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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NENF All Species: 27.58
Human Site: Y65 Identified Species: 50.56
UniProt: Q9UMX5 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9UMX5 NP_037481.1 172 18856 Y65 E E E D Q P I Y L A V K G V V
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta XP_001108201 172 18711 Y65 E E E D Q P I Y L A V K G V V
Dog Lupus familis XP_537145 151 16532 K48 Q P I Y M A V K G V V F D V T
Cat Felis silvestris
Mouse Mus musculus Q9CQ45 171 18886 Y64 E E E D Q P I Y L A V K G V V
Rat Rattus norvegicus Q6IUR5 171 18973 Y64 E E E D Q P I Y L A V K G V V
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509736 165 18361 Y58 K E E D Q P I Y L A V K G V V
Chicken Gallus gallus Q5ZKN2 192 21263 L88 G V R D P R I L M A V N G K V
Frog Xenopus laevis
Zebra Danio Brachydanio rerio A2CES0 267 29696 Y72 G K N S K G L Y L A I L G Q V
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q9W376 287 32609 Y82 E E E G R P L Y L A L L G S V
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q9XXA7 326 36224 Y119 T R D S K P I Y L A I L G R V
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa XP_002324923 100 10911
Maize Zea mays NP_001149328 232 24549 L89 S D P K K P L L M A I K G Q I
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9SK39 100 11013
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 N.A. 98.8 70.3 N.A. 90.6 88.3 N.A. 77.3 28.6 N.A. 21.7 N.A. 25 N.A. 22.3 N.A.
Protein Similarity: 100 N.A. 98.8 75.5 N.A. 93.5 92.4 N.A. 84.8 44.7 N.A. 34.8 N.A. 36.5 N.A. 31.8 N.A.
P-Site Identity: 100 N.A. 100 13.3 N.A. 100 100 N.A. 93.3 40 N.A. 33.3 N.A. 60 N.A. 46.6 N.A.
P-Site Similarity: 100 N.A. 100 26.6 N.A. 100 100 N.A. 100 46.6 N.A. 60 N.A. 80 N.A. 66.6 N.A.
Percent
Protein Identity: 29 30.6 N.A. 27.9 N.A. N.A.
Protein Similarity: 39.5 43.5 N.A. 41.8 N.A. N.A.
P-Site Identity: 0 26.6 N.A. 0 N.A. N.A.
P-Site Similarity: 0 66.6 N.A. 0 N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 8 0 0 0 77 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 8 8 47 0 0 0 0 0 0 0 0 8 0 0 % D
% Glu: 39 47 47 0 0 0 0 0 0 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 % F
% Gly: 16 0 0 8 0 8 0 0 8 0 0 0 77 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 8 0 0 0 54 0 0 0 24 0 0 0 8 % I
% Lys: 8 8 0 8 24 0 0 8 0 0 0 47 0 8 0 % K
% Leu: 0 0 0 0 0 0 24 16 62 0 8 24 0 0 0 % L
% Met: 0 0 0 0 8 0 0 0 16 0 0 0 0 0 0 % M
% Asn: 0 0 8 0 0 0 0 0 0 0 0 8 0 0 0 % N
% Pro: 0 8 8 0 8 62 0 0 0 0 0 0 0 0 0 % P
% Gln: 8 0 0 0 39 0 0 0 0 0 0 0 0 16 0 % Q
% Arg: 0 8 8 0 8 8 0 0 0 0 0 0 0 8 0 % R
% Ser: 8 0 0 16 0 0 0 0 0 0 0 0 0 8 0 % S
% Thr: 8 0 0 0 0 0 0 0 0 0 0 0 0 0 8 % T
% Val: 0 8 0 0 0 0 8 0 0 8 54 0 0 47 70 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 8 0 0 0 62 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _