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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
POLR2B
Full Name:
DNA-directed RNA polymerase II subunit RPB2
Alias:
DNA-directed RNA polymerase II subunit B; EC 2.7.7.6; POL2RB; RNA polymerase II subunit B2; RPB2
Type:
Enzyme, DNA-dependent RNA polymerase
Mass (Da):
133897
Number AA:
1174
UniProt ID:
P30876
International Prot ID:
IPI00027808
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0000428
GO:0005634
GO:0005654
Uniprot
OncoNet
Molecular Function:
GO:0000287
GO:0003676
GO:0003677
PhosphoSite+
KinaseNET
Biological Process:
GO:0000375
GO:0000377
GO:0000398
Phosida
TranscriptoNet
STRING
Kinexus Products
DNA-directed RNA polymerase II subunit RPB2 (L848-R859, human) peptide - Powder PE-01ACJ95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ACJ95#DNA-directed RNA polymerase II subunit RPB2 (L848-R859, human) peptide - Powder PE-01AEC95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01AEC95
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y10
D
A
D
E
D
M
Q
Y
D
E
D
D
D
E
I
Site 2
T18
D
E
D
D
D
E
I
T
P
D
L
W
Q
E
A
Site 3
S46
L
V
R
Q
Q
L
D
S
F
D
E
F
I
Q
M
Site 4
Y84
E
V
E
E
P
P
R
Y
L
L
K
F
E
Q
I
Site 5
Y92
L
L
K
F
E
Q
I
Y
L
S
K
P
T
H
W
Site 6
S106
W
E
R
D
G
A
P
S
P
M
M
P
N
E
A
Site 7
T119
E
A
R
L
R
N
L
T
Y
S
A
P
L
Y
V
Site 8
Y120
A
R
L
R
N
L
T
Y
S
A
P
L
Y
V
D
Site 9
S121
R
L
R
N
L
T
Y
S
A
P
L
Y
V
D
I
Site 10
T131
L
Y
V
D
I
T
K
T
V
I
K
E
G
E
E
Site 11
T142
E
G
E
E
Q
L
Q
T
Q
H
Q
K
T
F
I
Site 12
T147
L
Q
T
Q
H
Q
K
T
F
I
G
K
I
P
I
Site 13
Y184
C
P
L
D
P
G
G
Y
F
I
I
N
G
S
E
Site 14
T204
Q
E
K
M
A
T
N
T
V
Y
V
F
A
K
K
Site 15
Y206
K
M
A
T
N
T
V
Y
V
F
A
K
K
D
S
Site 16
S213
Y
V
F
A
K
K
D
S
K
Y
A
Y
T
G
E
Site 17
Y215
F
A
K
K
D
S
K
Y
A
Y
T
G
E
C
R
Site 18
Y217
K
K
D
S
K
Y
A
Y
T
G
E
C
R
S
C
Site 19
T218
K
D
S
K
Y
A
Y
T
G
E
C
R
S
C
L
Site 20
S223
A
Y
T
G
E
C
R
S
C
L
E
N
S
S
R
Site 21
S228
C
R
S
C
L
E
N
S
S
R
P
T
S
T
I
Site 22
S229
R
S
C
L
E
N
S
S
R
P
T
S
T
I
W
Site 23
T232
L
E
N
S
S
R
P
T
S
T
I
W
V
S
M
Site 24
S233
E
N
S
S
R
P
T
S
T
I
W
V
S
M
L
Site 25
T234
N
S
S
R
P
T
S
T
I
W
V
S
M
L
A
Site 26
S238
P
T
S
T
I
W
V
S
M
L
A
R
G
G
Q
Site 27
S250
G
G
Q
G
A
K
K
S
A
I
G
Q
R
I
V
Site 28
S280
F
R
A
L
G
F
V
S
D
R
D
I
L
E
H
Site 29
Y290
D
I
L
E
H
I
I
Y
D
F
E
D
P
E
M
Site 30
Y338
T
K
E
K
R
I
K
Y
A
K
E
V
L
Q
K
Site 31
T359
G
V
S
D
F
C
E
T
K
K
A
Y
F
L
G
Site 32
Y388
E
L
D
D
R
D
H
Y
G
N
K
R
L
D
L
Site 33
Y418
L
L
K
E
V
R
I
Y
A
Q
K
F
I
D
R
Site 34
S447
I
S
D
G
L
K
Y
S
L
A
T
G
N
W
G
Site 35
T474
S
Q
V
L
N
R
L
T
F
A
S
T
L
S
H
Site 36
S477
L
N
R
L
T
F
A
S
T
L
S
H
L
R
R
Site 37
T478
N
R
L
T
F
A
S
T
L
S
H
L
R
R
L
Site 38
S480
L
T
F
A
S
T
L
S
H
L
R
R
L
N
S
Site 39
S487
S
H
L
R
R
L
N
S
P
I
G
R
D
G
K
Site 40
T586
D
P
E
Q
L
M
N
T
L
R
K
L
R
R
Q
Site 41
Y615
R
E
R
E
I
R
I
Y
T
D
A
G
R
I
C
Site 42
T616
E
R
E
I
R
I
Y
T
D
A
G
R
I
C
R
Site 43
Y648
D
Q
L
K
E
R
E
Y
N
N
Y
S
W
Q
D
Site 44
Y651
K
E
R
E
Y
N
N
Y
S
W
Q
D
L
V
A
Site 45
T678
E
T
V
M
L
A
M
T
P
D
D
L
Q
E
K
Site 46
Y689
L
Q
E
K
E
V
A
Y
C
S
T
Y
T
H
C
Site 47
S719
P
F
P
D
H
N
Q
S
P
R
N
T
Y
Q
S
Site 48
T723
H
N
Q
S
P
R
N
T
Y
Q
S
A
M
G
K
Site 49
Y724
N
Q
S
P
R
N
T
Y
Q
S
A
M
G
K
Q
Site 50
S726
S
P
R
N
T
Y
Q
S
A
M
G
K
Q
A
M
Site 51
Y736
G
K
Q
A
M
G
V
Y
I
T
N
F
H
V
R
Site 52
Y752
D
T
L
A
H
V
L
Y
Y
P
Q
K
P
L
V
Site 53
Y753
T
L
A
H
V
L
Y
Y
P
Q
K
P
L
V
T
Site 54
T760
Y
P
Q
K
P
L
V
T
T
R
S
M
E
Y
L
Site 55
S763
K
P
L
V
T
T
R
S
M
E
Y
L
R
F
R
Site 56
Y766
V
T
T
R
S
M
E
Y
L
R
F
R
E
L
P
Site 57
Y788
A
I
A
S
Y
T
G
Y
N
Q
E
D
S
V
I
Site 58
S793
T
G
Y
N
Q
E
D
S
V
I
M
N
R
S
A
Site 59
S808
V
D
R
G
F
F
R
S
V
F
Y
R
S
Y
K
Site 60
S813
F
R
S
V
F
Y
R
S
Y
K
E
Q
E
S
K
Site 61
Y814
R
S
V
F
Y
R
S
Y
K
E
Q
E
S
K
K
Site 62
T836
F
E
K
P
T
R
E
T
C
Q
G
M
R
H
A
Site 63
Y845
Q
G
M
R
H
A
I
Y
D
K
L
D
D
D
G
Site 64
T870
D
D
V
I
I
G
K
T
V
T
L
P
E
N
E
Site 65
T872
V
I
I
G
K
T
V
T
L
P
E
N
E
D
E
Site 66
T883
N
E
D
E
L
E
S
T
N
R
R
Y
T
K
R
Site 67
Y887
L
E
S
T
N
R
R
Y
T
K
R
D
C
S
T
Site 68
T888
E
S
T
N
R
R
Y
T
K
R
D
C
S
T
F
Site 69
S893
R
Y
T
K
R
D
C
S
T
F
L
R
T
S
E
Site 70
T894
Y
T
K
R
D
C
S
T
F
L
R
T
S
E
T
Site 71
S899
C
S
T
F
L
R
T
S
E
T
G
I
V
D
Q
Site 72
T910
I
V
D
Q
V
M
V
T
L
N
Q
E
G
Y
K
Site 73
Y949
K
G
T
C
G
I
Q
Y
R
Q
E
D
M
P
F
Site 74
T957
R
Q
E
D
M
P
F
T
C
E
G
I
T
P
D
Site 75
S990
E
C
L
Q
G
K
V
S
A
N
K
G
E
I
G
Site 76
T1000
K
G
E
I
G
D
A
T
P
F
N
D
A
V
N
Site 77
S1012
A
V
N
V
Q
K
I
S
N
L
L
S
D
Y
G
Site 78
S1016
Q
K
I
S
N
L
L
S
D
Y
G
Y
H
L
R
Site 79
Y1018
I
S
N
L
L
S
D
Y
G
Y
H
L
R
G
N
Site 80
Y1020
N
L
L
S
D
Y
G
Y
H
L
R
G
N
E
V
Site 81
Y1029
L
R
G
N
E
V
L
Y
N
G
F
T
G
R
K
Site 82
T1033
E
V
L
Y
N
G
F
T
G
R
K
I
T
S
Q
Site 83
T1046
S
Q
I
F
I
G
P
T
Y
Y
Q
R
L
K
H
Site 84
Y1047
Q
I
F
I
G
P
T
Y
Y
Q
R
L
K
H
M
Site 85
S1111
R
E
R
L
F
E
A
S
D
P
Y
Q
V
H
V
Site 86
T1134
I
A
N
T
R
T
H
T
Y
E
C
R
G
C
R
Site 87
Y1135
A
N
T
R
T
H
T
Y
E
C
R
G
C
R
N
Site 88
T1144
C
R
G
C
R
N
K
T
Q
I
S
L
V
R
M
Site 89
S1147
C
R
N
K
T
Q
I
S
L
V
R
M
P
Y
A
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation