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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: SPAST All Species: 25.15
Human Site: T255 Identified Species: 42.56
UniProt: Q9UBP0 Number Species: 13
    Phosphosite Substitution
    Charge Score: -0.31
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9UBP0 NP_055761.2 616 67197 T255 R S K T V M K T G S A G L S G
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta
Dog Lupus familis XP_850973 624 68082 T263 R S K T V M K T G S T G L S G
Cat Felis silvestris
Mouse Mus musculus Q9QYY8 614 66438 S253 R S K T V L K S G S A G L S G
Rat Rattus norvegicus B2RYN7 581 63003 S220 R S K T V M K S G S T G L S G
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509194 572 63669 T211 R S K A V A K T G S A G L S G
Chicken Gallus gallus Q5ZK92 613 66247 T252 R S K T V A K T G S T G L S G
Frog Xenopus laevis Q6AZT2 600 65835 T239 T S N S Y S R T K A P P K S G
Zebra Danio Brachydanio rerio Q6NW58 570 63067 R208 I T N Q T S L R P K N P P K S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q8I0P1 758 82731 P396 A N K S Q T L P R N L G S K T
Honey Bee Apis mellifera XP_393080 682 76301 R321 S K S Q T L P R S M G R S T A
Nematode Worm Caenorhab. elegans Q8MNV0 512 56883 S185 P E P P V Q A S N R K M E T V
Sea Urchin Strong. purpuratus O61577 516 57575 K189 K D K N R G G K S D K D K K A
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9SEX2 523 57218 K196 G A A S K S N K A E S M N G D
Baker's Yeast Sacchar. cerevisiae P39955 897 100313 S470 A L N T T K K S H P I L K S K
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 N.A. N.A. 94.7 N.A. 92.5 88.9 N.A. 81.1 83.9 71 62.8 N.A. 42.8 46.9 33.7 30.3
Protein Similarity: 100 N.A. N.A. 95.8 N.A. 93.9 90 N.A. 84.4 88.4 80.8 73.3 N.A. 55.2 62 49.3 46.7
P-Site Identity: 100 N.A. N.A. 93.3 N.A. 86.6 86.6 N.A. 86.6 86.6 26.6 0 N.A. 13.3 0 6.6 6.6
P-Site Similarity: 100 N.A. N.A. 93.3 N.A. 100 93.3 N.A. 86.6 86.6 46.6 6.6 N.A. 33.3 13.3 20 13.3
Percent
Protein Identity: N.A. N.A. N.A. 31 30.1 N.A.
Protein Similarity: N.A. N.A. N.A. 49.3 45.8 N.A.
P-Site Identity: N.A. N.A. N.A. 0 20 N.A.
P-Site Similarity: N.A. N.A. N.A. 20 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 15 8 8 8 0 15 8 0 8 8 22 0 0 0 15 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 8 0 0 0 0 0 0 0 8 0 8 0 0 8 % D
% Glu: 0 8 0 0 0 0 0 0 0 8 0 0 8 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 8 0 0 0 0 8 8 0 43 0 8 50 0 8 50 % G
% His: 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 % H
% Ile: 8 0 0 0 0 0 0 0 0 0 8 0 0 0 0 % I
% Lys: 8 8 58 0 8 8 50 15 8 8 15 0 22 22 8 % K
% Leu: 0 8 0 0 0 15 15 0 0 0 8 8 43 0 0 % L
% Met: 0 0 0 0 0 22 0 0 0 8 0 15 0 0 0 % M
% Asn: 0 8 22 8 0 0 8 0 8 8 8 0 8 0 0 % N
% Pro: 8 0 8 8 0 0 8 8 8 8 8 15 8 0 0 % P
% Gln: 0 0 0 15 8 8 0 0 0 0 0 0 0 0 0 % Q
% Arg: 43 0 0 0 8 0 8 15 8 8 0 8 0 0 0 % R
% Ser: 8 50 8 22 0 22 0 29 15 43 8 0 15 58 8 % S
% Thr: 8 8 0 43 22 8 0 36 0 0 22 0 0 15 8 % T
% Val: 0 0 0 0 50 0 0 0 0 0 0 0 0 0 8 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _