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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
PKM2
Full Name:
Pyruvate kinase isozymes M1/M2
Alias:
CTHBP; Cytosolic thyroid hormone-binding protein; EC 2.7.1.40; KPYM; PKM; Pyruvate kinase 2/3; Pyruvate kinase M; Pyruvate kinase muscle isozyme; Pyruvate kinase, M2 isozyme
Type:
Nucleotide Metabolism - purine; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase (non-protein); Carbohydrate Metabolism - pyruvate; EC 2.7.1.40
Mass (Da):
57937
Number AA:
531
UniProt ID:
P14618
International Prot ID:
IPI00479186
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005737
GO:0005829
GO:0044424
Uniprot
OncoNet
Molecular Function:
GO:0000287
GO:0003824
GO:0004743
PhosphoSite+
KinaseNET
Biological Process:
GO:0005975
GO:0005996
GO:0006006
Phosida
TranscriptoNet
STRING
Kinexus Products
Pyruvate kinase isozymes M1/M2 Y390 phosphosite-specific antibody AB-PN529#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN529#Pyruvate kinase isozymes M1/M2 (A387-Q393, human) pY390 phosphopeptide - Powder PE-04AHE95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AHE95
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S37
M
C
R
L
D
I
D
S
P
P
I
T
A
R
N
Site 2
T41
D
I
D
S
P
P
I
T
A
R
N
T
G
I
I
Site 3
T45
P
P
I
T
A
R
N
T
G
I
I
C
T
I
G
Site 4
T50
R
N
T
G
I
I
C
T
I
G
P
A
S
R
S
Site 5
S55
I
C
T
I
G
P
A
S
R
S
V
E
T
L
K
Site 6
S57
T
I
G
P
A
S
R
S
V
E
T
L
K
E
M
Site 7
S67
T
L
K
E
M
I
K
S
G
M
N
V
A
R
L
Site 8
S77
N
V
A
R
L
N
F
S
H
G
T
H
E
Y
H
Site 9
Y83
F
S
H
G
T
H
E
Y
H
A
E
T
I
K
N
Site 10
T87
T
H
E
Y
H
A
E
T
I
K
N
V
R
T
A
Site 11
T93
E
T
I
K
N
V
R
T
A
T
E
S
F
A
S
Site 12
T95
I
K
N
V
R
T
A
T
E
S
F
A
S
D
P
Site 13
S97
N
V
R
T
A
T
E
S
F
A
S
D
P
I
L
Site 14
S100
T
A
T
E
S
F
A
S
D
P
I
L
Y
R
P
Site 15
Y105
F
A
S
D
P
I
L
Y
R
P
V
A
V
A
L
Site 16
T114
P
V
A
V
A
L
D
T
K
G
P
E
I
R
T
Site 17
T143
K
G
A
T
L
K
I
T
L
D
N
A
Y
M
E
Site 18
Y148
K
I
T
L
D
N
A
Y
M
E
K
C
D
E
N
Site 19
Y161
E
N
I
L
W
L
D
Y
K
N
I
C
K
V
V
Site 20
S172
C
K
V
V
E
V
G
S
K
I
Y
V
D
D
G
Site 21
Y175
V
E
V
G
S
K
I
Y
V
D
D
G
L
I
S
Site 22
S182
Y
V
D
D
G
L
I
S
L
Q
V
K
Q
K
G
Site 23
T195
K
G
A
D
F
L
V
T
E
V
E
N
G
G
S
Site 24
S202
T
E
V
E
N
G
G
S
L
G
S
K
K
G
V
Site 25
S243
D
V
D
M
V
F
A
S
F
I
R
K
A
S
D
Site 26
S249
A
S
F
I
R
K
A
S
D
V
H
E
V
R
K
Site 27
S269
G
K
N
I
K
I
I
S
K
I
E
N
H
E
G
Site 28
S287
F
D
E
I
L
E
A
S
D
G
I
M
V
A
R
Site 29
T328
G
K
P
V
I
C
A
T
Q
M
L
E
S
M
I
Site 30
S333
C
A
T
Q
M
L
E
S
M
I
K
K
P
R
P
Site 31
T341
M
I
K
K
P
R
P
T
R
A
E
G
S
D
V
Site 32
S362
G
A
D
C
I
M
L
S
G
E
T
A
K
G
D
Site 33
Y370
G
E
T
A
K
G
D
Y
P
L
E
A
V
R
M
Site 34
Y390
R
E
A
E
A
A
I
Y
H
L
Q
L
F
E
E
Pyruvate kinase isozymes M1/M2 Y390 phosphosite-specific antibody AB-PN529#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN529#Pyruvate kinase isozymes M1/M2 (A387-Q393, human) pY390 phosphopeptide - Powder PE-04AHE95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AHE95
Site 35
T405
L
R
R
L
A
P
I
T
S
D
P
T
E
A
T
Site 36
S406
R
R
L
A
P
I
T
S
D
P
T
E
A
T
A
Site 37
S437
V
L
T
K
S
G
R
S
A
H
Q
V
A
R
Y
Site 38
Y444
S
A
H
Q
V
A
R
Y
R
P
R
A
P
I
I
Site 39
T454
R
A
P
I
I
A
V
T
R
N
P
Q
T
A
R
Site 40
T459
A
V
T
R
N
P
Q
T
A
R
Q
A
H
L
Y
Site 41
Y466
T
A
R
Q
A
H
L
Y
R
G
I
F
P
V
L
Site 42
S519
L
T
G
W
R
P
G
S
G
F
T
N
T
M
R
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation