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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NO66 All Species: 5.15
Human Site: S249 Identified Species: 10.3
UniProt: Q9H6W3 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0.09
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9H6W3 NP_078920.2 641 71086 S249 F S T A D L D S M L R N E E V
Chimpanzee Pan troglodytes XP_510046 641 71246 S249 F S T A D L D S M L R N E E V
Rhesus Macaque Macaca mulatta XP_001090820 642 71258 L250 F S T A D L D L M L R N E E V
Dog Lupus familis
Cat Felis silvestris
Mouse Mus musculus Q9JJF3 603 67538 G232 D A A R Y V D G R R E T L N P
Rat Rattus norvegicus Q8CFC1 465 53197 R132 R K D F D Q K R A T I Q F H Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001516457 204 22570
Chicken Gallus gallus Q5ZMM1 601 65667 G230 D V T S Y A E G V R E T H N P
Frog Xenopus laevis
Zebra Danio Brachydanio rerio A3KP59 544 61498 V211 P Q A F S S T V W Q V L S V L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q7K4H4 653 73079 E260 I S F K M L D E I L I R H H L
Honey Bee Apis mellifera XP_395039 495 58336 Q162 S V R M L N P Q T Y I P K L H
Nematode Worm Caenorhab. elegans O01658 748 86008 E351 F S T A R L G E L L E K N H L
Sea Urchin Strong. purpuratus XP_782527 555 62864 T222 V G A N I Y L T P P G T Q G F
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.7 95.7 N.A. N.A. 77.8 25.8 N.A. 22.3 49.4 N.A. 45.2 N.A. 32.9 34.1 27.8 38.5
Protein Similarity: 100 99.3 97 N.A. N.A. 83.7 41 N.A. 25.7 61.6 N.A. 61.9 N.A. 50.2 51.1 45.3 55.2
P-Site Identity: 100 100 93.3 N.A. N.A. 6.6 6.6 N.A. 0 6.6 N.A. 0 N.A. 26.6 0 40 0
P-Site Similarity: 100 100 93.3 N.A. N.A. 20 6.6 N.A. 0 26.6 N.A. 6.6 N.A. 40 6.6 53.3 13.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 9 25 34 0 9 0 0 9 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 17 0 9 0 34 0 42 0 0 0 0 0 0 0 0 % D
% Glu: 0 0 0 0 0 0 9 17 0 0 25 0 25 25 0 % E
% Phe: 34 0 9 17 0 0 0 0 0 0 0 0 9 0 9 % F
% Gly: 0 9 0 0 0 0 9 17 0 0 9 0 0 9 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 17 25 9 % H
% Ile: 9 0 0 0 9 0 0 0 9 0 25 0 0 0 0 % I
% Lys: 0 9 0 9 0 0 9 0 0 0 0 9 9 0 0 % K
% Leu: 0 0 0 0 9 42 9 9 9 42 0 9 9 9 25 % L
% Met: 0 0 0 9 9 0 0 0 25 0 0 0 0 0 0 % M
% Asn: 0 0 0 9 0 9 0 0 0 0 0 25 9 17 0 % N
% Pro: 9 0 0 0 0 0 9 0 9 9 0 9 0 0 17 % P
% Gln: 0 9 0 0 0 9 0 9 0 9 0 9 9 0 9 % Q
% Arg: 9 0 9 9 9 0 0 9 9 17 25 9 0 0 0 % R
% Ser: 9 42 0 9 9 9 0 17 0 0 0 0 9 0 0 % S
% Thr: 0 0 42 0 0 0 9 9 9 9 0 25 0 0 0 % T
% Val: 9 17 0 0 0 9 0 9 9 0 9 0 0 9 25 % V
% Trp: 0 0 0 0 0 0 0 0 9 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 17 9 0 0 0 9 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _