T S G S R D L S S
Cat Felis silvestris
Mouse Mus musculus Q8C310 1012 108481 S534 A D T W R S T S G S R D L S S
Rat Rattus norvegicus Q80W87 961 102562 C499 L L L G I A V C I Y R R R K A
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001516844 829 87327 R380 R L A P Q L A R L S S P W P S
Chicken Gallus gallus
Frog Xenopus laevis NP_001164069 1396 153618 S931 R P G M L A T S A S N Y P W L
Zebra Danio Brachydanio rerio
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 87.9 23.7 80.6 N.A. 77.1 73.5 N.A. 42.2 N.A. 22 N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: 100 88.9 34.9 84.9 N.A. 84.4 79.4 N.A. 50.7 N.A. 36.9 N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: 100 6.6 6.6 100 N.A. 100 6.6 N.A. 13.3 N.A. 20 N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: 100 26.6 26.6 100 N.A. 100 20 N.A. 20 N.A. 26.6 N.A. N.A. N.A. N.A. N.A. N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 38 0 13 0 0 25 13 0 13 0 0 0 13 0 13 % A
% Cys: 0 0 0 0 0 0 0 13 0 0 0 0 0 0 0 % C
% Asp: 0 38 0 0 0 0 0 0 0 0 0 38 0 0 0 % D
% Glu: 0 13 0 0 0 0 0 0 25 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 13 25 0 0 0 0 38 0 0 0 0 0 13 % G
% His: 0 0 13 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 13 13 0 0 0 13 0 0 0 0 0 0 % I
% Lys: 13 0 0 0 0 0 0 0 0 0 0 0 0 13 0 % K
% Leu: 13 25 13 0 13 25 0 13 13 0 13 0 38 0 25 % L
% Met: 0 0 0 13 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 13 0 0 0 0 0 0 0 0 25 0 0 0 0 % N
% Pro: 0 13 0 13 13 0 0 0 0 13 0 25 13 13 0 % P
% Gln: 0 0 0 0 13 0 0 0 0 13 0 0 13 0 0 % Q
% Arg: 25 0 0 0 38 0 0 13 0 0 50 25 13 0 0 % R
% Ser: 13 0 13 0 0 38 0 50 0 63 13 0 0 38 50 % S
% Thr: 0 0 38 0 13 0 75 0 0 0 0 0 0 25 0 % T
% Val: 0 0 0 0 0 0 13 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 38 0 13 0 13 0 0 0 0 13 13 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 13 0 13 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _