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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
ATG4B
All Species:
8.18
Human Site:
S199
Identified Species:
13.85
UniProt:
Q9Y4P1
Number Species:
13
Phosphosite Substitution
Charge Score:
0.23
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
Q9Y4P1
NP_037457.3
393
44309
S199
A
T
A
F
P
A
D
S
D
R
H
C
N
G
F
Chimpanzee
Pan troglodytes
XP_001162556
306
34202
A116
C
R
T
S
V
P
C
A
G
A
T
A
F
P
A
Rhesus Macaque
Macaca mulatta
XP_001097251
398
45435
L201
D
T
A
G
D
R
P
L
D
Y
L
T
A
S
N
Dog
Lupus familis
XP_851977
394
44349
S200
A
A
A
L
P
A
D
S
S
R
H
C
N
G
F
Cat
Felis silvestris
Mouse
Mus musculus
Q8BGE6
393
44357
S199
A
A
A
L
P
T
D
S
E
R
H
C
N
G
F
Rat
Rattus norvegicus
NP_001119770
406
45887
P202
D
T
A
G
E
S
P
P
D
S
L
I
A
S
S
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
XP_001513122
528
59425
E335
S
A
F
P
T
D
S
E
F
L
L
N
G
F
P
Chicken
Gallus gallus
Q6PZ02
393
44512
E198
A
A
A
C
P
A
V
E
A
D
V
L
Y
N
G
Frog
Xenopus laevis
Q640G7
384
43139
S190
R
A
G
S
G
E
S
S
D
A
G
A
L
S
N
Zebra Danio
Brachydanio rerio
Q6DG88
394
44435
E199
G
A
C
A
V
S
E
E
P
R
E
M
N
G
D
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
Honey Bee
Apis mellifera
XP_624577
382
43001
G186
K
Q
C
R
V
E
G
G
T
T
V
E
A
D
G
Nematode Worm
Caenorhab. elegans
Sea Urchin
Strong. purpuratus
XP_001198708
393
44309
G202
T
E
E
T
S
S
E
G
S
K
T
G
S
E
R
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Q8S929
467
51510
H221
E
P
S
A
F
S
I
H
N
L
I
I
A
G
A
Baker's Yeast
Sacchar. cerevisiae
Red Bread Mold
Neurospora crassa
Q7S3X7
506
55337
Y288
G
A
T
A
C
G
K
Y
P
G
E
W
F
G
P
Conservation
Percent
Protein Identity:
100
74.8
52.5
94.1
N.A.
94.9
52.4
N.A.
65.1
86
73.7
72.8
N.A.
N.A.
49.1
N.A.
49.8
Protein Similarity:
100
75.5
72.6
96.1
N.A.
96.6
70.9
N.A.
70
92.3
87
83.2
N.A.
N.A.
64.8
N.A.
64.3
P-Site Identity:
100
0
20
80
N.A.
73.3
20
N.A.
0
26.6
13.3
20
N.A.
N.A.
0
N.A.
0
P-Site Similarity:
100
6.6
20
80
N.A.
80
26.6
N.A.
6.6
26.6
13.3
33.3
N.A.
N.A.
0
N.A.
26.6
Percent
Protein Identity:
N.A.
N.A.
N.A.
23.1
N.A.
23.5
Protein Similarity:
N.A.
N.A.
N.A.
39.8
N.A.
38.3
P-Site Identity:
N.A.
N.A.
N.A.
6.6
N.A.
6.6
P-Site Similarity:
N.A.
N.A.
N.A.
26.6
N.A.
6.6
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
29
50
43
22
0
22
0
8
8
15
0
15
29
0
15
% A
% Cys:
8
0
15
8
8
0
8
0
0
0
0
22
0
0
0
% C
% Asp:
15
0
0
0
8
8
22
0
29
8
0
0
0
8
8
% D
% Glu:
8
8
8
0
8
15
15
22
8
0
15
8
0
8
0
% E
% Phe:
0
0
8
8
8
0
0
0
8
0
0
0
15
8
22
% F
% Gly:
15
0
8
15
8
8
8
15
8
8
8
8
8
43
15
% G
% His:
0
0
0
0
0
0
0
8
0
0
22
0
0
0
0
% H
% Ile:
0
0
0
0
0
0
8
0
0
0
8
15
0
0
0
% I
% Lys:
8
0
0
0
0
0
8
0
0
8
0
0
0
0
0
% K
% Leu:
0
0
0
15
0
0
0
8
0
15
22
8
8
0
0
% L
% Met:
0
0
0
0
0
0
0
0
0
0
0
8
0
0
0
% M
% Asn:
0
0
0
0
0
0
0
0
8
0
0
8
29
8
15
% N
% Pro:
0
8
0
8
29
8
15
8
15
0
0
0
0
8
15
% P
% Gln:
0
8
0
0
0
0
0
0
0
0
0
0
0
0
0
% Q
% Arg:
8
8
0
8
0
8
0
0
0
29
0
0
0
0
8
% R
% Ser:
8
0
8
15
8
29
15
29
15
8
0
0
8
22
8
% S
% Thr:
8
22
15
8
8
8
0
0
8
8
15
8
0
0
0
% T
% Val:
0
0
0
0
22
0
8
0
0
0
15
0
0
0
0
% V
% Trp:
0
0
0
0
0
0
0
0
0
0
0
8
0
0
0
% W
% Tyr:
0
0
0
0
0
0
0
8
0
8
0
0
8
0
0
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _