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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: GAD1 All Species: 32.73
Human Site: S249 Identified Species: 55.38
UniProt: Q99259 Number Species: 13
    Phosphosite Substitution
    Charge Score: -0.15
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q99259 NP_000808.2 594 66897 S249 K D G D G I F S P G G A I S N
Chimpanzee Pan troglodytes Q5IS68 594 66879 S249 K D G D G I F S P G G A I S N
Rhesus Macaque Macaca mulatta XP_001082995 594 66721 S249 K D G D G I F S P G G A I S N
Dog Lupus familis XP_541080 543 61384 S242 V S E Q G H Y S V K K A A A I
Cat Felis silvestris
Mouse Mus musculus P48318 593 66630 S248 K D G D G I F S P G G A I S N
Rat Rattus norvegicus P18088 593 66622 S248 K D G D G I F S P G G A I S N
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514987 594 67148 S249 K D G D G I F S P G G A I S N
Chicken Gallus gallus NP_990244 590 66692 S245 K D G D G I F S P G G A I S N
Frog Xenopus laevis NP_001079270 563 64077 K241 Y K Y F P E V K T K G M A A V
Zebra Danio Brachydanio rerio NP_919400 587 66137 S242 G D G D A L F S P G G A I S N
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P20228 510 57801 G220 C A A V C G L G T D H C I V V
Honey Bee Apis mellifera XP_391979 509 57849 G219 C A A V C G L G T D N C V M V
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_784856 614 68930 G268 G D G I F A P G G A V C N L Y
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9M0G4 545 60786 R249 D Q T H S S F R K A C L I G G
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.4 97.9 59.7 N.A. 97.1 97.1 N.A. 92.4 92.4 69.1 83.1 N.A. 50.6 49.8 N.A. 55
Protein Similarity: 100 100 98.4 75.5 N.A. 98.8 98.8 N.A. 96.1 96.4 77.9 91.4 N.A. 64.8 66.8 N.A. 71.3
P-Site Identity: 100 100 100 20 N.A. 100 100 N.A. 100 100 6.6 80 N.A. 6.6 0 N.A. 13.3
P-Site Similarity: 100 100 100 33.3 N.A. 100 100 N.A. 100 100 13.3 86.6 N.A. 6.6 6.6 N.A. 13.3
Percent
Protein Identity: N.A. N.A. N.A. 22.7 N.A. N.A.
Protein Similarity: N.A. N.A. N.A. 38.7 N.A. N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. 13.3 N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 15 15 0 8 8 0 0 0 15 0 65 15 15 0 % A
% Cys: 15 0 0 0 15 0 0 0 0 0 8 22 0 0 0 % C
% Asp: 8 65 0 58 0 0 0 0 0 15 0 0 0 0 0 % D
% Glu: 0 0 8 0 0 8 0 0 0 0 0 0 0 0 0 % E
% Phe: 0 0 0 8 8 0 65 0 0 0 0 0 0 0 0 % F
% Gly: 15 0 65 0 58 15 0 22 8 58 65 0 0 8 8 % G
% His: 0 0 0 8 0 8 0 0 0 0 8 0 0 0 0 % H
% Ile: 0 0 0 8 0 50 0 0 0 0 0 0 72 0 8 % I
% Lys: 50 8 0 0 0 0 0 8 8 15 8 0 0 0 0 % K
% Leu: 0 0 0 0 0 8 15 0 0 0 0 8 0 8 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 8 0 8 0 % M
% Asn: 0 0 0 0 0 0 0 0 0 0 8 0 8 0 58 % N
% Pro: 0 0 0 0 8 0 8 0 58 0 0 0 0 0 0 % P
% Gln: 0 8 0 8 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 0 0 0 0 0 0 8 0 0 0 0 0 0 0 % R
% Ser: 0 8 0 0 8 8 0 65 0 0 0 0 0 58 0 % S
% Thr: 0 0 8 0 0 0 0 0 22 0 0 0 0 0 0 % T
% Val: 8 0 0 15 0 0 8 0 8 0 8 0 8 8 22 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 8 0 8 0 0 0 8 0 0 0 0 0 0 0 8 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _