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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NARFL All Species: 25.45
Human Site: T135 Identified Species: 43.08
UniProt: Q9H6Q4 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9H6Q4 NP_071938.1 476 53020 T135 F Q L N P T D T A R K L T S F
Chimpanzee Pan troglodytes XP_510719 476 53016 T135 F Q L N P T D T A R K L T S F
Rhesus Macaque Macaca mulatta XP_001118535 427 47271 Q108 N K M A A P S Q Q R L V V V S
Dog Lupus familis XP_547207 476 52934 T135 F Q L S P T D T A R K L T A F
Cat Felis silvestris
Mouse Mus musculus Q7TMW6 476 53090 T135 F R L D P T D T A R K L T S F
Rat Rattus norvegicus Q5BK18 476 53149 T135 F Q L D S T D T A R K L T S F
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513248 469 52242 L135 S R R L C G F L K S L G V H Y
Chicken Gallus gallus XP_414836 476 53145 T135 C K M S V L E T A K K L T A F
Frog Xenopus laevis Q6GP25 456 51492 D133 L G V H H V L D T T I A A D F
Zebra Danio Brachydanio rerio A2RRV9 411 46081 R92 V F D T A F S R S F S L I E S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q8SYS7 477 54516 A136 Y Q I G V E D A A R H L N G Y
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q9N392 457 50805 F135 A K I I A S F F R R L G V K Y
Sea Urchin Strong. purpuratus XP_001191432 475 53102 T134 Y K L S L P D T A K R L T T F
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7SGW5 581 62269 Q148 C G N G V T E Q Q A G R M I E
Conservation
Percent
Protein Identity: 100 99.5 83.8 86.1 N.A. 87.6 86.1 N.A. 47.9 71.8 44.7 57.1 N.A. 48.6 N.A. 35.9 63.2
Protein Similarity: 100 100 85.9 93.9 N.A. 93.9 93 N.A. 65.3 85.5 64.2 70.1 N.A. 67.7 N.A. 55.6 79.6
P-Site Identity: 100 100 6.6 86.6 N.A. 86.6 86.6 N.A. 0 40 6.6 6.6 N.A. 33.3 N.A. 6.6 46.6
P-Site Similarity: 100 100 26.6 100 N.A. 100 93.3 N.A. 13.3 80 20 13.3 N.A. 53.3 N.A. 33.3 86.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. 32.7
Protein Similarity: N.A. N.A. N.A. N.A. N.A. 47.1
P-Site Identity: N.A. N.A. N.A. N.A. N.A. 6.6
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. 13.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 0 0 8 22 0 0 8 58 8 0 8 8 15 0 % A
% Cys: 15 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 8 15 0 0 50 8 0 0 0 0 0 8 0 % D
% Glu: 0 0 0 0 0 8 15 0 0 0 0 0 0 8 8 % E
% Phe: 36 8 0 0 0 8 15 8 0 8 0 0 0 0 58 % F
% Gly: 0 15 0 15 0 8 0 0 0 0 8 15 0 8 0 % G
% His: 0 0 0 8 8 0 0 0 0 0 8 0 0 8 0 % H
% Ile: 0 0 15 8 0 0 0 0 0 0 8 0 8 8 0 % I
% Lys: 0 29 0 0 0 0 0 0 8 15 43 0 0 8 0 % K
% Leu: 8 0 43 8 8 8 8 8 0 0 22 65 0 0 0 % L
% Met: 0 0 15 0 0 0 0 0 0 0 0 0 8 0 0 % M
% Asn: 8 0 8 15 0 0 0 0 0 0 0 0 8 0 0 % N
% Pro: 0 0 0 0 29 15 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 36 0 0 0 0 0 15 15 0 0 0 0 0 0 % Q
% Arg: 0 15 8 0 0 0 0 8 8 58 8 8 0 0 0 % R
% Ser: 8 0 0 22 8 8 15 0 8 8 8 0 0 29 15 % S
% Thr: 0 0 0 8 0 43 0 50 8 8 0 0 50 8 0 % T
% Val: 8 0 8 0 22 8 0 0 0 0 0 8 22 8 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 15 0 0 0 0 0 0 0 0 0 0 0 0 0 22 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _