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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: DOLK All Species: 16.67
Human Site: S325 Identified Species: 40.74
UniProt: Q9UPQ8 Number Species: 9
    Phosphosite Substitution
    Charge Score: -0.22
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9UPQ8 NP_055723.1 538 59268 S325 Q N A K R S S S E S K K H Q A
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta
Dog Lupus familis XP_537820 536 59260 S323 Q N A K R S S S E S K K H Q A
Cat Felis silvestris
Mouse Mus musculus Q8R2Y3 534 59127 S321 Q N A K R S S S E S K K H R A
Rat Rattus norvegicus NP_001101296 536 59200 S323 Q N A K R S S S E S K K H K A
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001517971 485 52832 R273 V W L Q N S K R S S A E S K K
Chicken Gallus gallus XP_415482 523 58204 S310 Y Q N A K R S S E S K K H Q A
Frog Xenopus laevis NP_001086008 521 57639 R308 V L Y Q N S K R S P D S K K L
Zebra Danio Brachydanio rerio
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa XP_002313531 573 64284 L357 C V I Y L S V L R F Y N I S K
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana NP_190090 569 63118 V353 Y W I L L I V V S V S R F Y N
Baker's Yeast Sacchar. cerevisiae P20048 519 58888 L302 S T I R Q K I L F A W S S I L
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 N.A. N.A. 94.2 N.A. 94.8 94.9 N.A. 69.6 76.2 69.8 N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: 100 N.A. N.A. 96.4 N.A. 96.4 96.4 N.A. 79.3 87.1 83.4 N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: 100 N.A. N.A. 100 N.A. 93.3 93.3 N.A. 13.3 60 6.6 N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: 100 N.A. N.A. 100 N.A. 100 100 N.A. 33.3 66.6 20 N.A. N.A. N.A. N.A. N.A. N.A.
Percent
Protein Identity: 24.6 N.A. N.A. 26.8 23.4 N.A.
Protein Similarity: 45.2 N.A. N.A. 46 44.8 N.A.
P-Site Identity: 6.6 N.A. N.A. 0 0 N.A.
P-Site Similarity: 6.6 N.A. N.A. 6.6 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 40 10 0 0 0 0 0 10 10 0 0 0 50 % A
% Cys: 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 0 10 0 0 0 0 % D
% Glu: 0 0 0 0 0 0 0 0 50 0 0 10 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 10 10 0 0 10 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 50 0 0 % H
% Ile: 0 0 30 0 0 10 10 0 0 0 0 0 10 10 0 % I
% Lys: 0 0 0 40 10 10 20 0 0 0 50 50 10 30 20 % K
% Leu: 0 10 10 10 20 0 0 20 0 0 0 0 0 0 20 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 40 10 0 20 0 0 0 0 0 0 10 0 0 10 % N
% Pro: 0 0 0 0 0 0 0 0 0 10 0 0 0 0 0 % P
% Gln: 40 10 0 20 10 0 0 0 0 0 0 0 0 30 0 % Q
% Arg: 0 0 0 10 40 10 0 20 10 0 0 10 0 10 0 % R
% Ser: 10 0 0 0 0 70 50 50 30 60 10 20 20 10 0 % S
% Thr: 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 % T
% Val: 20 10 0 0 0 0 20 10 0 10 0 0 0 0 0 % V
% Trp: 0 20 0 0 0 0 0 0 0 0 10 0 0 0 0 % W
% Tyr: 20 0 10 10 0 0 0 0 0 0 10 0 0 10 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _