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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP1MT All Species: 24.85
Human Site: S516 Identified Species: 39.05
UniProt: Q969P6 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q969P6 NP_443195.1 601 69872 S516 K A Q G D G K S R S V L E K K
Chimpanzee Pan troglodytes A9Q1D5 601 69739 S516 K A Q G D G K S R S V L E K K
Rhesus Macaque Macaca mulatta XP_001088021 767 90935 T682 K V M K D A K T K K V V E S K
Dog Lupus familis XP_534420 1004 115318 T919 K V L K D A K T K K V V E S K
Cat Felis silvestris
Mouse Mus musculus Q8R4U6 593 68985 P509 R M R G D S K P K S F L Q K Q
Rat Rattus norvegicus Q6IM78 593 68993 S509 R M R G D S K S K S F L Q K Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519186 689 80888 S604 K A K K D I K S K S N L E K K
Chicken Gallus gallus NP_001001300 593 69066 A508 K D K K D A K A E A N V Q K K
Frog Xenopus laevis P41512 829 98212 T736 K V R R D E K T K K L V E S K
Zebra Danio Brachydanio rerio NP_001037789 758 89767 F673 K V R K D E K F K K A V E A K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P30189 972 111670 S889 K C E S E Y H S R D E K K G K
Honey Bee Apis mellifera XP_396203 973 114099 D889 A I N D A E L D S T Y E K K K
Nematode Worm Caenorhab. elegans O17966 806 94011 S716 E A E A A L K S A R G A E K E
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30181 916 102780 P833 K K I R S L E P N A W E K K I
Baker's Yeast Sacchar. cerevisiae P04786 769 89977 L674 K Q E F E K E L K T G E V E L
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.8 53.9 43.5 N.A. 73.5 75 N.A. 67.7 73.5 48.1 54.8 N.A. 38.5 40.7 45 N.A.
Protein Similarity: 100 99.5 67.2 52.3 N.A. 83.6 84.8 N.A. 78.5 86.3 60.3 67.1 N.A. 47.9 49.5 57.4 N.A.
P-Site Identity: 100 100 40 40 N.A. 40 46.6 N.A. 66.6 33.3 33.3 33.3 N.A. 26.6 13.3 33.3 N.A.
P-Site Similarity: 100 100 60 60 N.A. 73.3 80 N.A. 80 66.6 66.6 53.3 N.A. 46.6 26.6 53.3 N.A.
Percent
Protein Identity: N.A. N.A. N.A. 32.5 40.7 N.A.
Protein Similarity: N.A. N.A. N.A. 45.4 55.9 N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 33.3 46.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 27 0 7 14 20 0 7 7 14 7 7 0 7 0 % A
% Cys: 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 7 0 7 67 0 0 7 0 7 0 0 0 0 0 % D
% Glu: 7 0 20 0 14 20 14 0 7 0 7 20 54 7 7 % E
% Phe: 0 0 0 7 0 0 0 7 0 0 14 0 0 0 0 % F
% Gly: 0 0 0 27 0 14 0 0 0 0 14 0 0 7 0 % G
% His: 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 % H
% Ile: 0 7 7 0 0 7 0 0 0 0 0 0 0 0 7 % I
% Lys: 74 7 14 34 0 7 74 0 54 27 0 7 20 60 67 % K
% Leu: 0 0 7 0 0 14 7 7 0 0 7 34 0 0 7 % L
% Met: 0 14 7 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 7 0 0 0 0 0 7 0 14 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 14 0 0 0 0 0 0 0 % P
% Gln: 0 7 14 0 0 0 0 0 0 0 0 0 20 0 14 % Q
% Arg: 14 0 27 14 0 0 0 0 20 7 0 0 0 0 0 % R
% Ser: 0 0 0 7 7 14 0 40 7 34 0 0 0 20 0 % S
% Thr: 0 0 0 0 0 0 0 20 0 14 0 0 0 0 0 % T
% Val: 0 27 0 0 0 0 0 0 0 0 27 34 7 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 % W
% Tyr: 0 0 0 0 0 7 0 0 0 0 7 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _