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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4B All Species: 8.48
Human Site: T213 Identified Species: 14.36
UniProt: Q9Y4P1 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0.15
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9Y4P1 NP_037457.3 393 44309 T213 F P A G A E V T N R P S P W R
Chimpanzee Pan troglodytes XP_001162556 306 34202 N130 A D S D R H C N G F P A G A E
Rhesus Macaque Macaca mulatta XP_001097251 398 45435 A215 N Q S K G T S A H C P A W K P
Dog Lupus familis XP_851977 394 44349 T214 F P A G A E V T N R L A P W R
Cat Felis silvestris
Mouse Mus musculus Q8BGE6 393 44357 T213 F P A G A E V T N R P S A W R
Rat Rattus norvegicus NP_001119770 406 45887 A216 S Q S K G T S A P C L A W K P
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513122 528 59425 N349 P S G A E V T N R P T Q W K P
Chicken Gallus gallus Q6PZ02 393 44512 V212 G Y P E E A G V R D K L S L W
Frog Xenopus laevis Q640G7 384 43139 D204 N G Y T G D S D P S C A Q W K
Zebra Danio Brachydanio rerio Q6DG88 394 44435 L213 D L E G A C A L A E E E T A L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_624577 382 43001 P200 G D A P L K A P S Q W K P L L
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001198708 393 44309 E216 R R T N G L M E G A C V S P G
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 G235 A S Y G L A A G S W V G P Y A
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 I302 P S A T A R C I Q A L A D E K
Conservation
Percent
Protein Identity: 100 74.8 52.5 94.1 N.A. 94.9 52.4 N.A. 65.1 86 73.7 72.8 N.A. N.A. 49.1 N.A. 49.8
Protein Similarity: 100 75.5 72.6 96.1 N.A. 96.6 70.9 N.A. 70 92.3 87 83.2 N.A. N.A. 64.8 N.A. 64.3
P-Site Identity: 100 6.6 6.6 86.6 N.A. 93.3 0 N.A. 0 0 6.6 13.3 N.A. N.A. 13.3 N.A. 0
P-Site Similarity: 100 20 26.6 93.3 N.A. 93.3 13.3 N.A. 0 0 26.6 13.3 N.A. N.A. 33.3 N.A. 6.6
Percent
Protein Identity: N.A. N.A. N.A. 23.1 N.A. 23.5
Protein Similarity: N.A. N.A. N.A. 39.8 N.A. 38.3
P-Site Identity: N.A. N.A. N.A. 13.3 N.A. 13.3
P-Site Similarity: N.A. N.A. N.A. 26.6 N.A. 26.6
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 15 0 36 8 36 15 22 15 8 15 0 43 8 15 8 % A
% Cys: 0 0 0 0 0 8 15 0 0 15 15 0 0 0 0 % C
% Asp: 8 15 0 8 0 8 0 8 0 8 0 0 8 0 0 % D
% Glu: 0 0 8 8 15 22 0 8 0 8 8 8 0 8 8 % E
% Phe: 22 0 0 0 0 0 0 0 0 8 0 0 0 0 0 % F
% Gly: 15 8 8 36 29 0 8 8 15 0 0 8 8 0 8 % G
% His: 0 0 0 0 0 8 0 0 8 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 8 0 0 0 0 0 0 0 % I
% Lys: 0 0 0 15 0 8 0 0 0 0 8 8 0 22 15 % K
% Leu: 0 8 0 0 15 8 0 8 0 0 22 8 0 15 15 % L
% Met: 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 % M
% Asn: 15 0 0 8 0 0 0 15 22 0 0 0 0 0 0 % N
% Pro: 15 22 8 8 0 0 0 8 15 8 29 0 29 8 22 % P
% Gln: 0 15 0 0 0 0 0 0 8 8 0 8 8 0 0 % Q
% Arg: 8 8 0 0 8 8 0 0 15 22 0 0 0 0 22 % R
% Ser: 8 22 22 0 0 0 22 0 15 8 0 15 15 0 0 % S
% Thr: 0 0 8 15 0 15 8 22 0 0 8 0 8 0 0 % T
% Val: 0 0 0 0 0 8 22 8 0 0 8 8 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 8 8 0 22 29 8 % W
% Tyr: 0 8 15 0 0 0 0 0 0 0 0 0 0 8 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _