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Updated November 2019
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Protein Info
Short Name:
IRS4
Full Name:
Insulin receptor substrate 4
Alias:
IRS-4; PY160
Type:
Adapter/scaffold protein
Mass (Da):
133768
Number AA:
1257
UniProt ID:
O14654
International Prot ID:
IPI00020729
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005886
Uniprot
OncoNet
Molecular Function:
GO:0005070
GO:0005158
PhosphoSite+
KinaseNET
Biological Process:
GO:0007165
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
T7
_
M
A
S
C
S
F
T
R
D
Q
A
T
R
R
Site 2
T12
S
F
T
R
D
Q
A
T
R
R
L
R
G
A
A
Site 3
S57
C
P
G
A
M
W
L
S
T
A
T
G
S
R
S
Site 4
T58
P
G
A
M
W
L
S
T
A
T
G
S
R
S
D
Site 5
T60
A
M
W
L
S
T
A
T
G
S
R
S
D
S
E
Site 6
S62
W
L
S
T
A
T
G
S
R
S
D
S
E
S
E
Site 7
S64
S
T
A
T
G
S
R
S
D
S
E
S
E
E
E
Site 8
S66
A
T
G
S
R
S
D
S
E
S
E
E
E
D
L
Site 9
S68
G
S
R
S
D
S
E
S
E
E
E
D
L
P
V
Site 10
Y84
E
E
V
C
K
R
G
Y
L
R
K
Q
K
H
G
Site 11
Y95
Q
K
H
G
H
R
R
Y
F
V
L
K
L
E
T
Site 12
Y111
D
A
P
A
R
L
E
Y
Y
E
N
A
R
K
F
Site 13
Y112
A
P
A
R
L
E
Y
Y
E
N
A
R
K
F
R
Site 14
S121
N
A
R
K
F
R
H
S
V
R
A
A
A
A
A
Site 15
Y151
P
R
R
V
I
T
L
Y
Q
C
F
S
V
S
Q
Site 16
S157
L
Y
Q
C
F
S
V
S
Q
R
A
D
A
R
Y
Site 17
Y164
S
Q
R
A
D
A
R
Y
R
H
L
I
A
L
F
Site 18
S189
E
N
E
S
E
Q
E
S
W
Y
L
L
L
S
R
Site 19
Y191
E
S
E
Q
E
S
W
Y
L
L
L
S
R
L
I
Site 20
S201
L
S
R
L
I
L
E
S
K
R
R
R
C
G
T
Site 21
T208
S
K
R
R
R
C
G
T
L
G
A
Q
P
D
G
Site 22
Y291
R
C
G
H
S
E
Q
Y
F
F
L
E
V
G
R
Site 23
Y336
R
A
L
C
A
D
E
Y
R
A
R
C
R
S
Y
Site 24
S342
E
Y
R
A
R
C
R
S
Y
S
I
S
I
G
A
Site 25
Y343
Y
R
A
R
C
R
S
Y
S
I
S
I
G
A
H
Site 26
S344
R
A
R
C
R
S
Y
S
I
S
I
G
A
H
L
Site 27
T396
G
E
D
E
M
L
F
T
R
R
F
V
T
P
S
Site 28
T401
L
F
T
R
R
F
V
T
P
S
E
P
V
A
H
Site 29
S403
T
R
R
F
V
T
P
S
E
P
V
A
H
S
R
Site 30
S409
P
S
E
P
V
A
H
S
R
R
G
R
L
H
L
Site 31
S422
H
L
P
R
G
R
R
S
R
R
A
V
S
V
P
Site 32
S427
R
R
S
R
R
A
V
S
V
P
A
S
F
F
R
Site 33
S439
F
F
R
R
L
A
P
S
P
A
R
P
R
H
P
Site 34
S457
P
N
N
G
A
R
L
S
S
E
V
S
G
S
G
Site 35
S458
N
N
G
A
R
L
S
S
E
V
S
G
S
G
S
Site 36
S461
A
R
L
S
S
E
V
S
G
S
G
S
G
N
F
Site 37
S463
L
S
S
E
V
S
G
S
G
S
G
N
F
G
E
Site 38
S465
S
E
V
S
G
S
G
S
G
N
F
G
E
E
G
Site 39
S483
G
K
E
D
Q
E
G
S
G
G
D
Y
M
P
M
Site 40
Y487
Q
E
G
S
G
G
D
Y
M
P
M
N
N
W
G
Site 41
S501
G
S
G
N
G
R
G
S
G
G
G
Q
G
S
N
Site 42
S507
G
S
G
G
G
Q
G
S
N
G
Q
G
S
S
S
Site 43
S512
Q
G
S
N
G
Q
G
S
S
S
H
S
S
G
G
Site 44
S513
G
S
N
G
Q
G
S
S
S
H
S
S
G
G
N
Site 45
S514
S
N
G
Q
G
S
S
S
H
S
S
G
G
N
Q
Site 46
S516
G
Q
G
S
S
S
H
S
S
G
G
N
Q
C
S
Site 47
S517
Q
G
S
S
S
H
S
S
G
G
N
Q
C
S
G
Site 48
S523
S
S
G
G
N
Q
C
S
G
E
G
Q
G
S
R
Site 49
S529
C
S
G
E
G
Q
G
S
R
G
G
Q
G
S
N
Site 50
S535
G
S
R
G
G
Q
G
S
N
G
Q
G
S
G
G
Site 51
S540
Q
G
S
N
G
Q
G
S
G
G
N
Q
C
S
R
Site 52
S546
G
S
G
G
N
Q
C
S
R
D
G
Q
G
T
A
Site 53
T552
C
S
R
D
G
Q
G
T
A
G
G
H
G
S
G
Site 54
S558
G
T
A
G
G
H
G
S
G
G
G
Q
R
P
G
Site 55
S570
R
P
G
G
G
H
G
S
G
G
G
Q
G
P
G
Site 56
S582
G
P
G
D
G
H
G
S
G
G
G
K
N
S
G
Site 57
S588
G
S
G
G
G
K
N
S
G
G
G
K
G
S
G
Site 58
S594
N
S
G
G
G
K
G
S
G
S
G
K
G
S
D
Site 59
S596
G
G
G
K
G
S
G
S
G
K
G
S
D
G
D
Site 60
S600
G
S
G
S
G
K
G
S
D
G
D
G
E
R
G
Site 61
S609
G
D
G
E
R
G
K
S
L
K
K
R
S
Y
F
Site 62
S614
G
K
S
L
K
K
R
S
Y
F
G
K
L
T
Q
Site 63
Y615
K
S
L
K
K
R
S
Y
F
G
K
L
T
Q
S
Site 64
T620
R
S
Y
F
G
K
L
T
Q
S
K
Q
Q
Q
M
Site 65
S622
Y
F
G
K
L
T
Q
S
K
Q
Q
Q
M
P
P
Site 66
S649
G
T
G
G
K
G
K
S
G
G
R
F
R
L
Y
Site 67
Y656
S
G
G
R
F
R
L
Y
F
C
V
D
R
G
A
Site 68
T664
F
C
V
D
R
G
A
T
K
E
C
K
E
A
K
Site 69
Y700
D
E
D
E
D
D
P
Y
V
P
M
R
P
G
V
Site 70
T709
P
M
R
P
G
V
A
T
P
L
V
S
S
S
D
Site 71
S714
V
A
T
P
L
V
S
S
S
D
Y
M
P
M
A
Site 72
S715
A
T
P
L
V
S
S
S
D
Y
M
P
M
A
P
Site 73
Y717
P
L
V
S
S
S
D
Y
M
P
M
A
P
Q
N
Site 74
S726
P
M
A
P
Q
N
V
S
A
S
K
K
R
H
S
Site 75
S728
A
P
Q
N
V
S
A
S
K
K
R
H
S
R
S
Site 76
S733
S
A
S
K
K
R
H
S
R
S
P
F
E
D
S
Site 77
S735
S
K
K
R
H
S
R
S
P
F
E
D
S
R
G
Site 78
Y743
P
F
E
D
S
R
G
Y
M
M
M
F
P
R
V
Site 79
S751
M
M
M
F
P
R
V
S
P
P
P
A
P
S
P
Site 80
S757
V
S
P
P
P
A
P
S
P
P
K
A
P
D
T
Site 81
T764
S
P
P
K
A
P
D
T
N
K
E
D
D
S
K
Site 82
S770
D
T
N
K
E
D
D
S
K
D
N
D
S
E
S
Site 83
S775
D
D
S
K
D
N
D
S
E
S
D
Y
M
F
M
Site 84
S777
S
K
D
N
D
S
E
S
D
Y
M
F
M
A
P
Site 85
Y779
D
N
D
S
E
S
D
Y
M
F
M
A
P
G
A
Site 86
S800
P
R
N
P
Q
G
G
S
S
S
K
S
W
S
S
Site 87
S801
R
N
P
Q
G
G
S
S
S
K
S
W
S
S
Y
Site 88
S802
N
P
Q
G
G
S
S
S
K
S
W
S
S
Y
F
Site 89
S804
Q
G
G
S
S
S
K
S
W
S
S
Y
F
S
L
Site 90
S806
G
S
S
S
K
S
W
S
S
Y
F
S
L
P
N
Site 91
S807
S
S
S
K
S
W
S
S
Y
F
S
L
P
N
P
Site 92
Y808
S
S
K
S
W
S
S
Y
F
S
L
P
N
P
F
Site 93
S810
K
S
W
S
S
Y
F
S
L
P
N
P
F
R
S
Site 94
S817
S
L
P
N
P
F
R
S
S
P
L
G
Q
N
D
Site 95
S818
L
P
N
P
F
R
S
S
P
L
G
Q
N
D
N
Site 96
S826
P
L
G
Q
N
D
N
S
E
Y
V
P
M
L
P
Site 97
Y828
G
Q
N
D
N
S
E
Y
V
P
M
L
P
G
K
Site 98
S846
R
G
L
D
K
E
V
S
Y
N
W
D
P
K
D
Site 99
S856
W
D
P
K
D
A
A
S
K
P
S
G
E
G
S
Site 100
S859
K
D
A
A
S
K
P
S
G
E
G
S
F
S
K
Site 101
S863
S
K
P
S
G
E
G
S
F
S
K
P
G
D
G
Site 102
S865
P
S
G
E
G
S
F
S
K
P
G
D
G
G
S
Site 103
S872
S
K
P
G
D
G
G
S
P
S
K
P
S
D
H
Site 104
S874
P
G
D
G
G
S
P
S
K
P
S
D
H
E
P
Site 105
S877
G
G
S
P
S
K
P
S
D
H
E
P
P
K
N
Site 106
S893
A
K
R
P
N
R
L
S
F
I
T
K
G
Y
K
Site 107
T896
P
N
R
L
S
F
I
T
K
G
Y
K
I
K
P
Site 108
S917
H
E
Q
R
E
A
D
S
S
S
D
Y
V
N
M
Site 109
S918
E
Q
R
E
A
D
S
S
S
D
Y
V
N
M
D
Site 110
S919
Q
R
E
A
D
S
S
S
D
Y
V
N
M
D
F
Site 111
Y921
E
A
D
S
S
S
D
Y
V
N
M
D
F
T
K
Site 112
T927
D
Y
V
N
M
D
F
T
K
R
E
S
N
T
P
Site 113
S931
M
D
F
T
K
R
E
S
N
T
P
A
P
S
T
Site 114
T933
F
T
K
R
E
S
N
T
P
A
P
S
T
Q
G
Site 115
S937
E
S
N
T
P
A
P
S
T
Q
G
L
P
D
S
Site 116
T938
S
N
T
P
A
P
S
T
Q
G
L
P
D
S
W
Site 117
S944
S
T
Q
G
L
P
D
S
W
G
I
I
A
E
P
Site 118
S954
I
I
A
E
P
R
Q
S
A
F
S
N
Y
V
N
Site 119
S957
E
P
R
Q
S
A
F
S
N
Y
V
N
V
E
F
Site 120
Y959
R
Q
S
A
F
S
N
Y
V
N
V
E
F
G
V
Site 121
S976
P
N
P
A
N
D
L
S
D
L
L
R
A
I
P
Site 122
S989
I
P
R
A
N
P
L
S
L
D
S
A
R
W
P
Site 123
S992
A
N
P
L
S
L
D
S
A
R
W
P
L
P
P
Site 124
S1007
L
P
L
S
A
T
G
S
N
A
I
E
E
E
G
Site 125
Y1016
A
I
E
E
E
G
D
Y
I
E
V
I
F
N
S
Site 126
Y1038
L
A
D
S
A
I
R
Y
D
A
E
T
G
R
I
Site 127
T1042
A
I
R
Y
D
A
E
T
G
R
I
Y
V
V
D
Site 128
Y1046
D
A
E
T
G
R
I
Y
V
V
D
P
F
S
E
Site 129
S1059
S
E
C
C
M
D
I
S
L
S
P
S
R
C
S
Site 130
S1061
C
C
M
D
I
S
L
S
P
S
R
C
S
E
P
Site 131
S1063
M
D
I
S
L
S
P
S
R
C
S
E
P
P
P
Site 132
S1066
S
L
S
P
S
R
C
S
E
P
P
P
V
A
R
Site 133
S1087
Q
E
R
R
R
P
Q
S
R
S
Q
S
F
F
A
Site 134
S1089
R
R
R
P
Q
S
R
S
Q
S
F
F
A
A
A
Site 135
S1091
R
P
Q
S
R
S
Q
S
F
F
A
A
A
R
A
Site 136
T1105
A
A
V
S
A
F
P
T
D
S
L
E
R
D
L
Site 137
S1107
V
S
A
F
P
T
D
S
L
E
R
D
L
S
P
Site 138
S1113
D
S
L
E
R
D
L
S
P
S
S
A
P
A
V
Site 139
S1115
L
E
R
D
L
S
P
S
S
A
P
A
V
A
S
Site 140
S1116
E
R
D
L
S
P
S
S
A
P
A
V
A
S
A
Site 141
S1157
A
A
A
A
G
F
D
S
A
S
A
R
W
F
Q
Site 142
S1159
A
A
G
F
D
S
A
S
A
R
W
F
Q
P
V
Site 143
S1185
A
Q
D
V
A
G
G
S
N
P
G
A
H
N
P
Site 144
S1219
A
P
E
P
P
P
R
S
R
R
V
P
R
P
P
Site 145
S1231
R
P
P
E
R
E
D
S
D
N
D
D
D
T
H
Site 146
T1237
D
S
D
N
D
D
D
T
H
V
R
M
D
F
A
Site 147
S1252
R
R
D
N
Q
F
D
S
P
K
R
G
R
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation