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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NO66 All Species: 9.09
Human Site: S325 Identified Species: 18.18
UniProt: Q9H6W3 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0.18
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9H6W3 NP_078920.2 641 71086 S325 Q F G S M A G S N V Y L T P P
Chimpanzee Pan troglodytes XP_510046 641 71246 S325 Q F G S M A G S N V Y L T P P
Rhesus Macaque Macaca mulatta XP_001090820 642 71258 S326 Q F G S M A G S N V Y L T P P
Dog Lupus familis
Cat Felis silvestris
Mouse Mus musculus Q9JJF3 603 67538 I306 F A P H Y D D I E A F V L Q L
Rat Rattus norvegicus Q8CFC1 465 53197 L205 L Y S P T V P L A R E Y S V E
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001516457 204 22570
Chicken Gallus gallus Q5ZMM1 601 65667 I304 F A P H Y D D I E A F V L Q L
Frog Xenopus laevis
Zebra Danio Brachydanio rerio A3KP59 544 61498 E284 S P N F S Q D E I G E P V M D
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q7K4H4 653 73079 A336 F F H C K V G A N L Y L T P P
Honey Bee Apis mellifera XP_395039 495 58336 E235 Y L P R Y S S E N F S Q F E I
Nematode Worm Caenorhab. elegans O01658 748 86008 A427 Q F G C F V G A N T Y L T P A
Sea Urchin Strong. purpuratus XP_782527 555 62864 V295 L L Y F P R G V I H Q A S T P
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.7 95.7 N.A. N.A. 77.8 25.8 N.A. 22.3 49.4 N.A. 45.2 N.A. 32.9 34.1 27.8 38.5
Protein Similarity: 100 99.3 97 N.A. N.A. 83.7 41 N.A. 25.7 61.6 N.A. 61.9 N.A. 50.2 51.1 45.3 55.2
P-Site Identity: 100 100 100 N.A. N.A. 0 0 N.A. 0 0 N.A. 0 N.A. 53.3 6.6 60 13.3
P-Site Similarity: 100 100 100 N.A. N.A. 13.3 13.3 N.A. 0 13.3 N.A. 0 N.A. 66.6 13.3 66.6 20
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 17 0 0 0 25 0 17 9 17 0 9 0 0 9 % A
% Cys: 0 0 0 17 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 17 25 0 0 0 0 0 0 0 9 % D
% Glu: 0 0 0 0 0 0 0 17 17 0 17 0 0 9 9 % E
% Phe: 25 42 0 17 9 0 0 0 0 9 17 0 9 0 0 % F
% Gly: 0 0 34 0 0 0 50 0 0 9 0 0 0 0 0 % G
% His: 0 0 9 17 0 0 0 0 0 9 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 17 17 0 0 0 0 0 9 % I
% Lys: 0 0 0 0 9 0 0 0 0 0 0 0 0 0 0 % K
% Leu: 17 17 0 0 0 0 0 9 0 9 0 42 17 0 17 % L
% Met: 0 0 0 0 25 0 0 0 0 0 0 0 0 9 0 % M
% Asn: 0 0 9 0 0 0 0 0 50 0 0 0 0 0 0 % N
% Pro: 0 9 25 9 9 0 9 0 0 0 0 9 0 42 42 % P
% Gln: 34 0 0 0 0 9 0 0 0 0 9 9 0 17 0 % Q
% Arg: 0 0 0 9 0 9 0 0 0 9 0 0 0 0 0 % R
% Ser: 9 0 9 25 9 9 9 25 0 0 9 0 17 0 0 % S
% Thr: 0 0 0 0 9 0 0 0 0 9 0 0 42 9 0 % T
% Val: 0 0 0 0 0 25 0 9 0 25 0 17 9 9 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 9 9 9 0 25 0 0 0 0 0 42 9 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _