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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
NUAK1
Full Name:
NUAK family SNF1-like kinase 1
Alias:
AMP-activated protein kinase family member 5; ARK5; EC 2.7.11.1; KIAA053; KIAA0537; Kinase NuaK1; NUAK family, SNF1-like kinase, 1; NUAK2; Probable KIAA0537
Type:
EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMKL family; NuaK subfamily
Mass (Da):
74305
Number AA:
661
UniProt ID:
O60285
International Prot ID:
IPI00028566
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0000287
GO:0005515
PhosphoSite+
KinaseNET
Biological Process:
GO:0006468
Phosida
TranscriptoNet
STRING
Kinexus Products
NUAK family SNF1-like kinase 1; AMPK-related protein kinase 5 T211 phosphosite-specific antibody AB-PK737#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK737#Cdc25C (205-225), KinSub - Cdc25C phosphatase (V205-R225, human) peptide; Chktide peptide - Powder PE-01ACN95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ACN95#NUAK family SNF1-like kinase 1 / AMPK-related protein kinase 5 (F208-G214, human) pT211 phosphopeptide - Powder PE-04AKS99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AKS99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S22
L
G
L
G
A
P
G
S
P
R
E
A
V
A
G
Site 2
T60
H
R
Y
E
L
Q
E
T
L
G
K
G
T
Y
G
Site 3
T73
Y
G
K
V
K
R
A
T
E
R
F
S
G
R
V
Site 4
S77
K
R
A
T
E
R
F
S
G
R
V
V
A
I
K
Site 5
S85
G
R
V
V
A
I
K
S
I
R
K
D
K
I
K
Site 6
S109
R
E
I
E
I
M
S
S
L
N
H
P
H
I
I
Site 7
Y119
H
P
H
I
I
S
I
Y
E
V
F
E
N
K
D
Site 8
Y134
K
I
V
I
I
M
E
Y
A
S
K
G
E
L
Y
Site 9
Y141
Y
A
S
K
G
E
L
Y
D
Y
I
S
E
R
R
Site 10
Y143
S
K
G
E
L
Y
D
Y
I
S
E
R
R
R
L
Site 11
S145
G
E
L
Y
D
Y
I
S
E
R
R
R
L
S
E
Site 12
S151
I
S
E
R
R
R
L
S
E
R
E
T
R
H
F
Site 13
T155
R
R
L
S
E
R
E
T
R
H
F
F
R
Q
I
Site 14
Y203
D
F
G
L
S
N
L
Y
Q
K
D
K
F
L
Q
Site 15
T211
Q
K
D
K
F
L
Q
T
F
C
G
S
P
L
Y
NUAK family SNF1-like kinase 1; AMPK-related protein kinase 5 T211 phosphosite-specific antibody AB-PK737#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK737#NUAK family SNF1-like kinase 1 / AMPK-related protein kinase 5 (F208-G214, human) pT211 phosphopeptide - Powder PE-04AKS99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AKS99
Site 16
S220
C
G
S
P
L
Y
A
S
P
E
I
V
N
G
R
Site 17
Y229
E
I
V
N
G
R
P
Y
R
G
P
E
V
D
S
Site 18
S266
K
N
L
I
R
Q
I
S
S
G
E
Y
R
E
P
Site 19
Y270
R
Q
I
S
S
G
E
Y
R
E
P
T
Q
P
S
Site 20
T274
S
G
E
Y
R
E
P
T
Q
P
S
D
A
R
G
Site 21
T296
V
N
P
D
R
R
A
T
I
E
D
I
A
N
H
Site 22
S323
D
C
D
A
L
H
D
S
E
S
P
L
L
A
R
Site 23
T339
I
D
W
H
H
R
S
T
G
L
Q
A
D
T
E
Site 24
T345
S
T
G
L
Q
A
D
T
E
A
K
M
K
G
L
Site 25
T356
M
K
G
L
A
K
P
T
T
S
E
V
M
L
E
Site 26
T357
K
G
L
A
K
P
T
T
S
E
V
M
L
E
R
Site 27
S358
G
L
A
K
P
T
T
S
E
V
M
L
E
R
Q
Site 28
S367
V
M
L
E
R
Q
R
S
L
K
K
S
K
K
E
Site 29
S371
R
Q
R
S
L
K
K
S
K
K
E
N
D
F
A
Site 30
S388
G
Q
D
A
V
P
E
S
P
S
K
L
S
S
K
Site 31
S390
D
A
V
P
E
S
P
S
K
L
S
S
K
R
P
Site 32
S393
P
E
S
P
S
K
L
S
S
K
R
P
K
G
I
Site 33
S394
E
S
P
S
K
L
S
S
K
R
P
K
G
I
L
Site 34
S405
K
G
I
L
K
K
R
S
N
S
E
H
R
S
H
Site 35
S407
I
L
K
K
R
S
N
S
E
H
R
S
H
S
T
Site 36
S411
R
S
N
S
E
H
R
S
H
S
T
G
F
I
E
Site 37
S413
N
S
E
H
R
S
H
S
T
G
F
I
E
G
V
Site 38
T428
V
G
P
A
L
P
S
T
F
K
M
E
Q
D
L
Site 39
S444
R
T
G
V
L
L
P
S
S
P
E
A
E
V
P
Site 40
S445
T
G
V
L
L
P
S
S
P
E
A
E
V
P
G
Site 41
S455
A
E
V
P
G
K
L
S
P
K
Q
S
A
T
M
Site 42
S459
G
K
L
S
P
K
Q
S
A
T
M
P
K
K
G
Site 43
T461
L
S
P
K
Q
S
A
T
M
P
K
K
G
I
L
Site 44
T471
K
K
G
I
L
K
K
T
Q
Q
R
E
S
G
Y
Site 45
S476
K
K
T
Q
Q
R
E
S
G
Y
Y
S
S
P
E
Site 46
Y479
Q
Q
R
E
S
G
Y
Y
S
S
P
E
R
S
E
Site 47
S480
Q
R
E
S
G
Y
Y
S
S
P
E
R
S
E
S
Site 48
S481
R
E
S
G
Y
Y
S
S
P
E
R
S
E
S
S
Site 49
S485
Y
Y
S
S
P
E
R
S
E
S
S
E
L
L
D
Site 50
S487
S
S
P
E
R
S
E
S
S
E
L
L
D
S
N
Site 51
S488
S
P
E
R
S
E
S
S
E
L
L
D
S
N
D
Site 52
S493
E
S
S
E
L
L
D
S
N
D
V
M
G
S
S
Site 53
S499
D
S
N
D
V
M
G
S
S
I
P
S
P
S
P
Site 54
S500
S
N
D
V
M
G
S
S
I
P
S
P
S
P
P
Site 55
S503
V
M
G
S
S
I
P
S
P
S
P
P
D
P
A
Site 56
S505
G
S
S
I
P
S
P
S
P
P
D
P
A
R
V
Site 57
T513
P
P
D
P
A
R
V
T
S
H
S
L
S
C
R
Site 58
S514
P
D
P
A
R
V
T
S
H
S
L
S
C
R
R
Site 59
S516
P
A
R
V
T
S
H
S
L
S
C
R
R
K
G
Site 60
S518
R
V
T
S
H
S
L
S
C
R
R
K
G
I
L
Site 61
S528
R
K
G
I
L
K
H
S
S
K
Y
S
A
G
T
Site 62
S529
K
G
I
L
K
H
S
S
K
Y
S
A
G
T
M
Site 63
S532
L
K
H
S
S
K
Y
S
A
G
T
M
D
P
A
Site 64
S542
T
M
D
P
A
L
V
S
P
E
M
P
T
L
E
Site 65
T547
L
V
S
P
E
M
P
T
L
E
S
L
S
E
P
Site 66
S550
P
E
M
P
T
L
E
S
L
S
E
P
G
V
P
Site 67
S552
M
P
T
L
E
S
L
S
E
P
G
V
P
A
E
Site 68
S562
G
V
P
A
E
G
L
S
R
S
Y
S
R
P
S
Site 69
S564
P
A
E
G
L
S
R
S
Y
S
R
P
S
S
V
Site 70
Y565
A
E
G
L
S
R
S
Y
S
R
P
S
S
V
I
Site 71
S566
E
G
L
S
R
S
Y
S
R
P
S
S
V
I
S
Site 72
S569
S
R
S
Y
S
R
P
S
S
V
I
S
D
D
S
Site 73
S570
R
S
Y
S
R
P
S
S
V
I
S
D
D
S
V
Site 74
S573
S
R
P
S
S
V
I
S
D
D
S
V
L
S
S
Site 75
S576
S
S
V
I
S
D
D
S
V
L
S
S
D
S
F
Site 76
S580
S
D
D
S
V
L
S
S
D
S
F
D
L
L
D
Site 77
S582
D
S
V
L
S
S
D
S
F
D
L
L
D
L
Q
Site 78
S600
P
A
R
Q
R
I
R
S
C
V
S
A
E
N
F
Site 79
S603
Q
R
I
R
S
C
V
S
A
E
N
F
L
Q
I
Site 80
Y624
Q
N
R
P
R
P
Q
Y
L
K
R
Y
R
N
R
Site 81
S635
Y
R
N
R
L
A
D
S
S
F
S
L
L
T
D
Site 82
S636
R
N
R
L
A
D
S
S
F
S
L
L
T
D
M
Site 83
T647
L
T
D
M
D
D
V
T
Q
V
Y
K
Q
A
L
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation