|
|
|
|
|
|
|
|
|
|
|
|
Protein Info
|
|
|
|
|
|
Short Name:
|
PRKCA
|
|
Full Name:
|
Protein kinase C alpha type
|
|
Alias:
|
EC 2.7.11.13; Kinase PKC-alpha; KPCA; PKC III; PKC-A; PKC-alpha; PKC-III; PRKACA; Protein kinase C, alpha; Protein kinase C, alpha type
|
|
Type:
|
EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Alpha subfamily
|
|
Mass (Da):
|
76764
|
|
Number AA:
|
672
|
|
UniProt ID:
|
P17252
|
|
International Prot ID:
|
IPI00385449
|
|
Sequence:
|
Retrieve full protein sequence
|
|
|
|
|
GO Terms
|
|
External Links
|
Internal Links
|
|
|
|
|
|
Cellular Component:
|
GO:0005829
GO:0019898
GO:0005886
|
Uniprot
|
OncoNet
|
|
Molecular Function:
|
GO:0005524
GO:0005509
GO:0005515
|
PhosphoSite+
|
KinaseNET
|
|
|
Biological Process:
|
GO:0008624
GO:0070555
|
Phosida
|
TranscriptoNet
|
|
|
|
|
STRING
|
Kinexus Products
Protein-serine kinase C alpha pan-specific antibody AB-NK201#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK201#Protein-serine kinase C alpha T497 phosphosite-specific antibody AB-PK763#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK763#Protein-serine kinase C alpha Y195 phosphosite-specific antibody AB-PK764#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK764#CREB1 (123-135) KinSub - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide - Powder PE-01ACP95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ACP95#cAMP-dependent protein kinase inhibitor alpha (C15-I23) peptide - Powder PE-01ADK95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ADK95#PKCtide KinSub - Protein kinase C peptide substrate - Powder PE-01ADL95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ADL95#Syntide-2 KinSub - Syntide-2 protein kinase substrate peptide - Powder PE-01AQB95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01AQB95#PKCA/BSubtide - PKCa (PRKCA) protein kinase substrate peptide - Powder PE-01BIR95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01BIR95#CREB1 (123-135) KinSub, biotinyl. - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide - Powder PE-03AAF90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-03AAF90#PKC peptide, biotinyl. - Protein kinase C protein kinase substrate peptide, biotinylated - Powder PE-03AAK90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-03AAK90#Protein-serine kinase C alpha (T494-G500, human) pT497 phosphopeptide - Powder PE-04AOS99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AOS99#Protein-serine kinase C alpha (S192-L198, human) pY195 phosphopeptide - Powder PE-04AOU99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AOU99
|
|
|
|
|
Info Box
|
|
A variety of parameters were considered in the selection of putative P-sites.
Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score
is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr
or P-Tyr sites. The Maximum KInase Score provides the calculated score for
highest match of 500 human protein kinases for the amino acid sequence
surrounding the target P-site as determined with Kinase Substrate Predictor V2.
The Sum KInase Score provides the additive sum of the positive individual Kinase
Substrate Predictor V2 scores from 500 human protein kinases. The Conservation
Score is the average of the percent similarity of the human P-site with the
equivalent P-site in 20 other diverse species. Click the coloured buttons below
to retrieve this and other information in the Info Box, or click the orange
buttons for relevant links. |
|
| Phosphosites | |
| |
| | | -7 | -6 | -5 | -4 | -3 | -2 | -1 | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | | Expt. conf. | Effect | Kinase | PPase | Kinexus Products | | Ref. | Evol. | Kinase Pred. | P-site Match |
| | Site 1 | S10 | D | V | F | P | G | N | D | S | T | A | S | Q | D | V | A | |  |  | | | | |  | | | |
| | Site 2 | T11 | V | F | P | G | N | D | S | T | A | S | Q | D | V | A | N | | | | | | | | | | | |
| | Site 3 | S13 | P | G | N | D | S | T | A | S | Q | D | V | A | N | R | F | |  | | | | | | | | | |
| | Site 4 | T48 | A | R | F | F | K | Q | P | T | F | C | S | H | C | T | D | | | | | | | | | | | |
| | Site 5 | T83 | K | R | C | H | E | F | V | T | F | S | C | P | G | A | D | | | | | | | | | | | |
| | Site 6 | T95 | G | A | D | K | G | P | D | T | D | D | P | R | S | K | H | | | | | | | | | | | |
| | Site 7 | S100 | P | D | T | D | D | P | R | S | K | H | K | F | K | I | H | | | | | | | | | | | |
| | Site 8 | Y109 | H | K | F | K | I | H | T | Y | G | S | P | T | F | C | D | | | | | | | | | | | |
| | Site 9 | S149 | Q | C | V | I | N | V | P | S | L | C | G | M | D | H | T | | | | | | | | | | | |
| | Site 10 | T156 | S | L | C | G | M | D | H | T | E | K | R | G | R | I | Y | | | | | | | | | | | |
| | Site 11 | Y163 | T | E | K | R | G | R | I | Y | L | K | A | E | V | A | D | | | | | | | | | | | |
| | Site 12 | T176 | A | D | E | K | L | H | V | T | V | R | D | A | K | N | L | | | | | | | | | | | |
| | Site 13 | S192 | P | M | D | P | N | G | L | S | D | P | Y | V | K | L | K | | | | | | | | | | | |
| | Site 14 | Y195 | P | N | G | L | S | D | P | Y | V | K | L | K | L | I | P | | | | | |  Protein-serine kinase C alpha Y195 phosphosite-specific antibody AB-PK764#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK764#Protein-serine kinase C alpha (S192-L198, human) pY195 phosphopeptide - Powder PE-04AOU99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AOU99 | | | | | |
| | Site 15 | S208 | I | P | D | P | K | N | E | S | K | Q | K | T | K | T | I | | | | | | | | | | | |
| | Site 16 | T212 | K | N | E | S | K | Q | K | T | K | T | I | R | S | T | L | | | | | | | | | | | |
| | Site 17 | T214 | E | S | K | Q | K | T | K | T | I | R | S | T | L | N | P | | | | | | | | | | | |
| | Site 18 | T218 | K | T | K | T | I | R | S | T | L | N | P | Q | W | N | E | | | | | | | | | | | |
| | Site 19 | S226 | L | N | P | Q | W | N | E | S | F | T | F | K | L | K | P | | | | | | | | | | | |
| | Site 20 | T228 | P | Q | W | N | E | S | F | T | F | K | L | K | P | S | D | | | | | | | | | | | |
| | Site 21 | S241 | S | D | K | D | R | R | L | S | V | E | I | W | D | W | D | | | | | | | | | | | |
| | Site 22 | S258 | T | R | N | D | F | M | G | S | L | S | F | G | V | S | E | | | | | | | | | | | |
| | Site 23 | Y285 | L | N | Q | E | E | G | E | Y | Y | N | V | P | I | P | E | | | | | | | | | | | |
| | Site 24 | Y286 | N | Q | E | E | G | E | Y | Y | N | V | P | I | P | E | G | | | | | | | | | | | |
| | Site 25 | S319 | P | A | G | N | K | V | I | S | P | S | E | D | R | K | Q | | | | | | | | | | | |
| | Site 26 | S321 | G | N | K | V | I | S | P | S | E | D | R | K | Q | P | S | | | | | | | | | | | |
| | Site 27 | T337 | N | L | D | R | V | K | L | T | D | F | N | F | L | M | V | | | | | | | | | | | |
| | Site 28 | S349 | L | M | V | L | G | K | G | S | F | G | K | V | M | L | A | | | | | | | | | | | |
| | Site 29 | T361 | M | L | A | D | R | K | G | T | E | E | L | Y | A | I | K | | | | | | | | | | | |
| | Site 30 | Y365 | R | K | G | T | E | E | L | Y | A | I | K | I | L | K | K | | | | | | | | | | | |
| | Site 31 | T401 | L | D | K | P | P | F | L | T | Q | L | H | S | C | F | Q | | | | | | | | | | | |
| | Site 32 | S405 | P | F | L | T | Q | L | H | S | C | F | Q | T | V | D | R | | | | | | | | | | | |
| | Site 33 | Y427 | V | N | G | G | D | L | M | Y | H | I | Q | Q | V | G | K | | | | | | | | | | | |
| | Site 34 | S473 | L | D | N | V | M | L | D | S | E | G | H | I | K | I | A | | | | | | | | | | | |
| | Site 35 | T494 | E | H | M | M | D | G | V | T | T | R | T | F | C | G | T | | | | | | | | | | | |
| | Site 36 | T495 | H | M | M | D | G | V | T | T | R | T | F | C | G | T | P | | | | | | | | | | | |
| | Site 37 | T497 | M | D | G | V | T | T | R | T | F | C | G | T | P | D | Y | | | | | | Protein-serine kinase C alpha T497 phosphosite-specific antibody AB-PK763#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK763#Protein-serine kinase C alpha (T494-G500, human) pT497 phosphopeptide - Powder PE-04AOS99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AOS99 | | | | | |
| | Site 38 | T501 | T | T | R | T | F | C | G | T | P | D | Y | I | A | P | E | | | | | | | | | | | |
| | Site 39 | Y504 | T | F | C | G | T | P | D | Y | I | A | P | E | I | I | A | | | | | | | | | | | |
| | Site 40 | Y512 | I | A | P | E | I | I | A | Y | Q | P | Y | G | K | S | V | | | | | | | | | | | |
| | Site 41 | Y515 | E | I | I | A | Y | Q | P | Y | G | K | S | V | D | W | W | | | | | | | | | | | |
| | Site 42 | S549 | D | E | D | E | L | F | Q | S | I | M | E | H | N | V | S | | | | | | | | | | | |
| | Site 43 | S556 | S | I | M | E | H | N | V | S | Y | P | K | S | L | S | K | | | | | | | | | | | |
| | Site 44 | Y557 | I | M | E | H | N | V | S | Y | P | K | S | L | S | K | E | | | | | | | | | | | |
| | Site 45 | S560 | H | N | V | S | Y | P | K | S | L | S | K | E | A | V | S | | | | | | | | | | | |
| | Site 46 | S562 | V | S | Y | P | K | S | L | S | K | E | A | V | S | I | C | | | | | | | | | | | |
| | Site 47 | T574 | S | I | C | K | G | L | M | T | K | H | P | A | K | R | L | | | | | | | | | | | |
| | Site 48 | T631 | E | N | F | D | K | F | F | T | R | G | Q | P | V | L | T | | | | | | | | | | | |
| | Site 49 | T638 | T | R | G | Q | P | V | L | T | P | P | D | Q | L | V | I | | | | | | | | | | | |
| | Site 50 | S651 | V | I | A | N | I | D | Q | S | D | F | E | G | F | S | Y | | | | | | | | | | | |
| | Site 51 | S657 | Q | S | D | F | E | G | F | S | Y | V | N | P | Q | F | V | | | | | | | | | | | |
| | Site 52 | Y658 | S | D | F | E | G | F | S | Y | V | N | P | Q | F | V | H | | | | | | | | | | | |
| | Site 53 | S670 | F | V | H | P | I | L | Q | S | A | V | _ | _ | _ | _ | _ | | | | | | | | | | | |
|
|
|
|
|
|
|
|
Legend
|
|
|
|
Confirmed in mammals
|
|
Confirmed in related proteins or other species
|
|
|
Predicted by Kinexus P-Site Prediction algorithm
|
|
|
No data/link available
|
|
|
Link available
|
|
|
Products available
|
|
|
|
|
|
|
|
|
|
2019 Kinexus Bioinformatics Corporation
|
|