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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
MBD4
Full Name:
Methyl-CpG-binding domain protein 4
Alias:
MED1; methyl-CpG binding domain 4; methyl-CpG binding domain protein 4; methyl-CpG-binding domain 4; methyl-CpG-binding endonuclease 1; methyl-CpG-binding protein MBD4; mismatch-specific DNA N-glycosylase
Type:
Deoxyribonuclease; EC 3.2.2.-; DNA binding protein; DNA repair; Hydrolase
Mass (Da):
66050
Number AA:
580
UniProt ID:
O95243
International Prot ID:
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005634
Uniprot
OncoNet
Molecular Function:
GO:0004520
GO:0005515
GO:0003696
PhosphoSite+
KinaseNET
Biological Process:
GO:0045008
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S8
M
G
T
T
G
L
E
S
L
S
L
G
D
R
G
Site 2
S10
T
T
G
L
E
S
L
S
L
G
D
R
G
A
A
Site 3
T19
G
D
R
G
A
A
P
T
V
T
S
S
E
R
L
Site 4
S22
G
A
A
P
T
V
T
S
S
E
R
L
V
P
D
Site 5
S23
A
A
P
T
V
T
S
S
E
R
L
V
P
D
P
Site 6
S58
E
Q
M
M
I
K
R
S
S
E
C
N
P
L
L
Site 7
S59
Q
M
M
I
K
R
S
S
E
C
N
P
L
L
Q
Site 8
S71
L
L
Q
E
P
I
A
S
A
Q
F
G
A
T
A
Site 9
T80
Q
F
G
A
T
A
G
T
E
C
R
K
S
V
P
Site 10
S85
A
G
T
E
C
R
K
S
V
P
C
G
W
E
R
Site 11
T102
K
Q
R
L
F
G
K
T
A
G
R
F
D
V
Y
Site 12
Y109
T
A
G
R
F
D
V
Y
F
I
S
P
Q
G
L
Site 13
S112
R
F
D
V
Y
F
I
S
P
Q
G
L
K
F
R
Site 14
S120
P
Q
G
L
K
F
R
S
K
S
S
L
A
N
Y
Site 15
S122
G
L
K
F
R
S
K
S
S
L
A
N
Y
L
H
Site 16
S123
L
K
F
R
S
K
S
S
L
A
N
Y
L
H
K
Site 17
Y127
S
K
S
S
L
A
N
Y
L
H
K
N
G
E
T
Site 18
S135
L
H
K
N
G
E
T
S
L
K
P
E
D
F
D
Site 19
T144
K
P
E
D
F
D
F
T
V
L
S
K
R
G
I
Site 20
S147
D
F
D
F
T
V
L
S
K
R
G
I
K
S
R
Site 21
S153
L
S
K
R
G
I
K
S
R
Y
K
D
C
S
M
Site 22
Y155
K
R
G
I
K
S
R
Y
K
D
C
S
M
A
A
Site 23
S159
K
S
R
Y
K
D
C
S
M
A
A
L
T
S
H
Site 24
S165
C
S
M
A
A
L
T
S
H
L
Q
N
Q
S
N
Site 25
S193
K
D
V
F
M
P
P
S
S
S
S
E
L
Q
E
Site 26
S194
D
V
F
M
P
P
S
S
S
S
E
L
Q
E
S
Site 27
S195
V
F
M
P
P
S
S
S
S
E
L
Q
E
S
R
Site 28
S196
F
M
P
P
S
S
S
S
E
L
Q
E
S
R
G
Site 29
S201
S
S
S
E
L
Q
E
S
R
G
L
S
N
F
T
Site 30
S205
L
Q
E
S
R
G
L
S
N
F
T
S
T
H
L
Site 31
S209
R
G
L
S
N
F
T
S
T
H
L
L
L
K
E
Site 32
T246
K
G
I
P
I
K
K
T
K
K
G
C
R
K
S
Site 33
S253
T
K
K
G
C
R
K
S
C
S
G
F
V
Q
S
Site 34
S255
K
G
C
R
K
S
C
S
G
F
V
Q
S
D
S
Site 35
S260
S
C
S
G
F
V
Q
S
D
S
K
R
E
S
V
Site 36
S262
S
G
F
V
Q
S
D
S
K
R
E
S
V
C
N
Site 37
S266
Q
S
D
S
K
R
E
S
V
C
N
K
A
D
A
Site 38
S282
S
E
P
V
A
Q
K
S
Q
L
D
R
T
V
C
Site 39
T287
Q
K
S
Q
L
D
R
T
V
C
I
S
D
A
G
Site 40
S301
G
A
C
G
E
T
L
S
V
T
S
E
E
N
S
Site 41
S308
S
V
T
S
E
E
N
S
L
V
K
K
K
E
R
Site 42
S316
L
V
K
K
K
E
R
S
L
S
S
G
S
N
F
Site 43
S318
K
K
K
E
R
S
L
S
S
G
S
N
F
C
S
Site 44
S319
K
K
E
R
S
L
S
S
G
S
N
F
C
S
E
Site 45
S321
E
R
S
L
S
S
G
S
N
F
C
S
E
Q
K
Site 46
S325
S
S
G
S
N
F
C
S
E
Q
K
T
S
G
I
Site 47
T329
N
F
C
S
E
Q
K
T
S
G
I
I
N
K
F
Site 48
S338
G
I
I
N
K
F
C
S
A
K
D
S
E
H
N
Site 49
S342
K
F
C
S
A
K
D
S
E
H
N
E
K
Y
E
Site 50
Y348
D
S
E
H
N
E
K
Y
E
D
T
F
L
E
S
Site 51
T351
H
N
E
K
Y
E
D
T
F
L
E
S
E
E
I
Site 52
T373
E
R
K
E
H
L
H
T
D
I
L
K
R
G
S
Site 53
S380
T
D
I
L
K
R
G
S
E
M
D
N
N
C
S
Site 54
S387
S
E
M
D
N
N
C
S
P
T
R
K
D
F
T
Site 55
T394
S
P
T
R
K
D
F
T
G
E
K
I
F
Q
E
Site 56
T403
E
K
I
F
Q
E
D
T
I
P
R
T
Q
I
E
Site 57
T414
T
Q
I
E
R
R
K
T
S
L
Y
F
S
S
K
Site 58
S415
Q
I
E
R
R
K
T
S
L
Y
F
S
S
K
Y
Site 59
Y417
E
R
R
K
T
S
L
Y
F
S
S
K
Y
N
K
Site 60
S419
R
K
T
S
L
Y
F
S
S
K
Y
N
K
E
A
Site 61
Y422
S
L
Y
F
S
S
K
Y
N
K
E
A
L
S
P
Site 62
S428
K
Y
N
K
E
A
L
S
P
P
R
R
K
A
F
Site 63
T439
R
K
A
F
K
K
W
T
P
P
R
S
P
F
N
Site 64
S443
K
K
W
T
P
P
R
S
P
F
N
L
V
Q
E
Site 65
Y485
L
W
K
F
L
E
K
Y
P
S
A
E
V
A
R
Site 66
S487
K
F
L
E
K
Y
P
S
A
E
V
A
R
T
A
Site 67
T493
P
S
A
E
V
A
R
T
A
D
W
R
D
V
S
Site 68
S500
T
A
D
W
R
D
V
S
E
L
L
K
P
L
G
Site 69
T515
L
Y
D
L
R
A
K
T
I
V
K
F
S
D
E
Site 70
S520
A
K
T
I
V
K
F
S
D
E
Y
L
T
K
Q
Site 71
Y523
I
V
K
F
S
D
E
Y
L
T
K
Q
W
K
Y
Site 72
Y530
Y
L
T
K
Q
W
K
Y
P
I
E
L
H
G
I
Site 73
Y540
E
L
H
G
I
G
K
Y
G
N
D
S
Y
R
I
Site 74
Y545
G
K
Y
G
N
D
S
Y
R
I
F
C
V
N
E
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation