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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4B All Species: 24.85
Human Site: S372 Identified Species: 42.05
UniProt: Q9Y4P1 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9Y4P1 NP_037457.3 393 44309 S372 L N L S L D S S D V E R L E R
Chimpanzee Pan troglodytes XP_001162556 306 34202 A286 E Q Q P S H L A C P D V L N L
Rhesus Macaque Macaca mulatta XP_001097251 398 45435 E376 E V T T T G A E F I D S T E Q
Dog Lupus familis XP_851977 394 44349 S373 L N L S L D S S D V E R L E R
Cat Felis silvestris
Mouse Mus musculus Q8BGE6 393 44357 S372 L N L S L D S S D V E R L E R
Rat Rattus norvegicus NP_001119770 406 45887 E384 E V T T T G A E F I D S T E Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513122 528 59425 S507 L N L S L D S S D A E R L E R
Chicken Gallus gallus Q6PZ02 393 44512 S372 L N L T P D S S D A D R L E R
Frog Xenopus laevis Q640G7 384 43139 S363 L N L T P D S S D A D R L D R
Zebra Danio Brachydanio rerio Q6DG88 394 44435 S373 L N L T P D F S D S D R L E R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_624577 382 43001 V362 A I A A S S F V D F E H V D P
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001198708 393 44309 R373 I E P S F E I R D C E Y P D E
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 L399 R Y V P L E S L D P S L A L G
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 P476 I S V S P Y D P A R G Q G G G
Conservation
Percent
Protein Identity: 100 74.8 52.5 94.1 N.A. 94.9 52.4 N.A. 65.1 86 73.7 72.8 N.A. N.A. 49.1 N.A. 49.8
Protein Similarity: 100 75.5 72.6 96.1 N.A. 96.6 70.9 N.A. 70 92.3 87 83.2 N.A. N.A. 64.8 N.A. 64.3
P-Site Identity: 100 6.6 6.6 100 N.A. 100 6.6 N.A. 93.3 73.3 66.6 66.6 N.A. N.A. 13.3 N.A. 20
P-Site Similarity: 100 20 40 100 N.A. 100 40 N.A. 93.3 86.6 86.6 80 N.A. N.A. 33.3 N.A. 40
Percent
Protein Identity: N.A. N.A. N.A. 23.1 N.A. 23.5
Protein Similarity: N.A. N.A. N.A. 39.8 N.A. 38.3
P-Site Identity: N.A. N.A. N.A. 20 N.A. 6.6
P-Site Similarity: N.A. N.A. N.A. 33.3 N.A. 33.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 0 8 8 0 0 15 8 8 22 0 0 8 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 8 8 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 50 8 0 72 0 43 0 0 22 0 % D
% Glu: 22 8 0 0 0 15 0 15 0 0 43 0 0 58 8 % E
% Phe: 0 0 0 0 8 0 15 0 15 8 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 15 0 0 0 0 8 0 8 8 15 % G
% His: 0 0 0 0 0 8 0 0 0 0 0 8 0 0 0 % H
% Ile: 15 8 0 0 0 0 8 0 0 15 0 0 0 0 0 % I
% Lys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % K
% Leu: 50 0 50 0 36 0 8 8 0 0 0 8 58 8 8 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 50 0 0 0 0 0 0 0 0 0 0 0 8 0 % N
% Pro: 0 0 8 15 29 0 0 8 0 15 0 0 8 0 8 % P
% Gln: 0 8 8 0 0 0 0 0 0 0 0 8 0 0 15 % Q
% Arg: 8 0 0 0 0 0 0 8 0 8 0 50 0 0 50 % R
% Ser: 0 8 0 43 15 8 50 50 0 8 8 15 0 0 0 % S
% Thr: 0 0 15 36 15 0 0 0 0 0 0 0 15 0 0 % T
% Val: 0 15 15 0 0 0 0 8 0 22 0 8 8 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 8 0 0 0 8 0 0 0 0 0 8 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _