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Updated November 2019
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Protein Info
Short Name:
TOP2A
Full Name:
DNA topoisomerase 2-alpha
Alias:
Alpha isozyme; DNA topoisomerase II; DNA topoisomerase II alpha; DNA topoisomerase II-alpha; P11388-1; TOP2; TOP-2; Topoisomerase (DNA) II alpha 170kDa; TP2A
Type:
EC 5.99.1.3; Isomerase
Mass (Da):
174385
Number AA:
1531
UniProt ID:
P11388
International Prot ID:
IPI00478232
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0009330
GO:0005694
GO:0005737
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0003918
GO:0008094
PhosphoSite+
KinaseNET
Biological Process:
GO:0006266
GO:0006281
GO:0006260
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S4
_
_
_
_
M
E
V
S
P
L
Q
P
V
N
E
Site 2
S29
E
D
A
K
K
R
L
S
V
E
R
I
Y
Q
K
Site 3
Y34
R
L
S
V
E
R
I
Y
Q
K
K
T
Q
L
E
Site 4
Y64
V
T
Q
Q
M
W
V
Y
D
E
D
V
G
I
N
Site 5
Y72
D
E
D
V
G
I
N
Y
R
E
V
T
F
V
P
Site 6
T76
G
I
N
Y
R
E
V
T
F
V
P
G
L
Y
K
Site 7
Y82
V
T
F
V
P
G
L
Y
K
I
F
D
E
I
L
Site 8
S103
K
Q
R
D
P
K
M
S
C
I
R
V
T
I
D
Site 9
T108
K
M
S
C
I
R
V
T
I
D
P
E
N
N
L
Site 10
S149
F
G
Q
L
L
T
S
S
N
Y
D
D
D
E
K
Site 11
T159
D
D
D
E
K
K
V
T
G
G
R
N
G
Y
G
Site 12
Y165
V
T
G
G
R
N
G
Y
G
A
K
L
C
N
I
Site 13
T178
N
I
F
S
T
K
F
T
V
E
T
A
S
R
E
Site 14
Y186
V
E
T
A
S
R
E
Y
K
K
M
F
K
Q
T
Site 15
Y214
K
P
F
N
G
E
D
Y
T
C
I
T
F
Q
P
Site 16
T218
G
E
D
Y
T
C
I
T
F
Q
P
D
L
S
K
Site 17
S224
I
T
F
Q
P
D
L
S
K
F
K
M
Q
S
L
Site 18
S230
L
S
K
F
K
M
Q
S
L
D
K
D
I
V
A
Site 19
Y244
A
L
M
V
R
R
A
Y
D
I
A
G
S
T
K
Site 20
S249
R
A
Y
D
I
A
G
S
T
K
D
V
K
V
F
Site 21
T250
A
Y
D
I
A
G
S
T
K
D
V
K
V
F
L
Site 22
S269
L
P
V
K
G
F
R
S
Y
V
D
M
Y
L
K
Site 23
Y270
P
V
K
G
F
R
S
Y
V
D
M
Y
L
K
D
Site 24
Y274
F
R
S
Y
V
D
M
Y
L
K
D
K
L
D
E
Site 25
T282
L
K
D
K
L
D
E
T
G
N
S
L
K
V
I
Site 26
S285
K
L
D
E
T
G
N
S
L
K
V
I
H
E
Q
Site 27
S312
E
K
G
F
Q
Q
I
S
F
V
N
S
I
A
T
Site 28
S316
Q
Q
I
S
F
V
N
S
I
A
T
S
K
G
G
Site 29
S320
F
V
N
S
I
A
T
S
K
G
G
R
H
V
D
Site 30
Y328
K
G
G
R
H
V
D
Y
V
A
D
Q
I
V
T
Site 31
T372
N
A
L
I
E
N
P
T
F
D
S
Q
T
K
E
Site 32
S375
I
E
N
P
T
F
D
S
Q
T
K
E
N
M
T
Site 33
T382
S
Q
T
K
E
N
M
T
L
Q
P
K
S
F
G
Site 34
S387
N
M
T
L
Q
P
K
S
F
G
S
T
C
Q
L
Site 35
S390
L
Q
P
K
S
F
G
S
T
C
Q
L
S
E
K
Site 36
T391
Q
P
K
S
F
G
S
T
C
Q
L
S
E
K
F
Site 37
S452
N
D
A
G
G
R
N
S
T
E
C
T
L
I
L
Site 38
T453
D
A
G
G
R
N
S
T
E
C
T
L
I
L
T
Site 39
T456
G
R
N
S
T
E
C
T
L
I
L
T
E
G
D
Site 40
T467
T
E
G
D
S
A
K
T
L
A
V
S
G
L
G
Site 41
Y481
G
V
V
G
R
D
K
Y
G
V
F
P
L
R
G
Site 42
S497
I
L
N
V
R
E
A
S
H
K
Q
I
M
E
N
Site 43
Y518
I
K
I
V
G
L
Q
Y
K
K
N
Y
E
D
E
Site 44
S527
K
N
Y
E
D
E
D
S
L
K
T
L
R
Y
G
Site 45
T530
E
D
E
D
S
L
K
T
L
R
Y
G
K
I
M
Site 46
T540
Y
G
K
I
M
I
M
T
D
Q
D
Q
D
G
S
Site 47
S547
T
D
Q
D
Q
D
G
S
H
I
K
G
L
L
I
Site 48
Y590
N
K
Q
E
M
A
F
Y
S
L
P
E
F
E
E
Site 49
S591
K
Q
E
M
A
F
Y
S
L
P
E
F
E
E
W
Site 50
S600
P
E
F
E
E
W
K
S
S
T
P
N
H
K
K
Site 51
T602
F
E
E
W
K
S
S
T
P
N
H
K
K
W
K
Site 52
Y612
H
K
K
W
K
V
K
Y
Y
K
G
L
G
T
S
Site 53
Y613
K
K
W
K
V
K
Y
Y
K
G
L
G
T
S
T
Site 54
T618
K
Y
Y
K
G
L
G
T
S
T
S
K
E
A
K
Site 55
S619
Y
Y
K
G
L
G
T
S
T
S
K
E
A
K
E
Site 56
T620
Y
K
G
L
G
T
S
T
S
K
E
A
K
E
Y
Site 57
S621
K
G
L
G
T
S
T
S
K
E
A
K
E
Y
F
Site 58
Y627
T
S
K
E
A
K
E
Y
F
A
D
M
K
R
H
Site 59
Y640
R
H
R
I
Q
F
K
Y
S
G
P
E
D
D
A
Site 60
S650
P
E
D
D
A
A
I
S
L
A
F
S
K
K
Q
Site 61
S654
A
A
I
S
L
A
F
S
K
K
Q
I
D
D
R
Site 62
Y684
L
L
G
L
P
E
D
Y
L
Y
G
Q
T
T
T
Site 63
Y686
G
L
P
E
D
Y
L
Y
G
Q
T
T
T
Y
L
Site 64
Y692
L
Y
G
Q
T
T
T
Y
L
T
Y
N
D
F
I
Site 65
S707
N
K
E
L
I
L
F
S
N
S
D
N
E
R
S
Site 66
S709
E
L
I
L
F
S
N
S
D
N
E
R
S
I
P
Site 67
S714
S
N
S
D
N
E
R
S
I
P
S
M
V
D
G
Site 68
S717
D
N
E
R
S
I
P
S
M
V
D
G
L
K
P
Site 69
T732
G
Q
R
K
V
L
F
T
C
F
K
R
N
D
K
Site 70
S755
A
G
S
V
A
E
M
S
S
Y
H
H
G
E
M
Site 71
S756
G
S
V
A
E
M
S
S
Y
H
H
G
E
M
S
Site 72
Y757
S
V
A
E
M
S
S
Y
H
H
G
E
M
S
L
Site 73
S800
R
L
H
G
G
K
D
S
A
S
P
R
Y
I
F
Site 74
S802
H
G
G
K
D
S
A
S
P
R
Y
I
F
T
M
Site 75
Y805
K
D
S
A
S
P
R
Y
I
F
T
M
L
S
S
Site 76
T825
F
P
P
K
D
D
H
T
L
K
F
L
Y
D
D
Site 77
Y830
D
H
T
L
K
F
L
Y
D
D
N
Q
R
V
E
Site 78
T858
N
G
A
E
G
I
G
T
G
W
S
C
K
I
P
Site 79
S891
E
P
L
P
M
L
P
S
Y
K
N
F
K
G
T
Site 80
Y892
P
L
P
M
L
P
S
Y
K
N
F
K
G
T
I
Site 81
Y907
E
E
L
A
P
N
Q
Y
V
I
S
G
E
V
A
Site 82
T932
E
L
P
V
R
T
W
T
Q
T
Y
K
E
Q
V
Site 83
T934
P
V
R
T
W
T
Q
T
Y
K
E
Q
V
L
E
Site 84
T950
M
L
N
G
T
E
K
T
P
P
L
I
T
D
Y
Site 85
T955
E
K
T
P
P
L
I
T
D
Y
R
E
Y
H
T
Site 86
Y957
T
P
P
L
I
T
D
Y
R
E
Y
H
T
D
T
Site 87
Y960
L
I
T
D
Y
R
E
Y
H
T
D
T
T
V
K
Site 88
T964
Y
R
E
Y
H
T
D
T
T
V
K
F
V
V
K
Site 89
T965
R
E
Y
H
T
D
T
T
V
K
F
V
V
K
M
Site 90
T1014
G
C
L
K
K
Y
D
T
V
L
D
I
L
R
D
Site 91
Y1029
F
F
E
L
R
L
K
Y
Y
G
L
R
K
E
W
Site 92
Y1030
F
E
L
R
L
K
Y
Y
G
L
R
K
E
W
L
Site 93
Y1082
K
V
L
I
Q
R
G
Y
D
S
D
P
V
K
A
Site 94
S1084
L
I
Q
R
G
Y
D
S
D
P
V
K
A
W
K
Site 95
S1106
D
E
E
E
N
E
E
S
D
N
E
K
E
T
E
Site 96
S1115
N
E
K
E
T
E
K
S
D
S
V
T
D
S
G
Site 97
S1117
K
E
T
E
K
S
D
S
V
T
D
S
G
P
T
Site 98
T1119
T
E
K
S
D
S
V
T
D
S
G
P
T
F
N
Site 99
S1121
K
S
D
S
V
T
D
S
G
P
T
F
N
Y
L
Site 100
T1124
S
V
T
D
S
G
P
T
F
N
Y
L
L
D
M
Site 101
Y1127
D
S
G
P
T
F
N
Y
L
L
D
M
P
L
W
Site 102
Y1135
L
L
D
M
P
L
W
Y
L
T
K
E
K
K
D
Site 103
T1157
E
K
E
Q
E
L
D
T
L
K
R
K
S
P
S
Site 104
S1162
L
D
T
L
K
R
K
S
P
S
D
L
W
K
E
Site 105
S1164
T
L
K
R
K
S
P
S
D
L
W
K
E
D
L
Site 106
T1173
L
W
K
E
D
L
A
T
F
I
E
E
L
E
A
Site 107
T1205
G
K
A
K
G
K
K
T
Q
M
A
E
V
L
P
Site 108
S1213
Q
M
A
E
V
L
P
S
P
R
G
Q
R
V
I
Site 109
T1224
Q
R
V
I
P
R
I
T
I
E
M
K
A
E
A
Site 110
T1244
K
K
I
K
N
E
N
T
E
G
S
P
Q
E
D
Site 111
S1247
K
N
E
N
T
E
G
S
P
Q
E
D
G
V
E
Site 112
T1274
K
R
E
P
G
T
K
T
K
K
Q
T
T
L
A
Site 113
T1279
T
K
T
K
K
Q
T
T
L
A
F
K
P
I
K
Site 114
S1295
G
K
K
R
N
P
W
S
D
S
E
S
D
R
S
Site 115
S1297
K
R
N
P
W
S
D
S
E
S
D
R
S
S
D
Site 116
S1299
N
P
W
S
D
S
E
S
D
R
S
S
D
E
S
Site 117
S1302
S
D
S
E
S
D
R
S
S
D
E
S
N
F
D
Site 118
S1303
D
S
E
S
D
R
S
S
D
E
S
N
F
D
V
Site 119
S1306
S
D
R
S
S
D
E
S
N
F
D
V
P
P
R
Site 120
T1315
F
D
V
P
P
R
E
T
E
P
R
R
A
A
T
Site 121
T1322
T
E
P
R
R
A
A
T
K
T
K
F
T
M
D
Site 122
T1324
P
R
R
A
A
T
K
T
K
F
T
M
D
L
D
Site 123
T1327
A
A
T
K
T
K
F
T
M
D
L
D
S
D
E
Site 124
S1332
K
F
T
M
D
L
D
S
D
E
D
F
S
D
F
Site 125
S1337
L
D
S
D
E
D
F
S
D
F
D
E
K
T
D
Site 126
T1343
F
S
D
F
D
E
K
T
D
D
E
D
F
V
P
Site 127
S1351
D
D
E
D
F
V
P
S
D
A
S
P
P
K
T
Site 128
S1354
D
F
V
P
S
D
A
S
P
P
K
T
K
T
S
Site 129
T1358
S
D
A
S
P
P
K
T
K
T
S
P
K
L
S
Site 130
T1360
A
S
P
P
K
T
K
T
S
P
K
L
S
N
K
Site 131
S1361
S
P
P
K
T
K
T
S
P
K
L
S
N
K
E
Site 132
S1365
T
K
T
S
P
K
L
S
N
K
E
L
K
P
Q
Site 133
S1374
K
E
L
K
P
Q
K
S
V
V
S
D
L
E
A
Site 134
S1377
K
P
Q
K
S
V
V
S
D
L
E
A
D
D
V
Site 135
S1387
E
A
D
D
V
K
G
S
V
P
L
S
S
S
P
Site 136
S1391
V
K
G
S
V
P
L
S
S
S
P
P
A
T
H
Site 137
S1392
K
G
S
V
P
L
S
S
S
P
P
A
T
H
F
Site 138
S1393
G
S
V
P
L
S
S
S
P
P
A
T
H
F
P
Site 139
T1397
L
S
S
S
P
P
A
T
H
F
P
D
E
T
E
Site 140
T1403
A
T
H
F
P
D
E
T
E
I
T
N
P
V
P
Site 141
T1406
F
P
D
E
T
E
I
T
N
P
V
P
K
K
N
Site 142
T1415
P
V
P
K
K
N
V
T
V
K
K
T
A
A
K
Site 143
T1419
K
N
V
T
V
K
K
T
A
A
K
S
Q
S
S
Site 144
S1423
V
K
K
T
A
A
K
S
Q
S
S
T
S
T
T
Site 145
T1429
K
S
Q
S
S
T
S
T
T
G
A
K
K
R
A
Site 146
T1430
S
Q
S
S
T
S
T
T
G
A
K
K
R
A
A
Site 147
S1449
K
R
D
P
A
L
N
S
G
V
S
Q
K
P
D
Site 148
S1452
P
A
L
N
S
G
V
S
Q
K
P
D
P
A
K
Site 149
T1460
Q
K
P
D
P
A
K
T
K
N
R
R
K
R
K
Site 150
S1469
N
R
R
K
R
K
P
S
T
S
D
D
S
D
S
Site 151
T1470
R
R
K
R
K
P
S
T
S
D
D
S
D
S
N
Site 152
S1471
R
K
R
K
P
S
T
S
D
D
S
D
S
N
F
Site 153
S1474
K
P
S
T
S
D
D
S
D
S
N
F
E
K
I
Site 154
S1476
S
T
S
D
D
S
D
S
N
F
E
K
I
V
S
Site 155
S1483
S
N
F
E
K
I
V
S
K
A
V
T
S
K
K
Site 156
T1487
K
I
V
S
K
A
V
T
S
K
K
S
K
G
E
Site 157
S1491
K
A
V
T
S
K
K
S
K
G
E
S
D
D
F
Site 158
S1495
S
K
K
S
K
G
E
S
D
D
F
H
M
D
F
Site 159
S1504
D
F
H
M
D
F
D
S
A
V
A
P
R
A
K
Site 160
S1512
A
V
A
P
R
A
K
S
V
R
A
K
K
P
I
Site 161
Y1521
R
A
K
K
P
I
K
Y
L
E
E
S
D
E
D
Site 162
S1525
P
I
K
Y
L
E
E
S
D
E
D
D
L
F
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation