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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4B All Species: 21.82
Human Site: S44 Identified Species: 36.92
UniProt: Q9Y4P1 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9Y4P1 NP_037457.3 393 44309 S44 T E K D E I L S D V A S R L W
Chimpanzee Pan troglodytes XP_001162556 306 34202
Rhesus Macaque Macaca mulatta XP_001097251 398 45435 S47 T E K S K L L S D I S A R L W
Dog Lupus familis XP_851977 394 44349 S45 T E K D E I L S D V A S R L W
Cat Felis silvestris
Mouse Mus musculus Q8BGE6 393 44357 S44 T E K D E I L S D V A S R L W
Rat Rattus norvegicus NP_001119770 406 45887 S47 T E K S K L L S D I S A R L W
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513122 528 59425 S180 T E K E D I L S D V T S R L W
Chicken Gallus gallus Q6PZ02 393 44512 D44 E K E E I L L D V T S R L W F
Frog Xenopus laevis Q640G7 384 43139 N44 T E K E Q L L N D I T S R L W
Zebra Danio Brachydanio rerio Q6DG88 394 44435 A44 T E K D D I L A D V T S R L W
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_624577 382 43001 R44 D A I R R D I R S K L W F T Y
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001198708 393 44309 L51 Q H Q L E A R L D V L S R L W
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 F47 F T L W S N V F T S S S S V S
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 L83 I K Q Y G S P L S S S S L S Q
Conservation
Percent
Protein Identity: 100 74.8 52.5 94.1 N.A. 94.9 52.4 N.A. 65.1 86 73.7 72.8 N.A. N.A. 49.1 N.A. 49.8
Protein Similarity: 100 75.5 72.6 96.1 N.A. 96.6 70.9 N.A. 70 92.3 87 83.2 N.A. N.A. 64.8 N.A. 64.3
P-Site Identity: 100 0 60 100 N.A. 100 60 N.A. 80 6.6 60 80 N.A. N.A. 0 N.A. 46.6
P-Site Similarity: 100 0 93.3 100 N.A. 100 93.3 N.A. 93.3 46.6 93.3 93.3 N.A. N.A. 13.3 N.A. 53.3
Percent
Protein Identity: N.A. N.A. N.A. 23.1 N.A. 23.5
Protein Similarity: N.A. N.A. N.A. 39.8 N.A. 38.3
P-Site Identity: N.A. N.A. N.A. 6.6 N.A. 6.6
P-Site Similarity: N.A. N.A. N.A. 26.6 N.A. 26.6
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 8 0 0 0 8 0 8 0 0 22 15 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 8 0 0 29 15 8 0 8 65 0 0 0 0 0 0 % D
% Glu: 8 58 8 22 29 0 0 0 0 0 0 0 0 0 0 % E
% Phe: 8 0 0 0 0 0 0 8 0 0 0 0 8 0 8 % F
% Gly: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 8 0 8 0 8 36 8 0 0 22 0 0 0 0 0 % I
% Lys: 0 15 58 0 15 0 0 0 0 8 0 0 0 0 0 % K
% Leu: 0 0 8 8 0 29 65 15 0 0 15 0 15 65 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 8 0 8 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 % P
% Gln: 8 0 15 0 8 0 0 0 0 0 0 0 0 0 8 % Q
% Arg: 0 0 0 8 8 0 8 8 0 0 0 8 65 0 0 % R
% Ser: 0 0 0 15 8 8 0 43 15 15 36 65 8 8 8 % S
% Thr: 58 8 0 0 0 0 0 0 8 8 22 0 0 8 0 % T
% Val: 0 0 0 0 0 0 8 0 8 43 0 0 0 8 0 % V
% Trp: 0 0 0 8 0 0 0 0 0 0 0 8 0 8 65 % W
% Tyr: 0 0 0 8 0 0 0 0 0 0 0 0 0 0 8 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _