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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MED4 All Species: 52.12
Human Site: S145 Identified Species: 95.56
UniProt: Q9NPJ6 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9NPJ6 NP_054885.1 270 29745 S145 A R K G A I S S E E I I K Y A
Chimpanzee Pan troglodytes XP_001151408 248 28027 S123 A R K G A I S S E E I I K Y A
Rhesus Macaque Macaca mulatta XP_001100111 270 29757 S145 A R K G A I S S E E I I K Y A
Dog Lupus familis XP_534120 363 39725 S238 A R K G A I S S E E I I K Y A
Cat Felis silvestris
Mouse Mus musculus Q9CQA5 270 29763 S145 A R K G A I S S E E I I K Y A
Rat Rattus norvegicus Q561Q8 270 29807 S145 A R K G A I S S E E I I K Y A
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514128 248 27969 S123 A R K G A I S S E E I I K Y A
Chicken Gallus gallus XP_417054 261 28661 S136 A R K G A I S S E E I I K Y A
Frog Xenopus laevis Q5XG48 252 28037 S127 A R K G V I S S E E L I K Y A
Zebra Danio Brachydanio rerio Q561X3 254 28034 S129 A R K G S I S S K E I I K Y A
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q9VS38 258 28294 S121 A N K R P V S S E E L I K Y A
Honey Bee Apis mellifera XP_396963 255 28162 S126 A N K R P V P S E E L I K Y A
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_781304 249 27006 S124 A K K G A I S S E E L I K Y A
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 86.6 99.6 71.6 N.A. 94.4 93.6 N.A. 82.2 86.3 78.8 77.4 N.A. 41.8 50 N.A. 47.4
Protein Similarity: 100 87.4 99.6 73.2 N.A. 97.4 97 N.A. 88.1 93.3 85.5 85.5 N.A. 59.6 67.4 N.A. 65.1
P-Site Identity: 100 100 100 100 N.A. 100 100 N.A. 100 100 86.6 86.6 N.A. 66.6 60 N.A. 86.6
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. 100 100 93.3 100 N.A. 80 73.3 N.A. 100
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 100 0 0 0 70 0 0 0 0 0 0 0 0 0 100 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % D
% Glu: 0 0 0 0 0 0 0 0 93 100 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 85 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 85 0 0 0 0 70 100 0 0 0 % I
% Lys: 0 8 100 0 0 0 0 0 8 0 0 0 100 0 0 % K
% Leu: 0 0 0 0 0 0 0 0 0 0 31 0 0 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 16 0 0 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 16 0 8 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 77 0 16 0 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 0 0 0 0 8 0 93 100 0 0 0 0 0 0 0 % S
% Thr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % T
% Val: 0 0 0 0 8 16 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _