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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
ATG4B
All Species:
36.36
Human Site:
S316
Identified Species:
61.54
UniProt:
Q9Y4P1
Number Species:
13
Phosphosite Substitution
Charge Score:
0.08
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
Q9Y4P1
NP_037457.3
393
44309
S316
S
I
A
E
L
D
P
S
I
A
V
G
F
F
C
Chimpanzee
Pan troglodytes
XP_001162556
306
34202
C232
F
H
C
Q
H
P
P
C
R
M
S
I
A
E
L
Rhesus Macaque
Macaca mulatta
XP_001097251
398
45435
S317
N
I
L
N
L
D
P
S
V
A
L
G
F
F
C
Dog
Lupus familis
XP_851977
394
44349
S317
S
I
G
E
L
D
P
S
I
A
V
G
F
F
C
Cat
Felis silvestris
Mouse
Mus musculus
Q8BGE6
393
44357
S316
G
I
G
E
L
D
P
S
I
A
V
G
F
F
C
Rat
Rattus norvegicus
NP_001119770
406
45887
S318
S
I
L
N
L
D
P
S
V
A
L
V
G
Q
G
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
XP_001513122
528
59425
S451
N
I
V
E
L
D
P
S
I
A
V
G
F
F
C
Chicken
Gallus gallus
Q6PZ02
393
44512
S316
S
I
A
E
L
D
P
S
I
A
V
G
F
F
C
Frog
Xenopus laevis
Q640G7
384
43139
S307
H
V
S
E
I
D
P
S
I
A
V
G
F
F
C
Zebra Danio
Brachydanio rerio
Q6DG88
394
44435
S318
H
I
C
E
L
D
P
S
I
A
A
G
F
F
C
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
Honey Bee
Apis mellifera
XP_624577
382
43001
S303
P
I
I
E
I
D
P
S
V
A
L
C
F
F
C
Nematode Worm
Caenorhab. elegans
Sea Urchin
Strong. purpuratus
XP_001198708
393
44309
N319
A
S
R
M
P
I
K
N
L
D
P
S
I
G
L
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Q8S929
467
51510
S338
A
T
F
T
F
P
Q
S
V
G
I
L
G
G
K
Baker's Yeast
Sacchar. cerevisiae
Red Bread Mold
Neurospora crassa
Q7S3X7
506
55337
E411
R
P
A
L
P
Y
R
E
D
P
R
G
Y
T
A
Conservation
Percent
Protein Identity:
100
74.8
52.5
94.1
N.A.
94.9
52.4
N.A.
65.1
86
73.7
72.8
N.A.
N.A.
49.1
N.A.
49.8
Protein Similarity:
100
75.5
72.6
96.1
N.A.
96.6
70.9
N.A.
70
92.3
87
83.2
N.A.
N.A.
64.8
N.A.
64.3
P-Site Identity:
100
6.6
66.6
93.3
N.A.
86.6
46.6
N.A.
86.6
100
73.3
80
N.A.
N.A.
60
N.A.
0
P-Site Similarity:
100
13.3
86.6
93.3
N.A.
86.6
60
N.A.
93.3
100
93.3
80
N.A.
N.A.
80
N.A.
20
Percent
Protein Identity:
N.A.
N.A.
N.A.
23.1
N.A.
23.5
Protein Similarity:
N.A.
N.A.
N.A.
39.8
N.A.
38.3
P-Site Identity:
N.A.
N.A.
N.A.
6.6
N.A.
13.3
P-Site Similarity:
N.A.
N.A.
N.A.
26.6
N.A.
20
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
15
0
22
0
0
0
0
0
0
72
8
0
8
0
8
% A
% Cys:
0
0
15
0
0
0
0
8
0
0
0
8
0
0
65
% C
% Asp:
0
0
0
0
0
72
0
0
8
8
0
0
0
0
0
% D
% Glu:
0
0
0
58
0
0
0
8
0
0
0
0
0
8
0
% E
% Phe:
8
0
8
0
8
0
0
0
0
0
0
0
65
65
0
% F
% Gly:
8
0
15
0
0
0
0
0
0
8
0
65
15
15
8
% G
% His:
15
8
0
0
8
0
0
0
0
0
0
0
0
0
0
% H
% Ile:
0
65
8
0
15
8
0
0
50
0
8
8
8
0
0
% I
% Lys:
0
0
0
0
0
0
8
0
0
0
0
0
0
0
8
% K
% Leu:
0
0
15
8
58
0
0
0
8
0
22
8
0
0
15
% L
% Met:
0
0
0
8
0
0
0
0
0
8
0
0
0
0
0
% M
% Asn:
15
0
0
15
0
0
0
8
0
0
0
0
0
0
0
% N
% Pro:
8
8
0
0
15
15
79
0
0
8
8
0
0
0
0
% P
% Gln:
0
0
0
8
0
0
8
0
0
0
0
0
0
8
0
% Q
% Arg:
8
0
8
0
0
0
8
0
8
0
8
0
0
0
0
% R
% Ser:
29
8
8
0
0
0
0
79
0
0
8
8
0
0
0
% S
% Thr:
0
8
0
8
0
0
0
0
0
0
0
0
0
8
0
% T
% Val:
0
8
8
0
0
0
0
0
29
0
43
8
0
0
0
% V
% Trp:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% W
% Tyr:
0
0
0
0
0
8
0
0
0
0
0
0
8
0
0
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _