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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG5 All Species: 16.97
Human Site: S225 Identified Species: 26.67
UniProt: Q9H1Y0 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9H1Y0 NP_004840.1 275 32447 S225 L L K E V C P S A I D P E D G
Chimpanzee Pan troglodytes XP_001144721 237 28161 K197 D P E D G E K K N Q V M I H G
Rhesus Macaque Macaca mulatta XP_001087825 249 29319 P205 F I Q K L F R P V A A D G Q L
Dog Lupus familis XP_854294 275 32353 S225 L L K E V C P S A I A P E D G
Cat Felis silvestris
Mouse Mus musculus Q99J83 275 32384 S225 L L R E V C P S A V A P E D G
Rat Rattus norvegicus Q3MQ06 275 32380 S225 L L R E V C P S A V A P E D G
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001512232 230 26918 Y190 R Y I P F R I Y Q P L S A A T
Chicken Gallus gallus NP_001006409 275 32460 S225 L L K D V C P S A I T P E D G
Frog Xenopus laevis NP_001085331 306 35845 A256 L I R E I C P A A V P T E D E
Zebra Danio Brachydanio rerio NP_991181 275 32097 A227 L L K E L F P A A I E D E P K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q9W3R7 269 31450 P226 L M A E L S T P V R R A V G C
Honey Bee Apis mellifera XP_623456 265 31393 V223 L R H L L N E V F P D N E N I
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001204016 250 29681 K206 I F A D P D T K D Y K V L I H
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9FFI2 337 38459 E266 A I K S L L P E F M G D R A Q
Baker's Yeast Sacchar. cerevisiae Q12380 294 33541 D235 T L K D I E G D I L D V K E G
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 86.1 86.9 99.2 N.A. 96.7 95.2 N.A. 72.3 89.8 76.4 80.3 N.A. 48 53.8 N.A. 48
Protein Similarity: 100 86.1 88.3 99.6 N.A. 99.2 98.5 N.A. 76 96.7 83.3 90.5 N.A. 68 72.3 N.A. 66.5
P-Site Identity: 100 6.6 0 93.3 N.A. 80 80 N.A. 0 86.6 46.6 53.3 N.A. 13.3 20 N.A. 0
P-Site Similarity: 100 20 26.6 93.3 N.A. 93.3 93.3 N.A. 0 93.3 80 73.3 N.A. 26.6 33.3 N.A. 13.3
Percent
Protein Identity: N.A. N.A. N.A. 27 22.7 N.A.
Protein Similarity: N.A. N.A. N.A. 47.1 40.1 N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 26.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 33.3 60 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 0 14 0 0 0 0 14 47 7 27 7 7 14 0 % A
% Cys: 0 0 0 0 0 40 0 0 0 0 0 0 0 0 7 % C
% Asp: 7 0 0 27 0 7 0 7 7 0 20 20 0 40 0 % D
% Glu: 0 0 7 47 0 14 7 7 0 0 7 0 54 7 7 % E
% Phe: 7 7 0 0 7 14 0 0 14 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 7 0 7 0 0 0 7 0 7 7 47 % G
% His: 0 0 7 0 0 0 0 0 0 0 0 0 0 7 7 % H
% Ile: 7 20 7 0 14 0 7 0 7 27 0 0 7 7 7 % I
% Lys: 0 0 40 7 0 0 7 14 0 0 7 0 7 0 7 % K
% Leu: 60 47 0 7 34 7 0 0 0 7 7 0 7 0 7 % L
% Met: 0 7 0 0 0 0 0 0 0 7 0 7 0 0 0 % M
% Asn: 0 0 0 0 0 7 0 0 7 0 0 7 0 7 0 % N
% Pro: 0 7 0 7 7 0 54 14 0 14 7 34 0 7 0 % P
% Gln: 0 0 7 0 0 0 0 0 7 7 0 0 0 7 7 % Q
% Arg: 7 7 20 0 0 7 7 0 0 7 7 0 7 0 0 % R
% Ser: 0 0 0 7 0 7 0 34 0 0 0 7 0 0 0 % S
% Thr: 7 0 0 0 0 0 14 0 0 0 7 7 0 0 7 % T
% Val: 0 0 0 0 34 0 0 7 14 20 7 14 7 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 7 0 0 0 0 0 7 0 7 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _