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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4B All Species: 26.97
Human Site: Y276 Identified Species: 45.64
UniProt: Q9Y4P1 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9Y4P1 NP_037457.3 393 44309 Y276 Y V G E E L I Y L D P H T T Q
Chimpanzee Pan troglodytes XP_001162556 306 34202 F192 K P N S A H Y F I G Y V G E E
Rhesus Macaque Macaca mulatta XP_001097251 398 45435 F277 F L G D E L I F L D P H T T Q
Dog Lupus familis XP_851977 394 44349 Y277 Y V G E E L I Y L D P H T T Q
Cat Felis silvestris
Mouse Mus musculus Q8BGE6 393 44357 Y276 Y V G E E L I Y L D P H T T Q
Rat Rattus norvegicus NP_001119770 406 45887 F278 S L G D E L I F L D P H T T Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513122 528 59425 Y411 Y V G G E L I Y L D P H T T Q
Chicken Gallus gallus Q6PZ02 393 44512 Y276 Y V G E E L I Y L D P H T T Q
Frog Xenopus laevis Q640G7 384 43139 Y267 Y V G D E L I Y L D P H T T Q
Zebra Danio Brachydanio rerio Q6DG88 394 44435 Y278 F V G D E L I Y L D P H T T Q
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_624577 382 43001 P263 N E V I Y L D P H T T Q K S G
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001198708 393 44309 G279 H Y F I G V L G D E M V Y L D
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 F298 A T K S C L E F S K G Q S E W
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 P371 L I S T L Q L P Q S I G I A G
Conservation
Percent
Protein Identity: 100 74.8 52.5 94.1 N.A. 94.9 52.4 N.A. 65.1 86 73.7 72.8 N.A. N.A. 49.1 N.A. 49.8
Protein Similarity: 100 75.5 72.6 96.1 N.A. 96.6 70.9 N.A. 70 92.3 87 83.2 N.A. N.A. 64.8 N.A. 64.3
P-Site Identity: 100 0 73.3 100 N.A. 100 73.3 N.A. 93.3 100 93.3 86.6 N.A. N.A. 6.6 N.A. 0
P-Site Similarity: 100 20 100 100 N.A. 100 93.3 N.A. 93.3 100 100 100 N.A. N.A. 13.3 N.A. 26.6
Percent
Protein Identity: N.A. N.A. N.A. 23.1 N.A. 23.5
Protein Similarity: N.A. N.A. N.A. 39.8 N.A. 38.3
P-Site Identity: N.A. N.A. N.A. 6.6 N.A. 0
P-Site Similarity: N.A. N.A. N.A. 20 N.A. 13.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 0 0 0 8 0 0 0 0 0 0 0 0 8 0 % A
% Cys: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 29 0 0 8 0 8 65 0 0 0 0 8 % D
% Glu: 0 8 0 29 65 0 8 0 0 8 0 0 0 15 8 % E
% Phe: 15 0 8 0 0 0 0 29 0 0 0 0 0 0 0 % F
% Gly: 0 0 65 8 8 0 0 8 0 8 8 8 8 0 15 % G
% His: 8 0 0 0 0 8 0 0 8 0 0 65 0 0 0 % H
% Ile: 0 8 0 15 0 0 65 0 8 0 8 0 8 0 0 % I
% Lys: 8 0 8 0 0 0 0 0 0 8 0 0 8 0 0 % K
% Leu: 8 15 0 0 8 79 15 0 65 0 0 0 0 8 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 8 0 0 0 0 % M
% Asn: 8 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 8 0 0 0 0 0 15 0 0 65 0 0 0 0 % P
% Gln: 0 0 0 0 0 8 0 0 8 0 0 15 0 0 65 % Q
% Arg: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 8 0 8 15 0 0 0 0 8 8 0 0 8 8 0 % S
% Thr: 0 8 0 8 0 0 0 0 0 8 8 0 65 65 0 % T
% Val: 0 50 8 0 0 8 0 0 0 0 0 15 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 % W
% Tyr: 43 8 0 0 8 0 8 50 0 0 8 0 8 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _