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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
PAK4
Full Name:
Serine/threonine-protein kinase PAK 4
Alias:
EC 2.7.11.1; P21-activated kinase 4
Type:
Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; PAKB subfamily
Mass (Da):
64072
Number AA:
591
UniProt ID:
O96013
International Prot ID:
IPI00014068
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005794
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0005515
GO:0004674
PhosphoSite+
KinaseNET
Biological Process:
GO:0006928
GO:0006468
GO:0007165
Phosida
TranscriptoNet
STRING
Kinexus Products
p21-activated kinase 4 S474 phosphosite-specific antibody AB-PK752#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK752#PAK4Subtide - PAK4 protein kinase substrate peptide - Powder PE-01BIM95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01BIM95#p21-activated kinase 4 (R471-G477, human) pS474 phosphopeptide - Powder PE-04AED99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AED99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
T23
N
F
E
H
R
V
H
T
G
F
D
Q
H
E
Q
Site 2
T33
D
Q
H
E
Q
K
F
T
G
L
P
R
Q
W
Q
Site 3
S41
G
L
P
R
Q
W
Q
S
L
I
E
E
S
A
R
Site 4
S46
W
Q
S
L
I
E
E
S
A
R
R
P
K
P
L
Site 5
T69
I
Q
P
G
A
P
K
T
I
V
R
G
S
K
G
Site 6
S74
P
K
T
I
V
R
G
S
K
G
A
K
D
G
A
Site 7
S93
L
D
E
F
E
N
M
S
V
T
R
S
N
S
L
Site 8
T95
E
F
E
N
M
S
V
T
R
S
N
S
L
R
R
Site 9
S97
E
N
M
S
V
T
R
S
N
S
L
R
R
D
S
Site 10
S99
M
S
V
T
R
S
N
S
L
R
R
D
S
P
P
Site 11
S104
S
N
S
L
R
R
D
S
P
P
P
P
A
R
A
Site 12
T123
G
M
P
E
E
P
A
T
T
A
R
G
G
P
G
Site 13
T124
M
P
E
E
P
A
T
T
A
R
G
G
P
G
K
Site 14
S134
G
G
P
G
K
A
G
S
R
G
R
F
A
G
H
Site 15
S142
R
G
R
F
A
G
H
S
E
A
G
G
G
S
G
Site 16
S148
H
S
E
A
G
G
G
S
G
D
R
R
R
A
G
Site 17
S162
G
P
E
K
R
P
K
S
S
R
E
G
S
G
G
Site 18
S163
P
E
K
R
P
K
S
S
R
E
G
S
G
G
P
Site 19
S167
P
K
S
S
R
E
G
S
G
G
P
Q
E
S
S
Site 20
S173
G
S
G
G
P
Q
E
S
S
R
D
K
R
P
L
Site 21
S174
S
G
G
P
Q
E
S
S
R
D
K
R
P
L
S
Site 22
S181
S
R
D
K
R
P
L
S
G
P
D
V
G
T
P
Site 23
T187
L
S
G
P
D
V
G
T
P
Q
P
A
G
L
A
Site 24
S195
P
Q
P
A
G
L
A
S
G
A
K
L
A
A
G
Site 25
T207
A
A
G
R
P
F
N
T
Y
P
R
A
D
T
D
Site 26
Y208
A
G
R
P
F
N
T
Y
P
R
A
D
T
D
H
Site 27
T213
N
T
Y
P
R
A
D
T
D
H
P
S
R
G
A
Site 28
S217
R
A
D
T
D
H
P
S
R
G
A
Q
G
E
P
Site 29
S242
G
G
L
A
I
P
Q
S
S
S
S
S
S
R
P
Site 30
S243
G
L
A
I
P
Q
S
S
S
S
S
S
R
P
P
Site 31
S244
L
A
I
P
Q
S
S
S
S
S
S
R
P
P
T
Site 32
S245
A
I
P
Q
S
S
S
S
S
S
R
P
P
T
R
Site 33
S246
I
P
Q
S
S
S
S
S
S
R
P
P
T
R
A
Site 34
S247
P
Q
S
S
S
S
S
S
R
P
P
T
R
A
R
Site 35
T251
S
S
S
S
R
P
P
T
R
A
R
G
A
P
S
Site 36
S258
T
R
A
R
G
A
P
S
P
G
V
L
G
P
H
Site 37
S267
G
V
L
G
P
H
A
S
E
P
Q
L
A
P
P
Site 38
T277
Q
L
A
P
P
A
C
T
P
A
A
P
A
V
P
Site 39
S291
P
G
P
P
G
P
R
S
P
Q
R
E
P
Q
R
Site 40
S300
Q
R
E
P
Q
R
V
S
H
E
Q
F
R
A
A
Site 41
S319
V
D
P
G
D
P
R
S
Y
L
D
N
F
I
K
Site 42
Y320
D
P
G
D
P
R
S
Y
L
D
N
F
I
K
I
Site 43
S342
V
C
I
A
T
V
R
S
S
G
K
L
V
A
V
Site 44
Y373
E
V
V
I
M
R
D
Y
Q
H
E
N
V
V
E
Site 45
S384
N
V
V
E
M
Y
N
S
Y
L
V
G
D
E
L
Site 46
T408
G
A
L
T
D
I
V
T
H
T
R
M
N
E
E
Site 47
S443
V
I
H
R
D
I
K
S
D
S
I
L
L
T
H
Site 48
S445
H
R
D
I
K
S
D
S
I
L
L
T
H
D
G
Site 49
T449
K
S
D
S
I
L
L
T
H
D
G
R
V
K
L
Site 50
S457
H
D
G
R
V
K
L
S
D
F
G
F
C
A
Q
Site 51
S474
K
E
V
P
R
R
K
S
L
V
G
T
P
Y
W
p21-activated kinase 4 S474 phosphosite-specific antibody AB-PK752#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK752#p21-activated kinase 4 (R471-G477, human) pS474 phosphopeptide - Powder PE-04AED99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AED99
Site 52
T478
R
R
K
S
L
V
G
T
P
Y
W
M
A
P
E
Site 53
Y480
K
S
L
V
G
T
P
Y
W
M
A
P
E
L
I
Site 54
S488
W
M
A
P
E
L
I
S
R
L
P
Y
G
P
E
Site 55
Y492
E
L
I
S
R
L
P
Y
G
P
E
V
D
I
W
Site 56
Y515
M
V
D
G
E
P
P
Y
F
N
E
P
P
L
K
Site 57
S542
L
K
N
L
H
K
V
S
P
S
L
K
G
F
L
Site 58
S544
N
L
H
K
V
S
P
S
L
K
G
F
L
D
R
Site 59
T562
R
D
P
A
Q
R
A
T
A
A
E
L
L
K
H
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation