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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TPK1 All Species: 21.82
Human Site: T228 Identified Species: 36.92
UniProt: Q9H3S4 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9H3S4 NP_071890.2 243 27265 T228 Y D G S G V V T V E T D H P L
Chimpanzee Pan troglodytes XP_519461 243 27275 T228 Y D G S G V V T V E T D H P L
Rhesus Macaque Macaca mulatta XP_001094591 327 35961 T312 Y D G S G V V T V E T D H P L
Dog Lupus familis XP_532737 208 22899 V194 D G S G V V T V E T D H P L L
Cat Felis silvestris
Mouse Mus musculus Q9R0M5 243 27050 T228 Y D G S G L V T V E T D H P L
Rat Rattus norvegicus NP_001128466 243 27189 T228 Y D G S G L V T V E T D H P L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001506978 182 20341 V168 D G S D D V T V E T D H P L I
Chicken Gallus gallus XP_418875 244 26997 T229 Y D N S G T V T I K T D K P L
Frog Xenopus laevis
Zebra Danio Brachydanio rerio NP_001006074 257 28841 D228 Y E D H D P K D C R K P V T I
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P30636 243 27296 L221 V T T S Q V F L K S S S S L I
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa XP_002302706 255 28531 V240 V Q G E K I T V Q S S S D L L
Maize Zea mays NP_001152100 267 30322 V251 V Q S E K V T V E S D A D L L
Rice Oryza sativa
Thale Cress Arabidopsis thaliana NP_850424 267 30196 V243 V K E E I I T V E S D S D L L
Baker's Yeast Sacchar. cerevisiae P35202 319 36598 F297 F V G D N C C F I D T K D D I
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.5 65.7 76.9 N.A. 89.3 86.8 N.A. 58 73.3 N.A. 59.5 N.A. N.A. N.A. 32 N.A.
Protein Similarity: 100 99.5 69.7 81.8 N.A. 95 93 N.A. 65.8 84.4 N.A. 74.3 N.A. N.A. N.A. 51.8 N.A.
P-Site Identity: 100 100 100 13.3 N.A. 93.3 93.3 N.A. 6.6 66.6 N.A. 6.6 N.A. N.A. N.A. 13.3 N.A.
P-Site Similarity: 100 100 100 13.3 N.A. 100 100 N.A. 13.3 80 N.A. 20 N.A. N.A. N.A. 26.6 N.A.
Percent
Protein Identity: 38.4 36.7 N.A. 36.3 28.5 N.A.
Protein Similarity: 58 57.3 N.A. 55 42.9 N.A.
P-Site Identity: 13.3 13.3 N.A. 6.6 13.3 N.A.
P-Site Similarity: 26.6 13.3 N.A. 13.3 40 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 % A
% Cys: 0 0 0 0 0 8 8 0 8 0 0 0 0 0 0 % C
% Asp: 15 43 8 15 15 0 0 8 0 8 29 43 29 8 0 % D
% Glu: 0 8 8 22 0 0 0 0 29 36 0 0 0 0 0 % E
% Phe: 8 0 0 0 0 0 8 8 0 0 0 0 0 0 0 % F
% Gly: 0 15 50 8 43 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 8 0 0 0 0 0 0 0 15 36 0 0 % H
% Ile: 0 0 0 0 8 15 0 0 15 0 0 0 0 0 29 % I
% Lys: 0 8 0 0 15 0 8 0 8 8 8 8 8 0 0 % K
% Leu: 0 0 0 0 0 15 0 8 0 0 0 0 0 43 72 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 8 0 8 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 8 0 0 0 0 0 8 15 43 0 % P
% Gln: 0 15 0 0 8 0 0 0 8 0 0 0 0 0 0 % Q
% Arg: 0 0 0 0 0 0 0 0 0 8 0 0 0 0 0 % R
% Ser: 0 0 22 50 0 0 0 0 0 29 15 22 8 0 0 % S
% Thr: 0 8 8 0 0 8 36 43 0 15 50 0 0 8 0 % T
% Val: 29 8 0 0 8 50 43 36 36 0 0 0 8 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _