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Protein Info
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Short Name:
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A-Raf
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Full Name:
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Serine/threonine-protein kinase A-Raf
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Alias:
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ARAF; A-RAF; ARAF1; A-raf-1; EC 2.7.11.1; Kinase A-Raf; PKS; PKS2; Proto-oncogene Pks; Raf; V-raf murine sarcoma 3611 viral oncogene
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Type:
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EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; RAF family
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Mass (Da):
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67585
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Number AA:
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606
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UniProt ID:
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P10398
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International Prot ID:
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IPI00020578
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Sequence:
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Retrieve full protein sequence
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GO Terms
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External Links
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Internal Links
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Cellular Component:
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Uniprot
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OncoNet
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Molecular Function:
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GO:0005524
GO:0005515
GO:0004674
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PhosphoSite+
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KinaseNET
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Biological Process:
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GO:0007242
GO:0006468
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Phosida
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TranscriptoNet
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STRING
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Kinexus Products
A-Raf proto-oncogene serine/threonine-protein kinase pan-specific antibody AB-NK205#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK205#A-Raf proto-oncogene serine/threonine-protein kinase pan-specific antibody AB-NK205-1#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK205-1#A-Raf proto-oncogene serine/threonine-protein kinase pan-specific antibody AB-NK205-3#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK205-3#A-Raf proto-oncogene serine/threonine-protein kinase pan-specific antibody AB-NK205-3P#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK205-3P#A-Raf proto-oncogene serine/threonine-protein kinase pan-specific antibody AB-NK205-4#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK205-4#A-Raf proto-oncogene serine/threonine-protein kinase pan-specific antibody AB-NK205-4P#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK205-4P#A-Raf proto-oncogene serine/threonine-protein kinase pan-specific antibody AB-NK205-5#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK205-5#A-Raf proto-oncogene serine/threonine-protein kinase Y302 phosphosite-specific antibody AB-PK500#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK500#A-Raf proto-oncogene serine/threonine-protein kinase (G11-L25, human) peptide - Powder PE-01ASE95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ASE95#A-Raf proto-oncogene serine/threonine-protein kinase (G11-L25, human) peptide - Powder PE-01ASE95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ASE95#A-Raf proto-oncogene serine/threonine-protein kinase (G67-L81, human) peptide - Powder PE-01ASF80#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ASF80#A-Raf proto-oncogene serine/threonine-protein kinase (G67-L81, human) peptide - Powder PE-01ASF80#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ASF80#A-Raf proto-oncogene serine/threonine-protein kinase (G300-K314, human) peptide - Powder PE-01ASG99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ASG99#A-Raf proto-oncogene serine/threonine-protein kinase (G300-K314, human) peptide - Powder PE-01ASG99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ASG99#A-Raf proto-oncogene serine/threonine-protein kinase (S299-V305, human) pY302 phosphopeptide - Powder PE-04AFW99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AFW99#A-Raf proto-oncogene serine/threonine-protein kinase (S299-V305, human) pY302 phosphopeptide - Powder PE-04AYA00#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AYA00
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Info Box
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A variety of parameters were considered in the selection of putative P-sites.
Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score
is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr
or P-Tyr sites. The Maximum KInase Score provides the calculated score for
highest match of 500 human protein kinases for the amino acid sequence
surrounding the target P-site as determined with Kinase Substrate Predictor V2.
The Sum KInase Score provides the additive sum of the positive individual Kinase
Substrate Predictor V2 scores from 500 human protein kinases. The Conservation
Score is the average of the percent similarity of the human P-site with the
equivalent P-site in 20 other diverse species. Click the coloured buttons below
to retrieve this and other information in the Info Box, or click the orange
buttons for relevant links. |
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Phosphosites | |
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| | | -7 | -6 | -5 | -4 | -3 | -2 | -1 | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | | Expt. conf. | Effect | Kinase | PPase | Kinexus Products | | Ref. | Evol. | Kinase Pred. | P-site Match |
| Site 1 | S15 | P | A | N | G | A | E | P | S | R | A | V | G | T | V | K | | | | | | | | | | | |
| Site 2 | T20 | E | P | S | R | A | V | G | T | V | K | V | Y | L | P | N | | | | | | | | | | | |
| Site 3 | Y24 | A | V | G | T | V | K | V | Y | L | P | N | K | Q | R | T | | | | | | | | | | | |
| Site 4 | T34 | N | K | Q | R | T | V | V | T | V | R | D | G | M | S | V | | | | | | | | | | | |
| Site 5 | S40 | V | T | V | R | D | G | M | S | V | Y | D | S | L | D | K | | | | | | | | | | | |
| Site 6 | Y42 | V | R | D | G | M | S | V | Y | D | S | L | D | K | A | L | | | | | | | | | | | |
| Site 7 | S44 | D | G | M | S | V | Y | D | S | L | D | K | A | L | K | V | | | | | | | | | | | |
| Site 8 | Y62 | N | Q | D | C | C | V | V | Y | R | L | I | K | G | R | K | | | | | | | | | | | |
| Site 9 | T70 | R | L | I | K | G | R | K | T | V | T | A | W | D | T | A | | | | | | | | | | | |
| Site 10 | T97 | V | L | E | D | V | P | L | T | M | H | N | F | V | R | K | | | | | | | | | | | |
| Site 11 | Y155 | S | T | N | R | Q | Q | F | Y | H | S | V | Q | D | L | S | | | | | | | | | | | |
| Site 12 | S157 | N | R | Q | Q | F | Y | H | S | V | Q | D | L | S | G | G | | | | | | | | | | | |
| Site 13 | S162 | Y | H | S | V | Q | D | L | S | G | G | S | R | Q | H | E | | | | | | | | | | | |
| Site 14 | S172 | S | R | Q | H | E | A | P | S | N | R | P | L | N | E | L | | | | | | | | | | | |
| Site 15 | T181 | R | P | L | N | E | L | L | T | P | Q | G | P | S | P | R | | | | | | | | | | | |
| Site 16 | S186 | L | L | T | P | Q | G | P | S | P | R | T | Q | H | C | D | | | | | | | | | | | |
| Site 17 | T189 | P | Q | G | P | S | P | R | T | Q | H | C | D | P | E | H | | | | | | | | | | | |
| Site 18 | S212 | A | P | L | Q | R | I | R | S | T | S | T | P | N | V | H | | | | | | | | | | | |
| Site 19 | T213 | P | L | Q | R | I | R | S | T | S | T | P | N | V | H | M | | | | | | | | | | | |
| Site 20 | S214 | L | Q | R | I | R | S | T | S | T | P | N | V | H | M | V | | | | | | | | | | | |
| Site 21 | T215 | Q | R | I | R | S | T | S | T | P | N | V | H | M | V | S | | | | | | | | | | | |
| Site 22 | S222 | T | P | N | V | H | M | V | S | T | T | A | P | M | D | S | | | | | | | | | | | |
| Site 23 | T223 | P | N | V | H | M | V | S | T | T | A | P | M | D | S | N | | | | | | | | | | | |
| Site 24 | T224 | N | V | H | M | V | S | T | T | A | P | M | D | S | N | L | | | | | | | | | | | |
| Site 25 | S229 | S | T | T | A | P | M | D | S | N | L | I | Q | L | T | G | | | | | | | | | | | |
| Site 26 | T235 | D | S | N | L | I | Q | L | T | G | Q | S | F | S | T | D | | | | | | | | | | | |
| Site 27 | S240 | Q | L | T | G | Q | S | F | S | T | D | A | A | G | S | R | | | | | | | | | | | |
| Site 28 | S246 | F | S | T | D | A | A | G | S | R | G | G | S | D | G | T | | | | | | | | | | | |
| Site 29 | S250 | A | A | G | S | R | G | G | S | D | G | T | P | R | G | S | | | | | | | | | | | |
| Site 30 | T253 | S | R | G | G | S | D | G | T | P | R | G | S | P | S | P | | | | | | | | | | | |
| Site 31 | S257 | S | D | G | T | P | R | G | S | P | S | P | A | S | V | S | | | | | | | | | | | |
| Site 32 | S259 | G | T | P | R | G | S | P | S | P | A | S | V | S | S | G | | | | | | | | | | | |
| Site 33 | S262 | R | G | S | P | S | P | A | S | V | S | S | G | R | K | S | | | | | | | | | | | |
| Site 34 | S264 | S | P | S | P | A | S | V | S | S | G | R | K | S | P | H | | | | | | | | | | | |
| Site 35 | S265 | P | S | P | A | S | V | S | S | G | R | K | S | P | H | S | | | | | | | | | | | |
| Site 36 | S269 | S | V | S | S | G | R | K | S | P | H | S | K | S | P | A | | | | | | | | | | | |
| Site 37 | S272 | S | G | R | K | S | P | H | S | K | S | P | A | E | Q | R | | | | | | | | | | | |
| Site 38 | S274 | R | K | S | P | H | S | K | S | P | A | E | Q | R | E | R | | | | | | | | | | | |
| Site 39 | S283 | A | E | Q | R | E | R | K | S | L | A | D | D | K | K | K | | | | | | | | | | | |
| Site 40 | Y296 | K | K | V | K | N | L | G | Y | R | D | S | G | Y | Y | W | | | | | | | | | | | |
| Site 41 | S299 | K | N | L | G | Y | R | D | S | G | Y | Y | W | E | V | P | | | | | | | | | | | |
| Site 42 | Y301 | L | G | Y | R | D | S | G | Y | Y | W | E | V | P | P | S | | | | | | | | | | | |
| Site 43 | Y302 | G | Y | R | D | S | G | Y | Y | W | E | V | P | P | S | E | | | | | | A-Raf proto-oncogene serine/threonine-protein kinase Y302 phosphosite-specific antibody AB-PK500#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK500#A-Raf proto-oncogene serine/threonine-protein kinase (S299-V305, human) pY302 phosphopeptide - Powder PE-04AFW99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AFW99#A-Raf proto-oncogene serine/threonine-protein kinase (S299-V305, human) pY302 phosphopeptide - Powder PE-04AYA00#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AYA00 | | | | | |
| Site 44 | S308 | Y | Y | W | E | V | P | P | S | E | V | Q | L | L | K | R | | | | | | | | | | | |
| Site 45 | T318 | Q | L | L | K | R | I | G | T | G | S | F | G | T | V | F | | | | | | | | | | | |
| Site 46 | S320 | L | K | R | I | G | T | G | S | F | G | T | V | F | R | G | | | | | | | | | | | |
| Site 47 | T323 | I | G | T | G | S | F | G | T | V | F | R | G | R | W | H | | | | | | | | | | | |
| Site 48 | S341 | A | V | K | V | L | K | V | S | Q | P | T | A | E | Q | A | | | | | | | | | | | |
| Site 49 | T344 | V | L | K | V | S | Q | P | T | A | E | Q | A | Q | A | F | | | | | | | | | | | |
| Site 50 | T361 | E | M | Q | V | L | R | K | T | R | H | V | N | I | L | L | | | | | | | | | | | |
| Site 51 | T382 | R | P | G | F | A | I | I | T | Q | W | C | E | G | S | S | | | | | | | | | | | |
| Site 52 | T413 | L | I | D | V | A | R | Q | T | A | Q | G | M | D | Y | L | | | | | | | | | | | |
| Site 53 | Y419 | Q | T | A | Q | G | M | D | Y | L | H | A | K | N | I | I | | | | | | | | | | | |
| Site 54 | S432 | I | I | H | R | D | L | K | S | N | N | I | F | L | H | E | | | | | | | | | | | |
| Site 55 | T442 | I | F | L | H | E | G | L | T | V | K | I | G | D | F | G | | | | | | | | | | | |
| Site 56 | T452 | I | G | D | F | G | L | A | T | V | K | T | R | W | S | G | | | | | | | | | | | |
| Site 57 | T455 | F | G | L | A | T | V | K | T | R | W | S | G | A | Q | P | | | | | | | | | | | |
| Site 58 | S458 | A | T | V | K | T | R | W | S | G | A | Q | P | L | E | Q | | | | | | | | | | | |
| Site 59 | S467 | A | Q | P | L | E | Q | P | S | G | S | V | L | W | M | A | | | | | | | | | | | |
| Site 60 | S469 | P | L | E | Q | P | S | G | S | V | L | W | M | A | A | E | | | | | | | | | | | |
| Site 61 | Y486 | R | M | Q | D | P | N | P | Y | S | F | Q | S | D | V | Y | | | | | | | | | | | |
| Site 62 | S487 | M | Q | D | P | N | P | Y | S | F | Q | S | D | V | Y | A | | | | | | | | | | | |
| Site 63 | S510 | M | T | G | S | L | P | Y | S | H | I | G | C | R | D | Q | | | | | | | | | | | |
| Site 64 | Y526 | I | F | M | V | G | R | G | Y | L | S | P | D | L | S | K | | | | | | | | | | | |
| Site 65 | S528 | M | V | G | R | G | Y | L | S | P | D | L | S | K | I | S | | | | | | | | | | | |
| Site 66 | S532 | G | Y | L | S | P | D | L | S | K | I | S | S | N | C | P | | | | | | | | | | | |
| Site 67 | S535 | S | P | D | L | S | K | I | S | S | N | C | P | K | A | M | | | | | | | | | | | |
| Site 68 | S536 | P | D | L | S | K | I | S | S | N | C | P | K | A | M | R | | | | | | | | | | | |
| Site 69 | S547 | K | A | M | R | R | L | L | S | D | C | L | K | F | Q | R | | | | | | | | | | | |
| Site 70 | S573 | T | I | E | L | L | Q | R | S | L | P | K | I | E | R | S | | | | | | | | | | | |
| Site 71 | S580 | S | L | P | K | I | E | R | S | A | S | E | P | S | L | H | | | | | | | | | | | |
| Site 72 | S582 | P | K | I | E | R | S | A | S | E | P | S | L | H | R | T | | | | | | | | | | | |
| Site 73 | S585 | E | R | S | A | S | E | P | S | L | H | R | T | Q | A | D | | | | | | | | | | | |
| Site 74 | T589 | S | E | P | S | L | H | R | T | Q | A | D | E | L | P | A | | | | | | | | | | | |
| Site 75 | S600 | E | L | P | A | C | L | L | S | A | A | R | L | V | P | _ | | | | | | | | | | | |
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Legend
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Confirmed in mammals
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Confirmed in related proteins or other species
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Predicted by Kinexus P-Site Prediction algorithm
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No data/link available
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Link available
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Products available
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2019 Kinexus Bioinformatics Corporation
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