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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4B All Species: 26.67
Human Site: Y123 Identified Species: 45.13
UniProt: Q9Y4P1 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9Y4P1 NP_037457.3 393 44309 Y123 I D R K D S Y Y S I H Q I A Q
Chimpanzee Pan troglodytes XP_001162556 306 34202 A40 S Y F S V L N A F I D R K D S
Rhesus Macaque Macaca mulatta XP_001097251 398 45435 C125 F L D R K D C C Y S I H Q M A
Dog Lupus familis XP_851977 394 44349 Y124 I D R K D S Y Y S I H Q I A Q
Cat Felis silvestris
Mouse Mus musculus Q8BGE6 393 44357 Y123 L D R K D S Y Y S I H Q I A Q
Rat Rattus norvegicus NP_001119770 406 45887 Y126 L D R K D C C Y S I H Q M A Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513122 528 59425 Y259 I D K K D S Y Y S I H Q I A Q
Chicken Gallus gallus Q6PZ02 393 44512 Y122 I D K K D S Y Y S I H Q I A Q
Frog Xenopus laevis Q640G7 384 43139 F114 Y L N I L T A F L D K K D S Y
Zebra Danio Brachydanio rerio Q6DG88 394 44435 Y123 I D K K D S Y Y S I H Q I A Q
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_624577 382 43001 L110 N S T Y L K I L E R F E D K R
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001198708 393 44309 D126 Q L F L D K K D S C F S I H Q
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 K145 L Q L D F S S K I L M T Y R K
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 F212 A M R I K T T F S D L T G F S
Conservation
Percent
Protein Identity: 100 74.8 52.5 94.1 N.A. 94.9 52.4 N.A. 65.1 86 73.7 72.8 N.A. N.A. 49.1 N.A. 49.8
Protein Similarity: 100 75.5 72.6 96.1 N.A. 96.6 70.9 N.A. 70 92.3 87 83.2 N.A. N.A. 64.8 N.A. 64.3
P-Site Identity: 100 6.6 0 100 N.A. 93.3 73.3 N.A. 93.3 93.3 0 93.3 N.A. N.A. 0 N.A. 26.6
P-Site Similarity: 100 13.3 6.6 100 N.A. 100 86.6 N.A. 100 100 26.6 100 N.A. N.A. 13.3 N.A. 26.6
Percent
Protein Identity: N.A. N.A. N.A. 23.1 N.A. 23.5
Protein Similarity: N.A. N.A. N.A. 39.8 N.A. 38.3
P-Site Identity: N.A. N.A. N.A. 6.6 N.A. 13.3
P-Site Similarity: N.A. N.A. N.A. 26.6 N.A. 26.6
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 0 0 0 0 0 8 8 0 0 0 0 0 50 8 % A
% Cys: 0 0 0 0 0 8 15 8 0 8 0 0 0 0 0 % C
% Asp: 0 50 8 8 58 8 0 8 0 15 8 0 15 8 0 % D
% Glu: 0 0 0 0 0 0 0 0 8 0 0 8 0 0 0 % E
% Phe: 8 0 15 0 8 0 0 15 8 0 15 0 0 8 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 50 8 0 8 0 % H
% Ile: 36 0 0 15 0 0 8 0 8 58 8 0 50 0 0 % I
% Lys: 0 0 22 50 15 15 8 8 0 0 8 8 8 8 8 % K
% Leu: 22 22 8 8 15 8 0 8 8 8 8 0 0 0 0 % L
% Met: 0 8 0 0 0 0 0 0 0 0 8 0 8 8 0 % M
% Asn: 8 0 8 0 0 0 8 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 8 8 0 0 0 0 0 0 0 0 0 50 8 0 58 % Q
% Arg: 0 0 36 8 0 0 0 0 0 8 0 8 0 8 8 % R
% Ser: 8 8 0 8 0 50 8 0 65 8 0 8 0 8 15 % S
% Thr: 0 0 8 0 0 15 8 0 0 0 0 15 0 0 0 % T
% Val: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 8 8 0 8 0 0 43 50 8 0 0 0 8 0 8 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _