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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4B All Species: 24.85
Human Site: Y33 Identified Species: 42.05
UniProt: Q9Y4P1 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9Y4P1 NP_037457.3 393 44309 Y33 V W I L G R K Y S I F T E K D
Chimpanzee Pan troglodytes XP_001162556 306 34202
Rhesus Macaque Macaca mulatta XP_001097251 398 45435 H36 V W I L G K Q H L L K T E K S
Dog Lupus familis XP_851977 394 44349 Y34 V W I L G R K Y S I F T E K D
Cat Felis silvestris
Mouse Mus musculus Q8BGE6 393 44357 Y33 V W I L G R K Y S I F T E K D
Rat Rattus norvegicus NP_001119770 406 45887 H36 V W I L G K P H L L K T E K S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513122 528 59425 Y169 V W I L G R K Y T I F T E K E
Chicken Gallus gallus Q6PZ02 393 44512 S33 W I L G R K Y S V F T E K E E
Frog Xenopus laevis Q640G7 384 43139 Y33 V W V L G R K Y S A L T E K E
Zebra Danio Brachydanio rerio Q6DG88 394 44435 F33 V W I L G K Q F S A L T E K D
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_624577 382 43001 I33 L G K K Y N A I R E L D A I R
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001198708 393 44309 Y40 I W I L G K K Y D L S Q H Q L
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 N36 S D S G P S D N K S K F T L W
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 Y72 V W C L G C S Y T L D I K Q Y
Conservation
Percent
Protein Identity: 100 74.8 52.5 94.1 N.A. 94.9 52.4 N.A. 65.1 86 73.7 72.8 N.A. N.A. 49.1 N.A. 49.8
Protein Similarity: 100 75.5 72.6 96.1 N.A. 96.6 70.9 N.A. 70 92.3 87 83.2 N.A. N.A. 64.8 N.A. 64.3
P-Site Identity: 100 0 53.3 100 N.A. 100 53.3 N.A. 86.6 0 73.3 66.6 N.A. N.A. 0 N.A. 40
P-Site Similarity: 100 0 80 100 N.A. 100 73.3 N.A. 100 33.3 86.6 86.6 N.A. N.A. 6.6 N.A. 66.6
Percent
Protein Identity: N.A. N.A. N.A. 23.1 N.A. 23.5
Protein Similarity: N.A. N.A. N.A. 39.8 N.A. 38.3
P-Site Identity: N.A. N.A. N.A. 0 N.A. 33.3
P-Site Similarity: N.A. N.A. N.A. 0 N.A. 60
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 8 0 0 15 0 0 8 0 0 % A
% Cys: 0 0 8 0 0 8 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 8 0 0 0 0 8 0 8 0 8 8 0 0 29 % D
% Glu: 0 0 0 0 0 0 0 0 0 8 0 8 58 8 22 % E
% Phe: 0 0 0 0 0 0 0 8 0 8 29 8 0 0 0 % F
% Gly: 0 8 0 15 72 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 15 0 0 0 0 8 0 0 % H
% Ile: 8 8 58 0 0 0 0 8 0 29 0 8 0 8 0 % I
% Lys: 0 0 8 8 0 36 43 0 8 0 22 0 15 58 0 % K
% Leu: 8 0 8 72 0 0 0 0 15 29 22 0 0 8 8 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 8 0 8 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 8 0 8 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 15 0 0 0 0 8 0 15 0 % Q
% Arg: 0 0 0 0 8 36 0 0 8 0 0 0 0 0 8 % R
% Ser: 8 0 8 0 0 8 8 8 36 8 8 0 0 0 15 % S
% Thr: 0 0 0 0 0 0 0 0 15 0 8 58 8 0 0 % T
% Val: 65 0 8 0 0 0 0 0 8 0 0 0 0 0 0 % V
% Trp: 8 72 0 0 0 0 0 0 0 0 0 0 0 0 8 % W
% Tyr: 0 0 0 0 8 0 8 50 0 0 0 0 0 0 8 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _