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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NOTCH4 All Species: 7.88
Human Site: S1849 Identified Species: 14.44
UniProt: Q99466 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.17
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q99466 NP_004548.3 2003 209622 S1849 R A R T V S V S V P P H G G G
Chimpanzee Pan troglodytes XP_518380 2003 209553 S1844 L A R T V S V S V P P H G G G
Rhesus Macaque Macaca mulatta XP_001114248 2471 265649 S2166 T Y V S D T T S S P M I T S P
Dog Lupus familis XP_538847 1778 185807 E1643 T A A R R L L E A G A N P N Q
Cat Felis silvestris
Mouse Mus musculus P31695 1964 206674 L1811 Q R S H W D L L T L L E G A G
Rat Rattus norvegicus Q07008 2531 270981 G2233 G M P D T H L G I S H L N V A
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001512292 2467 261963 S2143 K G Q L S E S S V T L S P V D
Chicken Gallus gallus XP_415420 2590 279561 N2270 Q S P S M P L N H L P G M P D
Frog Xenopus laevis P21783 2524 275106 T2120 Y N L V K S P T L H N G P L G
Zebra Danio Brachydanio rerio P46530 2437 262289 N2249 S H L P V A N N V G G A Q A G
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P07207 2703 288835 A2237 T S A A S K K A A N L N G L N
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P14585 1429 157097 D1294 H N I V D I F D R C R P E R E
Sea Urchin Strong. purpuratus P10079 1064 112055 I929 C Q C R L G Y I G V N C E E V
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99 33.6 78.1 N.A. 80.1 34 N.A. 33.4 34.4 33.4 35.9 N.A. 30.2 N.A. 22.9 23.4
Protein Similarity: 100 99.3 46.5 81.7 N.A. 85.7 45.9 N.A. 45.4 45.8 46 47.7 N.A. 40.6 N.A. 36 32
P-Site Identity: 100 93.3 13.3 6.6 N.A. 13.3 0 N.A. 13.3 6.6 13.3 20 N.A. 6.6 N.A. 0 0
P-Site Similarity: 100 93.3 26.6 20 N.A. 26.6 13.3 N.A. 26.6 46.6 26.6 33.3 N.A. 26.6 N.A. 0 6.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 24 16 8 0 8 0 8 16 0 8 8 0 16 8 % A
% Cys: 8 0 8 0 0 0 0 0 0 8 0 8 0 0 0 % C
% Asp: 0 0 0 8 16 8 0 8 0 0 0 0 0 0 16 % D
% Glu: 0 0 0 0 0 8 0 8 0 0 0 8 16 8 8 % E
% Phe: 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 % F
% Gly: 8 8 0 0 0 8 0 8 8 16 8 16 31 16 39 % G
% His: 8 8 0 8 0 8 0 0 8 8 8 16 0 0 0 % H
% Ile: 0 0 8 0 0 8 0 8 8 0 0 8 0 0 0 % I
% Lys: 8 0 0 0 8 8 8 0 0 0 0 0 0 0 0 % K
% Leu: 8 0 16 8 8 8 31 8 8 16 24 8 0 16 0 % L
% Met: 0 8 0 0 8 0 0 0 0 0 8 0 8 0 0 % M
% Asn: 0 16 0 0 0 0 8 16 0 8 16 16 8 8 8 % N
% Pro: 0 0 16 8 0 8 8 0 0 24 24 8 24 8 8 % P
% Gln: 16 8 8 0 0 0 0 0 0 0 0 0 8 0 8 % Q
% Arg: 8 8 16 16 8 0 0 0 8 0 8 0 0 8 0 % R
% Ser: 8 16 8 16 16 24 8 31 8 8 0 8 0 8 0 % S
% Thr: 24 0 0 16 8 8 8 8 8 8 0 0 8 0 0 % T
% Val: 0 0 8 16 24 0 16 0 31 8 0 0 0 16 8 % V
% Trp: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 8 8 0 0 0 0 8 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _