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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ASF1A All Species: 10.61
Human Site: S133 Identified Species: 16.67
UniProt: Q9Y294 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.36
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9Y294 NP_054753.1 204 22969 S133 P P V K P D F S K L Q R N I L
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta XP_001111289 202 22429 L122 N E Y L N P E L R E N P P M K
Dog Lupus familis XP_542021 201 22312 L122 N E Y P N P E L R E N P P L K
Cat Felis silvestris
Mouse Mus musculus Q9CQE6 204 22924 S133 P P V K P D F S K L Q R N I L
Rat Rattus norvegicus NP_001099859 204 22882 S133 P P V K P D F S K L Q R N I L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001511928 201 22195 L122 N E Y T D P E L R E N P P L K
Chicken Gallus gallus
Frog Xenopus laevis Q69DB9 201 22818 P130 R E N P P V K P D F S K L Q R
Zebra Danio Brachydanio rerio Q6NY34 197 22036 P127 P E L R E N P P V K P D Y T Q
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q9V464 218 24391 E133 P P T K P L F E K L T R N I L
Honey Bee Apis mellifera XP_624816 198 22392 L122 N E Y T D A E L R E N P P P Q
Nematode Worm Caenorhab. elegans Q17603 245 27978 E134 P P A K P L I E K L S R K I E
Sea Urchin Strong. purpuratus XP_793879 221 24848 T133 N E S E K Q P T V P Q F D K V
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9LS09 218 24682 D133 P P T K V L I D K V Q R N I L
Baker's Yeast Sacchar. cerevisiae P32447 279 31585 D133 P P A K V Q V D H I V R N I L
Red Bread Mold Neurospora crassa Q7S1X9 271 30161 E133 P P P K P V I E K I R R N V L
Conservation
Percent
Protein Identity: 100 N.A. 71.5 70.5 N.A. 99.5 98 N.A. 72 N.A. 92.6 74.5 N.A. 57.3 59.7 40.4 58.3
Protein Similarity: 100 N.A. 82.3 80.3 N.A. 99.5 99.5 N.A. 85.2 N.A. 95.5 84.8 N.A. 72.4 73 55 68.3
P-Site Identity: 100 N.A. 0 0 N.A. 100 100 N.A. 0 N.A. 6.6 6.6 N.A. 73.3 0 53.3 6.6
P-Site Similarity: 100 N.A. 13.3 13.3 N.A. 100 100 N.A. 13.3 N.A. 13.3 26.6 N.A. 73.3 6.6 53.3 33.3
Percent
Protein Identity: N.A. N.A. N.A. 48.1 37.6 38.3
Protein Similarity: N.A. N.A. N.A. 66 52.6 54.9
P-Site Identity: N.A. N.A. N.A. 60 46.6 53.3
P-Site Similarity: N.A. N.A. N.A. 66.6 53.3 73.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 14 0 0 7 0 0 0 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 14 20 0 14 7 0 0 7 7 0 0 % D
% Glu: 0 47 0 7 7 0 27 20 0 27 0 0 0 0 7 % E
% Phe: 0 0 0 0 0 0 27 0 0 7 0 7 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 20 0 0 14 0 0 0 47 0 % I
% Lys: 0 0 0 54 7 0 7 0 47 7 0 7 7 7 20 % K
% Leu: 0 0 7 7 0 20 0 27 0 34 0 0 7 14 47 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 % M
% Asn: 34 0 7 0 14 7 0 0 0 0 27 0 47 0 0 % N
% Pro: 60 54 7 14 47 20 14 14 0 7 7 27 27 7 0 % P
% Gln: 0 0 0 0 0 14 0 0 0 0 34 0 0 7 14 % Q
% Arg: 7 0 0 7 0 0 0 0 27 0 7 54 0 0 7 % R
% Ser: 0 0 7 0 0 0 0 20 0 0 14 0 0 0 0 % S
% Thr: 0 0 14 14 0 0 0 7 0 0 7 0 0 7 0 % T
% Val: 0 0 20 0 14 14 7 0 14 7 7 0 0 7 7 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 27 0 0 0 0 0 0 0 0 0 7 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _