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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
CAST
Full Name:
Calpastatin
Alias:
Calpain inhibitor; ICAL; Sperm BS-17 component
Type:
Inhibitor protein
Mass (Da):
76573
Number AA:
708
UniProt ID:
P20810
International Prot ID:
IPI00305750
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
Uniprot
OncoNet
Molecular Function:
GO:0010859
GO:0005515
PhosphoSite+
KinaseNET
Biological Process:
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S12
E
T
K
A
I
P
V
S
Q
Q
M
E
G
P
H
Site 2
S39
K
T
E
P
E
K
K
S
Q
S
T
K
L
S
V
Site 3
S41
E
P
E
K
K
S
Q
S
T
K
L
S
V
V
H
Site 4
S45
K
S
Q
S
T
K
L
S
V
V
H
E
K
K
S
Site 5
S52
S
V
V
H
E
K
K
S
Q
E
G
K
P
K
E
Site 6
T61
E
G
K
P
K
E
H
T
E
P
K
S
L
P
K
Site 7
S65
K
E
H
T
E
P
K
S
L
P
K
Q
A
S
D
Site 8
S71
K
S
L
P
K
Q
A
S
D
T
G
S
N
D
A
Site 9
T73
L
P
K
Q
A
S
D
T
G
S
N
D
A
H
N
Site 10
S75
K
Q
A
S
D
T
G
S
N
D
A
H
N
K
K
Site 11
S87
N
K
K
A
V
S
R
S
A
E
Q
Q
P
S
E
Site 12
S93
R
S
A
E
Q
Q
P
S
E
K
S
T
E
P
K
Site 13
S96
E
Q
Q
P
S
E
K
S
T
E
P
K
T
K
P
Site 14
T97
Q
Q
P
S
E
K
S
T
E
P
K
T
K
P
Q
Site 15
T101
E
K
S
T
E
P
K
T
K
P
Q
D
M
I
S
Site 16
S108
T
K
P
Q
D
M
I
S
A
G
G
E
S
V
A
Site 17
S133
D
K
K
K
E
K
K
S
L
T
P
A
V
P
V
Site 18
T135
K
K
E
K
K
S
L
T
P
A
V
P
V
E
S
Site 19
S142
T
P
A
V
P
V
E
S
K
P
D
K
P
S
G
Site 20
S148
E
S
K
P
D
K
P
S
G
K
S
G
M
D
A
Site 21
T163
A
L
D
D
L
I
D
T
L
G
G
P
E
E
T
Site 22
T175
E
E
T
E
E
E
N
T
T
Y
T
G
P
E
V
Site 23
T176
E
T
E
E
E
N
T
T
Y
T
G
P
E
V
S
Site 24
Y177
T
E
E
E
N
T
T
Y
T
G
P
E
V
S
D
Site 25
S183
T
Y
T
G
P
E
V
S
D
P
M
S
S
T
Y
Site 26
S187
P
E
V
S
D
P
M
S
S
T
Y
I
E
E
L
Site 27
S188
E
V
S
D
P
M
S
S
T
Y
I
E
E
L
G
Site 28
T189
V
S
D
P
M
S
S
T
Y
I
E
E
L
G
K
Site 29
Y190
S
D
P
M
S
S
T
Y
I
E
E
L
G
K
R
Site 30
T200
E
L
G
K
R
E
V
T
I
P
P
K
Y
R
E
Site 31
Y205
E
V
T
I
P
P
K
Y
R
E
L
L
A
K
K
Site 32
T216
L
A
K
K
E
G
I
T
G
P
P
A
D
S
S
Site 33
S222
I
T
G
P
P
A
D
S
S
K
P
I
G
P
D
Site 34
S223
T
G
P
P
A
D
S
S
K
P
I
G
P
D
D
Site 35
S236
D
D
A
I
D
A
L
S
S
D
F
T
C
G
S
Site 36
S237
D
A
I
D
A
L
S
S
D
F
T
C
G
S
P
Site 37
T240
D
A
L
S
S
D
F
T
C
G
S
P
T
A
A
Site 38
S243
S
S
D
F
T
C
G
S
P
T
A
A
G
K
K
Site 39
T245
D
F
T
C
G
S
P
T
A
A
G
K
K
T
E
Site 40
S256
K
K
T
E
K
E
E
S
T
E
V
L
K
A
Q
Site 41
T267
L
K
A
Q
S
A
G
T
V
R
S
A
A
P
P
Site 42
S287
K
V
E
K
D
T
M
S
D
Q
A
L
E
A
L
Site 43
S295
D
Q
A
L
E
A
L
S
A
S
L
G
T
R
Q
Site 44
S311
E
P
E
L
D
L
R
S
I
K
E
V
D
E
A
Site 45
T334
K
C
G
E
D
D
E
T
I
P
S
E
Y
R
L
Site 46
S337
E
D
D
E
T
I
P
S
E
Y
R
L
K
P
A
Site 47
Y339
D
E
T
I
P
S
E
Y
R
L
K
P
A
T
D
Site 48
T345
E
Y
R
L
K
P
A
T
D
K
D
G
K
P
L
Site 49
S364
E
E
K
P
K
P
R
S
E
S
E
L
I
D
E
Site 50
S366
K
P
K
P
R
S
E
S
E
L
I
D
E
L
S
Site 51
S373
S
E
L
I
D
E
L
S
E
D
F
D
R
S
E
Site 52
S379
L
S
E
D
F
D
R
S
E
C
K
E
K
P
S
Site 53
S386
S
E
C
K
E
K
P
S
K
P
T
E
K
T
E
Site 54
T392
P
S
K
P
T
E
K
T
E
E
S
K
A
A
A
Site 55
S395
P
T
E
K
T
E
E
S
K
A
A
A
P
A
P
Site 56
T410
V
S
E
A
V
C
R
T
S
M
C
S
I
Q
S
Site 57
S411
S
E
A
V
C
R
T
S
M
C
S
I
Q
S
A
Site 58
S414
V
C
R
T
S
M
C
S
I
Q
S
A
P
P
E
Site 59
S417
T
S
M
C
S
I
Q
S
A
P
P
E
P
A
T
Site 60
T424
S
A
P
P
E
P
A
T
L
K
G
T
V
P
D
Site 61
S440
A
V
E
A
L
A
D
S
L
G
K
K
E
A
D
Site 62
T476
K
L
G
E
K
E
E
T
I
P
P
D
Y
R
L
Site 63
Y481
E
E
T
I
P
P
D
Y
R
L
E
E
V
K
D
Site 64
S499
K
P
L
L
P
K
E
S
K
E
Q
L
P
P
M
Site 65
S507
K
E
Q
L
P
P
M
S
E
D
F
L
L
D
A
Site 66
S516
D
F
L
L
D
A
L
S
E
D
F
S
G
P
Q
Site 67
S520
D
A
L
S
E
D
F
S
G
P
Q
N
A
S
S
Site 68
S527
S
G
P
Q
N
A
S
S
L
K
F
E
D
A
K
Site 69
T546
I
S
E
V
V
S
Q
T
P
A
S
T
T
Q
A
Site 70
S549
V
V
S
Q
T
P
A
S
T
T
Q
A
G
A
P
Site 71
T551
S
Q
T
P
A
S
T
T
Q
A
G
A
P
P
R
Site 72
T560
A
G
A
P
P
R
D
T
S
Q
S
D
K
D
L
Site 73
S561
G
A
P
P
R
D
T
S
Q
S
D
K
D
L
D
Site 74
S563
P
P
R
D
T
S
Q
S
D
K
D
L
D
D
A
Site 75
S575
D
D
A
L
D
K
L
S
D
S
L
G
Q
R
Q
Site 76
S577
A
L
D
K
L
S
D
S
L
G
Q
R
Q
P
D
Site 77
T613
K
L
G
E
R
D
D
T
I
P
P
E
Y
R
H
Site 78
Y618
D
D
T
I
P
P
E
Y
R
H
L
L
D
D
N
Site 79
T635
D
K
P
V
K
P
P
T
K
K
S
E
D
S
K
Site 80
S638
V
K
P
P
T
K
K
S
E
D
S
K
K
P
A
Site 81
S655
Q
D
P
I
D
A
L
S
G
D
L
D
S
C
P
Site 82
S660
A
L
S
G
D
L
D
S
C
P
S
T
T
E
T
Site 83
S663
G
D
L
D
S
C
P
S
T
T
E
T
S
Q
N
Site 84
T664
D
L
D
S
C
P
S
T
T
E
T
S
Q
N
T
Site 85
T667
S
C
P
S
T
T
E
T
S
Q
N
T
A
K
D
Site 86
S681
D
K
C
K
K
A
A
S
S
S
K
A
P
K
N
Site 87
S683
C
K
K
A
A
S
S
S
K
A
P
K
N
G
G
Site 88
S695
N
G
G
K
A
K
D
S
A
K
T
T
E
E
T
Site 89
T698
K
A
K
D
S
A
K
T
T
E
E
T
S
K
P
Site 90
T699
A
K
D
S
A
K
T
T
E
E
T
S
K
P
K
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation