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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
NSUN4
All Species:
35.76
Human Site:
S219
Identified Species:
60.51
UniProt:
Q96CB9
Number Species:
13
Phosphosite Substitution
Charge Score:
0.08
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
Q96CB9
NP_950245.2
384
43089
S219
R
L
Q
K
I
L
H
S
Y
V
P
E
E
I
R
Chimpanzee
Pan troglodytes
XP_513142
833
91470
S219
R
L
Q
K
I
L
H
S
Y
V
P
E
E
I
R
Rhesus Macaque
Macaca mulatta
XP_001108301
384
43099
S219
R
L
Q
N
I
L
H
S
Y
V
P
Q
E
I
R
Dog
Lupus familis
XP_853047
384
43059
S219
R
L
Q
R
V
L
R
S
Y
V
P
Q
D
I
R
Cat
Felis silvestris
Mouse
Mus musculus
Q9CZ57
381
42835
S216
R
L
Q
K
V
L
H
S
Y
V
P
Q
D
I
R
Rat
Rattus norvegicus
NP_001100148
381
42594
S216
R
L
Q
K
V
L
H
S
Y
V
P
Q
D
I
R
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
XP_001518738
248
26969
N96
A
D
R
P
G
L
P
N
L
L
C
Y
T
F
R
Chicken
Gallus gallus
XP_422449
404
44758
S242
R
L
Y
H
V
L
N
S
Y
I
P
K
E
V
R
Frog
Xenopus laevis
Q5M7E3
406
46042
S241
R
L
H
R
V
L
H
S
Y
V
P
R
D
Q
R
Zebra Danio
Brachydanio rerio
Q4KMK0
367
41244
S208
W
L
L
K
T
L
E
S
Y
V
P
P
S
L
R
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
NP_608573
503
57519
E336
K
L
R
K
V
M
Q
E
Y
L
F
D
Y
K
E
Honey Bee
Apis mellifera
XP_394395
493
56346
Q329
R
I
K
N
V
M
N
Q
F
V
S
N
I
C
E
Nematode Worm
Caenorhab. elegans
NP_490958
465
51501
T298
Q
L
K
R
A
L
M
T
Y
V
P
E
D
S
E
Sea Urchin
Strong. purpuratus
XP_792601
513
57508
L348
R
L
Q
E
V
L
G
L
C
L
P
R
S
I
R
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Baker's Yeast
Sacchar. cerevisiae
Red Bread Mold
Neurospora crassa
Conservation
Percent
Protein Identity:
100
44.7
96.6
84.9
N.A.
85.9
83.5
N.A.
38.5
61.1
59.3
35.1
N.A.
32.5
32.4
31.3
35.4
Protein Similarity:
100
45.9
98.4
91.4
N.A.
91.9
90.6
N.A.
47.9
74.5
72.6
54.1
N.A.
48.3
47.6
46.8
49.3
P-Site Identity:
100
100
86.6
66.6
N.A.
80
80
N.A.
13.3
53.3
60
53.3
N.A.
20
13.3
40
46.6
P-Site Similarity:
100
100
93.3
93.3
N.A.
100
100
N.A.
33.3
86.6
80
60
N.A.
60
53.3
73.3
66.6
Percent
Protein Identity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
Protein Similarity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
P-Site Identity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
P-Site Similarity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
8
0
0
0
8
0
0
0
0
0
0
0
0
0
0
% A
% Cys:
0
0
0
0
0
0
0
0
8
0
8
0
0
8
0
% C
% Asp:
0
8
0
0
0
0
0
0
0
0
0
8
36
0
0
% D
% Glu:
0
0
0
8
0
0
8
8
0
0
0
22
29
0
22
% E
% Phe:
0
0
0
0
0
0
0
0
8
0
8
0
0
8
0
% F
% Gly:
0
0
0
0
8
0
8
0
0
0
0
0
0
0
0
% G
% His:
0
0
8
8
0
0
43
0
0
0
0
0
0
0
0
% H
% Ile:
0
8
0
0
22
0
0
0
0
8
0
0
8
50
0
% I
% Lys:
8
0
15
43
0
0
0
0
0
0
0
8
0
8
0
% K
% Leu:
0
86
8
0
0
86
0
8
8
22
0
0
0
8
0
% L
% Met:
0
0
0
0
0
15
8
0
0
0
0
0
0
0
0
% M
% Asn:
0
0
0
15
0
0
15
8
0
0
0
8
0
0
0
% N
% Pro:
0
0
0
8
0
0
8
0
0
0
79
8
0
0
0
% P
% Gln:
8
0
50
0
0
0
8
8
0
0
0
29
0
8
0
% Q
% Arg:
72
0
15
22
0
0
8
0
0
0
0
15
0
0
79
% R
% Ser:
0
0
0
0
0
0
0
65
0
0
8
0
15
8
0
% S
% Thr:
0
0
0
0
8
0
0
8
0
0
0
0
8
0
0
% T
% Val:
0
0
0
0
58
0
0
0
0
72
0
0
0
8
0
% V
% Trp:
8
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% W
% Tyr:
0
0
8
0
0
0
0
0
79
0
0
8
8
0
0
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _