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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NSUN5 All Species: 19.7
Human Site: T325 Identified Species: 28.89
UniProt: Q96P11 Number Species: 15
    Phosphosite Substitution
    Charge Score: -0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q96P11 NP_060514.1 429 46692 T325 L E E P G A G T P S P V R L H
Chimpanzee Pan troglodytes XP_519138 437 47282 T325 L E E P G A G T P S P V R L H
Rhesus Macaque Macaca mulatta XP_001110692 429 46730 T325 L E E P G A G T P S P A R L H
Dog Lupus familis XP_536846 469 51185 T325 L E E P G T G T P S K A R L Q
Cat Felis silvestris
Mouse Mus musculus Q8K4F6 465 51011 T325 L E E H G E G T P S K E R L Q
Rat Rattus norvegicus XP_213749 451 49532 T323 L E E H G E G T P S K E R L Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514526 433 47383 G293 L E E P E A D G P S P E R L R
Chicken Gallus gallus XP_415710 465 50976 S325 P L D E A A P S A E R L Q A L
Frog Xenopus laevis
Zebra Danio Brachydanio rerio NP_998450 435 49024 E323 D E L P E T Q E D G R L Q A L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_393743 578 66348 G412 I V Y G K E K G D P Q R L K Q
Nematode Worm Caenorhab. elegans NP_497089 439 49820 A333 D E I T G G N A E K E R L E K
Sea Urchin Strong. purpuratus XP_787860 508 56859 M348 D Y L V D D D M Q E D R A R L
Poplar Tree Populus trichocarpa XP_002309481 433 47775 R330 L D H L L P S R T T D V V D T
Maize Zea mays NP_001151492 498 55261 H348 L D Y L L P S H S R D S Q D D
Rice Oryza sativa
Thale Cress Arabidopsis thaliana NP_851079 567 63156 H403 L D H L L P S H S E D N N M N
Baker's Yeast Sacchar. cerevisiae P53972 490 56159 R347 F F D S F N R R K I D D K D D
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 89.4 96.9 81.2 N.A. 81.7 83.8 N.A. 73.4 61 N.A. 57.9 N.A. N.A. 29.2 35.7 38.9
Protein Similarity: 100 90.6 98.5 85.7 N.A. 86.2 88.6 N.A. 80.1 72.9 N.A. 71 N.A. N.A. 43.5 55.1 55.3
P-Site Identity: 100 100 93.3 73.3 N.A. 66.6 66.6 N.A. 66.6 6.6 N.A. 13.3 N.A. N.A. 0 13.3 0
P-Site Similarity: 100 100 93.3 73.3 N.A. 66.6 66.6 N.A. 66.6 33.3 N.A. 26.6 N.A. N.A. 6.6 13.3 0
Percent
Protein Identity: 33.2 30.7 N.A. 28.9 28.5 N.A.
Protein Similarity: 50.3 44.3 N.A. 42.1 48.3 N.A.
P-Site Identity: 13.3 6.6 N.A. 6.6 0 N.A.
P-Site Similarity: 26.6 20 N.A. 26.6 13.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 7 32 0 7 7 0 0 13 7 13 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 19 19 13 0 7 7 13 0 13 0 32 7 0 19 13 % D
% Glu: 0 57 44 7 13 19 0 7 7 19 7 19 0 7 0 % E
% Phe: 7 7 0 0 7 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 7 44 7 38 13 0 7 0 0 0 0 0 % G
% His: 0 0 13 13 0 0 0 13 0 0 0 0 0 0 19 % H
% Ile: 7 0 7 0 0 0 0 0 0 7 0 0 0 0 0 % I
% Lys: 0 0 0 0 7 0 7 0 7 7 19 0 7 7 7 % K
% Leu: 63 7 13 19 19 0 0 0 0 0 0 13 13 44 19 % L
% Met: 0 0 0 0 0 0 0 7 0 0 0 0 0 7 0 % M
% Asn: 0 0 0 0 0 7 7 0 0 0 0 7 7 0 7 % N
% Pro: 7 0 0 38 0 19 7 0 44 7 25 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 7 0 7 0 7 0 19 0 25 % Q
% Arg: 0 0 0 0 0 0 7 13 0 7 13 19 44 7 7 % R
% Ser: 0 0 0 7 0 0 19 7 13 44 0 7 0 0 0 % S
% Thr: 0 0 0 7 0 13 0 38 7 7 0 0 0 0 7 % T
% Val: 0 7 0 7 0 0 0 0 0 0 0 19 7 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 7 13 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _