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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NOTCH4 All Species: 7.88
Human Site: T1817 Identified Species: 14.44
UniProt: Q99466 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q99466 NP_004548.3 2003 209622 T1817 R N H W D L L T L L E G A G P
Chimpanzee Pan troglodytes XP_518380 2003 209553 T1812 R N H W D L L T L L E G A G P
Rhesus Macaque Macaca mulatta XP_001114248 2471 265649 S2134 K G S R R K K S L S E K V Q L
Dog Lupus familis XP_538847 1778 185807 G1611 W E P L L G G G A C P Q A H T
Cat Felis silvestris
Mouse Mus musculus P31695 1964 206674 Q1779 E G A V E V A Q L L L E L G A
Rat Rattus norvegicus Q07008 2531 270981 S2201 E S P H G Y L S D V A S P P L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001512292 2467 261963 S2111 S A K G A V P S S L P N L A R
Chicken Gallus gallus XP_415420 2590 279561 V2238 N S S V L S P V D S L E S P H
Frog Xenopus laevis P21783 2524 275106 D2088 Y A N R D I T D H M D R L P R
Zebra Danio Brachydanio rerio P46530 2437 262289 Q2217 P I S L N Q L Q G L A D S H M
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P07207 2703 288835 K2205 Q K A A K K A K L I E G S P D
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P14585 1429 157097 Q1262 E V V M Y L I Q Q G A S V E A
Sea Urchin Strong. purpuratus P10079 1064 112055 I897 Y A G Q N C E I D I N E C A S
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99 33.6 78.1 N.A. 80.1 34 N.A. 33.4 34.4 33.4 35.9 N.A. 30.2 N.A. 22.9 23.4
Protein Similarity: 100 99.3 46.5 81.7 N.A. 85.7 45.9 N.A. 45.4 45.8 46 47.7 N.A. 40.6 N.A. 36 32
P-Site Identity: 100 100 13.3 6.6 N.A. 20 6.6 N.A. 6.6 0 6.6 13.3 N.A. 20 N.A. 6.6 0
P-Site Similarity: 100 100 26.6 6.6 N.A. 33.3 26.6 N.A. 20 13.3 33.3 26.6 N.A. 40 N.A. 13.3 13.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 24 16 8 8 0 16 0 8 0 24 0 24 16 16 % A
% Cys: 0 0 0 0 0 8 0 0 0 8 0 0 8 0 0 % C
% Asp: 0 0 0 0 24 0 0 8 24 0 8 8 0 0 8 % D
% Glu: 24 8 0 0 8 0 8 0 0 0 31 24 0 8 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 16 8 8 8 8 8 8 8 8 0 24 0 24 0 % G
% His: 0 0 16 8 0 0 0 0 8 0 0 0 0 16 8 % H
% Ile: 0 8 0 0 0 8 8 8 0 16 0 0 0 0 0 % I
% Lys: 8 8 8 0 8 16 8 8 0 0 0 8 0 0 0 % K
% Leu: 0 0 0 16 16 24 31 0 39 39 16 0 24 0 16 % L
% Met: 0 0 0 8 0 0 0 0 0 8 0 0 0 0 8 % M
% Asn: 8 16 8 0 16 0 0 0 0 0 8 8 0 0 0 % N
% Pro: 8 0 16 0 0 0 16 0 0 0 16 0 8 31 16 % P
% Gln: 8 0 0 8 0 8 0 24 8 0 0 8 0 8 0 % Q
% Arg: 16 0 0 16 8 0 0 0 0 0 0 8 0 0 16 % R
% Ser: 8 16 24 0 0 8 0 24 8 16 0 16 24 0 8 % S
% Thr: 0 0 0 0 0 0 8 16 0 0 0 0 0 0 8 % T
% Val: 0 8 8 16 0 16 0 8 0 8 0 0 16 0 0 % V
% Trp: 8 0 0 16 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 16 0 0 0 8 8 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _