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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4B All Species: 36.67
Human Site: Y265 Identified Species: 62.05
UniProt: Q9Y4P1 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9Y4P1 NP_037457.3 393 44309 Y265 G K P N S A H Y F I G Y V G E
Chimpanzee Pan troglodytes XP_001162556 306 34202 V181 M M P Q S L G V I G G K P N S
Rhesus Macaque Macaca mulatta XP_001097251 398 45435 Y266 G K P N N A Y Y F I G F L G D
Dog Lupus familis XP_851977 394 44349 Y266 G K P N S A H Y F I G Y V G E
Cat Felis silvestris
Mouse Mus musculus Q8BGE6 393 44357 Y265 G K P N S A H Y F I G Y V G E
Rat Rattus norvegicus NP_001119770 406 45887 Y267 G K P N N A Y Y F I G S L G D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513122 528 59425 Y400 G K P N S A H Y F I G Y V G G
Chicken Gallus gallus Q6PZ02 393 44512 Y265 G K P N S A H Y F I G Y V G E
Frog Xenopus laevis Q640G7 384 43139 Y256 G R P N S A H Y F I G Y V G D
Zebra Danio Brachydanio rerio Q6DG88 394 44435 Y267 G K P N S A H Y F I G F V G D
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_624577 382 43001 G252 T H A L Y F I G C V G N E V I
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001198708 393 44309 P268 L G V I G G K P N H A H Y F I
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 C287 R G G A P I L C I E D A T K S
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 Y360 I D K I N Q V Y E E A L I S T
Conservation
Percent
Protein Identity: 100 74.8 52.5 94.1 N.A. 94.9 52.4 N.A. 65.1 86 73.7 72.8 N.A. N.A. 49.1 N.A. 49.8
Protein Similarity: 100 75.5 72.6 96.1 N.A. 96.6 70.9 N.A. 70 92.3 87 83.2 N.A. N.A. 64.8 N.A. 64.3
P-Site Identity: 100 20 66.6 100 N.A. 100 66.6 N.A. 93.3 100 86.6 86.6 N.A. N.A. 6.6 N.A. 0
P-Site Similarity: 100 20 100 100 N.A. 100 93.3 N.A. 93.3 100 100 100 N.A. N.A. 13.3 N.A. 6.6
Percent
Protein Identity: N.A. N.A. N.A. 23.1 N.A. 23.5
Protein Similarity: N.A. N.A. N.A. 39.8 N.A. 38.3
P-Site Identity: N.A. N.A. N.A. 0 N.A. 6.6
P-Site Similarity: N.A. N.A. N.A. 0 N.A. 20
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 8 8 0 65 0 0 0 0 15 8 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 8 8 0 0 0 0 0 0 % C
% Asp: 0 8 0 0 0 0 0 0 0 0 8 0 0 0 29 % D
% Glu: 0 0 0 0 0 0 0 0 8 15 0 0 8 0 29 % E
% Phe: 0 0 0 0 0 8 0 0 65 0 0 15 0 8 0 % F
% Gly: 65 15 8 0 8 8 8 8 0 8 79 0 0 65 8 % G
% His: 0 8 0 0 0 0 50 0 0 8 0 8 0 0 0 % H
% Ile: 8 0 0 15 0 8 8 0 15 65 0 0 8 0 15 % I
% Lys: 0 58 8 0 0 0 8 0 0 0 0 8 0 8 0 % K
% Leu: 8 0 0 8 0 8 8 0 0 0 0 8 15 0 0 % L
% Met: 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 65 22 0 0 0 8 0 0 8 0 8 0 % N
% Pro: 0 0 72 0 8 0 0 8 0 0 0 0 8 0 0 % P
% Gln: 0 0 0 8 0 8 0 0 0 0 0 0 0 0 0 % Q
% Arg: 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 0 0 0 0 58 0 0 0 0 0 0 8 0 8 15 % S
% Thr: 8 0 0 0 0 0 0 0 0 0 0 0 8 0 8 % T
% Val: 0 0 8 0 0 0 8 8 0 8 0 0 50 8 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 8 0 15 72 0 0 0 43 8 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _