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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP1MT All Species: 13.94
Human Site: S25 Identified Species: 21.9
UniProt: Q969P6 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q969P6 NP_443195.1 601 69872 S25 E V P R R P A S R G V P G S R
Chimpanzee Pan troglodytes A9Q1D5 601 69739 S25 E V P R R P A S R G V P G S R
Rhesus Macaque Macaca mulatta XP_001088021 767 90935 D87 K D K H K D R D K E K R K E E
Dog Lupus familis XP_534420 1004 115318 R280 K K D K E K D R E K S K H S N
Cat Felis silvestris
Mouse Mus musculus Q8R4U6 593 68985 S22 H V P C R V P S C Q V S R V A
Rat Rattus norvegicus Q6IM78 593 68993 S22 H V P R R V P S R Q V S R G S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519186 689 80888 S100 K L A S R K S S R K G K S K N
Chicken Gallus gallus NP_001001300 593 69066 F19 R G V P R P P F I P T A L R A
Frog Xenopus laevis P41512 829 98212 E229 K K R K Q E E E D I K P K K K
Zebra Danio Brachydanio rerio NP_001037789 758 89767 R63 D S S E K K H R D K D K E R Q
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P30189 972 111670 S79 S K E H K S S S R D K D R H K
Honey Bee Apis mellifera XP_396203 973 114099 S102 E K D K H D T S N S N K D K D
Nematode Worm Caenorhab. elegans O17966 806 94011 K100 D D Y K P E K K K S K K N N K
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30181 916 102780 S141 G N K G P T S S R Q V S S P Q
Baker's Yeast Sacchar. cerevisiae P04786 769 89977 D96 K I K K E D G D V K V K T T K
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.8 53.9 43.5 N.A. 73.5 75 N.A. 67.7 73.5 48.1 54.8 N.A. 38.5 40.7 45 N.A.
Protein Similarity: 100 99.5 67.2 52.3 N.A. 83.6 84.8 N.A. 78.5 86.3 60.3 67.1 N.A. 47.9 49.5 57.4 N.A.
P-Site Identity: 100 100 0 6.6 N.A. 33.3 46.6 N.A. 20 13.3 6.6 0 N.A. 13.3 13.3 0 N.A.
P-Site Similarity: 100 100 20 20 N.A. 33.3 46.6 N.A. 40 13.3 33.3 20 N.A. 33.3 20 33.3 N.A.
Percent
Protein Identity: N.A. N.A. N.A. 32.5 40.7 N.A.
Protein Similarity: N.A. N.A. N.A. 45.4 55.9 N.A.
P-Site Identity: N.A. N.A. N.A. 20 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 33.3 40 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 0 0 0 14 0 0 0 0 7 0 0 14 % A
% Cys: 0 0 0 7 0 0 0 0 7 0 0 0 0 0 0 % C
% Asp: 14 14 14 0 0 20 7 14 14 7 7 7 7 0 7 % D
% Glu: 20 0 7 7 14 14 7 7 7 7 0 0 7 7 7 % E
% Phe: 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 % F
% Gly: 7 7 0 7 0 0 7 0 0 14 7 0 14 7 0 % G
% His: 14 0 0 14 7 0 7 0 0 0 0 0 7 7 0 % H
% Ile: 0 7 0 0 0 0 0 0 7 7 0 0 0 0 0 % I
% Lys: 34 27 20 34 20 20 7 7 14 27 27 40 14 20 27 % K
% Leu: 0 7 0 0 0 0 0 0 0 0 0 0 7 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 7 0 0 0 0 0 0 7 0 7 0 7 7 14 % N
% Pro: 0 0 27 7 14 20 20 0 0 7 0 20 0 7 0 % P
% Gln: 0 0 0 0 7 0 0 0 0 20 0 0 0 0 14 % Q
% Arg: 7 0 7 20 40 0 7 14 40 0 0 7 20 14 14 % R
% Ser: 7 7 7 7 0 7 20 54 0 14 7 20 14 20 7 % S
% Thr: 0 0 0 0 0 7 7 0 0 0 7 0 7 7 0 % T
% Val: 0 27 7 0 0 14 0 0 7 0 40 0 0 7 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _