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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
GTF2F1
Full Name:
General transcription factor IIF subunit 1
Alias:
BTF4; General transcription factor IIF polypeptide 1 74 kDa subunit protein; General transcription factor IIF, polypeptide 1 (74kD subunit); General transcription factor IIF, polypeptide 1, 74kDa; RAP74; T2FA; TF2F1; TFIIF; Transcription initiation factor IIF alpha subunit; Transcription initiation factor IIF subunit alpha; Transcription initiation factor RAP74
Type:
EC 2.7.11.1; Transcription initiation complex
Mass (Da):
58240
Number AA:
517
UniProt ID:
P35269
International Prot ID:
IPI00017450
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005674
Uniprot
OncoNet
Molecular Function:
GO:0003677
GO:0003824
GO:0016251
PhosphoSite+
KinaseNET
Biological Process:
GO:0006368
GO:0000398
GO:0045941
Phosida
TranscriptoNet
STRING
Kinexus Products
General transcription factor IIF subunit 1 S385+T389 phosphosite-specific antibody AB-PK651#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK651#General transcription factor IIF subunit 1 (R382-A392, human) pS385+pT389 phosphopeptide - Powder PE-04AMS95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AMS95
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S8
M
A
A
L
G
P
S
S
Q
N
V
T
E
Y
V
Site 2
T12
G
P
S
S
Q
N
V
T
E
Y
V
V
R
V
P
Site 3
Y14
S
S
Q
N
V
T
E
Y
V
V
R
V
P
K
N
Site 4
T22
V
V
R
V
P
K
N
T
T
K
K
Y
N
I
M
Site 5
Y26
P
K
N
T
T
K
K
Y
N
I
M
A
F
N
A
Site 6
S52
A
R
L
E
R
D
L
S
N
K
K
I
Y
Q
E
Site 7
S65
Q
E
E
E
M
P
E
S
G
A
G
S
E
F
N
Site 8
S69
M
P
E
S
G
A
G
S
E
F
N
R
K
L
R
Site 9
Y84
E
E
A
R
R
K
K
Y
G
I
V
L
K
E
F
Site 10
S106
L
L
R
V
N
G
K
S
G
R
K
F
K
G
I
Site 11
T119
G
I
K
K
G
G
V
T
E
N
T
S
Y
Y
I
Site 12
Y124
G
V
T
E
N
T
S
Y
Y
I
F
T
Q
C
P
Site 13
Y125
V
T
E
N
T
S
Y
Y
I
F
T
Q
C
P
D
Site 14
Y144
A
F
P
V
H
N
W
Y
N
F
T
P
L
A
R
Site 15
T154
T
P
L
A
R
H
R
T
L
T
A
E
E
A
E
Site 16
T156
L
A
R
H
R
T
L
T
A
E
E
A
E
E
E
Site 17
S203
K
R
G
R
R
K
A
S
E
L
R
I
H
D
L
Site 18
S217
L
E
D
D
L
E
M
S
S
D
A
S
D
A
S
Site 19
S218
E
D
D
L
E
M
S
S
D
A
S
D
A
S
G
Site 20
S221
L
E
M
S
S
D
A
S
D
A
S
G
E
E
G
Site 21
S224
S
S
D
A
S
D
A
S
G
E
E
G
G
R
V
Site 22
S253
K
K
K
K
K
K
G
S
D
D
E
A
F
E
D
Site 23
S261
D
D
E
A
F
E
D
S
D
D
G
D
F
E
G
Site 24
Y273
F
E
G
Q
E
V
D
Y
M
S
D
G
S
S
S
Site 25
S275
G
Q
E
V
D
Y
M
S
D
G
S
S
S
S
Q
Site 26
S278
V
D
Y
M
S
D
G
S
S
S
S
Q
E
E
P
Site 27
S279
D
Y
M
S
D
G
S
S
S
S
Q
E
E
P
E
Site 28
S280
Y
M
S
D
G
S
S
S
S
Q
E
E
P
E
S
Site 29
S281
M
S
D
G
S
S
S
S
Q
E
E
P
E
S
K
Site 30
S287
S
S
Q
E
E
P
E
S
K
A
K
A
P
Q
Q
Site 31
S305
P
K
G
V
D
E
Q
S
D
S
S
E
E
S
E
Site 32
S307
G
V
D
E
Q
S
D
S
S
E
E
S
E
E
E
Site 33
S308
V
D
E
Q
S
D
S
S
E
E
S
E
E
E
K
Site 34
S311
Q
S
D
S
S
E
E
S
E
E
E
K
P
P
E
Site 35
T331
E
E
E
K
K
A
P
T
P
Q
E
K
K
R
R
Site 36
S341
E
K
K
R
R
K
D
S
S
E
E
S
D
S
S
Site 37
S342
K
K
R
R
K
D
S
S
E
E
S
D
S
S
E
Site 38
S345
R
K
D
S
S
E
E
S
D
S
S
E
E
S
D
Site 39
S347
D
S
S
E
E
S
D
S
S
E
E
S
D
I
D
Site 40
S348
S
S
E
E
S
D
S
S
E
E
S
D
I
D
S
Site 41
S351
E
S
D
S
S
E
E
S
D
I
D
S
E
A
S
Site 42
S355
S
E
E
S
D
I
D
S
E
A
S
S
A
L
F
Site 43
S358
S
D
I
D
S
E
A
S
S
A
L
F
M
A
K
Site 44
S359
D
I
D
S
E
A
S
S
A
L
F
M
A
K
K
Site 45
T368
L
F
M
A
K
K
K
T
P
P
K
R
E
R
K
Site 46
S377
P
K
R
E
R
K
P
S
G
G
S
S
R
G
N
Site 47
S380
E
R
K
P
S
G
G
S
S
R
G
N
S
R
P
Site 48
S381
R
K
P
S
G
G
S
S
R
G
N
S
R
P
G
Site 49
S385
G
G
S
S
R
G
N
S
R
P
G
T
P
S
A
General transcription factor IIF subunit 1 S385+T389 phosphosite-specific antibody AB-PK651#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK651#General transcription factor IIF subunit 1 (R382-A392, human) pS385+pT389 phosphopeptide - Powder PE-04AMS95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AMS95
Site 50
T389
R
G
N
S
R
P
G
T
P
S
A
E
G
G
S
General transcription factor IIF subunit 1 S385+T389 phosphosite-specific antibody AB-PK651#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK651#General transcription factor IIF subunit 1 (R382-A392, human) pS385+pT389 phosphopeptide - Powder PE-04AMS95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AMS95
Site 51
S391
N
S
R
P
G
T
P
S
A
E
G
G
S
T
S
Site 52
S396
T
P
S
A
E
G
G
S
T
S
S
T
L
R
A
Site 53
T397
P
S
A
E
G
G
S
T
S
S
T
L
R
A
A
Site 54
S399
A
E
G
G
S
T
S
S
T
L
R
A
A
A
S
Site 55
T400
E
G
G
S
T
S
S
T
L
R
A
A
A
S
K
Site 56
S415
L
E
Q
G
K
R
V
S
E
M
P
A
A
K
R
Site 57
T427
A
K
R
L
R
L
D
T
G
P
Q
S
L
S
G
Site 58
S431
R
L
D
T
G
P
Q
S
L
S
G
K
S
T
P
Site 59
S433
D
T
G
P
Q
S
L
S
G
K
S
T
P
Q
P
Site 60
S436
P
Q
S
L
S
G
K
S
T
P
Q
P
P
S
G
Site 61
T437
Q
S
L
S
G
K
S
T
P
Q
P
P
S
G
K
Site 62
S442
K
S
T
P
Q
P
P
S
G
K
T
T
P
N
S
Site 63
T445
P
Q
P
P
S
G
K
T
T
P
N
S
G
D
V
Site 64
T446
Q
P
P
S
G
K
T
T
P
N
S
G
D
V
Q
Site 65
S449
S
G
K
T
T
P
N
S
G
D
V
Q
V
T
E
Site 66
Y462
T
E
D
A
V
R
R
Y
L
T
R
K
P
M
T
Site 67
T464
D
A
V
R
R
Y
L
T
R
K
P
M
T
T
K
Site 68
T470
L
T
R
K
P
M
T
T
K
D
L
L
K
K
F
Site 69
T479
D
L
L
K
K
F
Q
T
K
K
T
G
L
S
S
Site 70
T482
K
K
F
Q
T
K
K
T
G
L
S
S
E
Q
T
Site 71
T489
T
G
L
S
S
E
Q
T
V
N
V
L
A
Q
I
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation