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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
USP8
Full Name:
Ubiquitin carboxyl-terminal hydrolase 8
Alias:
Deubiquitinating enzyme 8; Ubiquitin thioesterase 8; Ubiquitin-specific-processing protease 8
Type:
Protease; Ubiquitin conjugating system; EC 3.1.2.15
Mass (Da):
127523
Number AA:
1118
UniProt ID:
P40818
International Prot ID:
IPI00030915
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005622
Uniprot
OncoNet
Molecular Function:
GO:0017124
GO:0004197
GO:0004221
PhosphoSite+
KinaseNET
Biological Process:
GO:0008283
GO:0006511
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y12
A
S
V
P
K
E
L
Y
L
S
S
S
L
K
D
Site 2
S14
V
P
K
E
L
Y
L
S
S
S
L
K
D
L
N
Site 3
S16
K
E
L
Y
L
S
S
S
L
K
D
L
N
K
K
Site 4
S32
E
V
K
P
E
K
I
S
T
K
S
Y
V
H
S
Site 5
T33
V
K
P
E
K
I
S
T
K
S
Y
V
H
S
A
Site 6
S35
P
E
K
I
S
T
K
S
Y
V
H
S
A
L
K
Site 7
Y36
E
K
I
S
T
K
S
Y
V
H
S
A
L
K
I
Site 8
S39
S
T
K
S
Y
V
H
S
A
L
K
I
F
K
T
Site 9
T46
S
A
L
K
I
F
K
T
A
E
E
C
R
L
D
Site 10
Y60
D
R
D
E
E
R
A
Y
V
L
Y
M
K
Y
V
Site 11
Y63
E
E
R
A
Y
V
L
Y
M
K
Y
V
T
V
Y
Site 12
Y85
D
F
K
Q
Q
Q
D
Y
F
H
S
I
L
G
P
Site 13
S106
V
E
E
A
E
R
L
S
E
S
L
K
L
R
Y
Site 14
S108
E
A
E
R
L
S
E
S
L
K
L
R
Y
E
E
Site 15
T141
L
Q
Q
K
R
Q
E
T
G
R
E
D
G
G
T
Site 16
T148
T
G
R
E
D
G
G
T
L
A
K
G
S
L
E
Site 17
S153
G
G
T
L
A
K
G
S
L
E
N
V
L
D
S
Site 18
S160
S
L
E
N
V
L
D
S
K
D
K
T
Q
K
S
Site 19
T164
V
L
D
S
K
D
K
T
Q
K
S
N
G
E
K
Site 20
S167
S
K
D
K
T
Q
K
S
N
G
E
K
N
E
K
Site 21
T177
E
K
N
E
K
C
E
T
K
E
K
G
A
I
T
Site 22
T193
K
E
L
Y
T
M
M
T
D
K
N
I
S
L
I
Site 23
Y210
D
A
R
R
M
Q
D
Y
Q
D
S
C
I
L
H
Site 24
S213
R
M
Q
D
Y
Q
D
S
C
I
L
H
S
L
S
Site 25
S218
Q
D
S
C
I
L
H
S
L
S
V
P
E
E
A
Site 26
S227
S
V
P
E
E
A
I
S
P
G
V
T
A
S
W
Site 27
S243
E
A
H
L
P
D
D
S
K
D
T
W
K
K
R
Site 28
T246
L
P
D
D
S
K
D
T
W
K
K
R
G
N
V
Site 29
Y255
K
K
R
G
N
V
E
Y
V
V
L
L
D
W
F
Site 30
T272
A
K
D
L
Q
I
G
T
T
L
R
S
L
K
D
Site 31
T273
K
D
L
Q
I
G
T
T
L
R
S
L
K
D
A
Site 32
S276
Q
I
G
T
T
L
R
S
L
K
D
A
L
F
K
Site 33
S286
D
A
L
F
K
W
E
S
K
T
V
L
R
N
E
Site 34
T288
L
F
K
W
E
S
K
T
V
L
R
N
E
P
L
Site 35
Y311
W
L
L
C
Y
P
Q
Y
T
T
N
A
K
V
T
Site 36
T318
Y
T
T
N
A
K
V
T
P
P
P
R
R
Q
N
Site 37
S331
Q
N
E
E
V
S
I
S
L
D
F
T
Y
P
S
Site 38
T335
V
S
I
S
L
D
F
T
Y
P
S
L
E
E
S
Site 39
Y336
S
I
S
L
D
F
T
Y
P
S
L
E
E
S
I
Site 40
S338
S
L
D
F
T
Y
P
S
L
E
E
S
I
P
S
Site 41
S342
T
Y
P
S
L
E
E
S
I
P
S
K
P
A
A
Site 42
S345
S
L
E
E
S
I
P
S
K
P
A
A
Q
T
P
Site 43
T351
P
S
K
P
A
A
Q
T
P
P
A
S
I
E
V
Site 44
S355
A
A
Q
T
P
P
A
S
I
E
V
D
E
N
I
Site 45
S378
R
M
G
P
L
N
I
S
T
P
V
E
P
V
A
Site 46
T379
M
G
P
L
N
I
S
T
P
V
E
P
V
A
A
Site 47
S387
P
V
E
P
V
A
A
S
K
S
D
V
S
P
I
Site 48
S389
E
P
V
A
A
S
K
S
D
V
S
P
I
I
Q
Site 49
S392
A
A
S
K
S
D
V
S
P
I
I
Q
P
V
P
Site 50
S400
P
I
I
Q
P
V
P
S
I
K
N
V
P
Q
I
Site 51
T410
N
V
P
Q
I
D
R
T
K
K
P
A
V
K
L
Site 52
S425
P
E
E
H
R
I
K
S
E
S
T
N
H
E
Q
Site 53
S427
E
H
R
I
K
S
E
S
T
N
H
E
Q
Q
S
Site 54
S434
S
T
N
H
E
Q
Q
S
P
Q
S
G
K
V
I
Site 55
S437
H
E
Q
Q
S
P
Q
S
G
K
V
I
P
D
R
Site 56
S445
G
K
V
I
P
D
R
S
T
K
P
V
V
F
S
Site 57
T446
K
V
I
P
D
R
S
T
K
P
V
V
F
S
P
Site 58
S452
S
T
K
P
V
V
F
S
P
T
L
M
L
T
D
Site 59
T454
K
P
V
V
F
S
P
T
L
M
L
T
D
E
E
Site 60
T515
E
A
E
E
N
E
I
T
E
K
Q
Q
K
A
K
Site 61
S530
E
E
M
E
K
K
E
S
E
Q
A
K
K
E
D
Site 62
T540
A
K
K
E
D
K
E
T
S
A
K
R
G
K
E
Site 63
S541
K
K
E
D
K
E
T
S
A
K
R
G
K
E
I
Site 64
T549
A
K
R
G
K
E
I
T
G
V
K
R
Q
S
K
Site 65
S555
I
T
G
V
K
R
Q
S
K
S
E
H
E
T
S
Site 66
S557
G
V
K
R
Q
S
K
S
E
H
E
T
S
D
A
Site 67
T561
Q
S
K
S
E
H
E
T
S
D
A
K
K
S
V
Site 68
S562
S
K
S
E
H
E
T
S
D
A
K
K
S
V
E
Site 69
S567
E
T
S
D
A
K
K
S
V
E
D
R
G
K
R
Site 70
T577
D
R
G
K
R
C
P
T
P
E
I
Q
K
K
S
Site 71
S584
T
P
E
I
Q
K
K
S
T
G
D
V
P
H
T
Site 72
T591
S
T
G
D
V
P
H
T
S
V
T
G
D
S
G
Site 73
S592
T
G
D
V
P
H
T
S
V
T
G
D
S
G
S
Site 74
T594
D
V
P
H
T
S
V
T
G
D
S
G
S
G
K
Site 75
S597
H
T
S
V
T
G
D
S
G
S
G
K
P
F
K
Site 76
S599
S
V
T
G
D
S
G
S
G
K
P
F
K
I
K
Site 77
S611
K
I
K
G
Q
P
E
S
G
I
L
R
T
G
T
Site 78
T618
S
G
I
L
R
T
G
T
F
R
E
D
T
D
D
Site 79
T623
T
G
T
F
R
E
D
T
D
D
T
E
R
N
K
Site 80
T626
F
R
E
D
T
D
D
T
E
R
N
K
A
Q
R
Site 81
T637
K
A
Q
R
E
P
L
T
R
A
R
S
E
E
M
Site 82
S641
E
P
L
T
R
A
R
S
E
E
M
G
R
I
V
Site 83
S653
R
I
V
P
G
L
P
S
G
W
A
K
F
L
D
Site 84
T663
A
K
F
L
D
P
I
T
G
T
F
R
Y
Y
H
Site 85
T665
F
L
D
P
I
T
G
T
F
R
Y
Y
H
S
P
Site 86
Y668
P
I
T
G
T
F
R
Y
Y
H
S
P
T
N
T
Site 87
Y669
I
T
G
T
F
R
Y
Y
H
S
P
T
N
T
V
Site 88
S671
G
T
F
R
Y
Y
H
S
P
T
N
T
V
H
M
Site 89
T673
F
R
Y
Y
H
S
P
T
N
T
V
H
M
Y
P
Site 90
T675
Y
Y
H
S
P
T
N
T
V
H
M
Y
P
P
E
Site 91
Y679
P
T
N
T
V
H
M
Y
P
P
E
M
A
P
S
Site 92
S686
Y
P
P
E
M
A
P
S
S
A
P
P
S
T
P
Site 93
S687
P
P
E
M
A
P
S
S
A
P
P
S
T
P
P
Site 94
S691
A
P
S
S
A
P
P
S
T
P
P
T
H
K
A
Site 95
T692
P
S
S
A
P
P
S
T
P
P
T
H
K
A
K
Site 96
T695
A
P
P
S
T
P
P
T
H
K
A
K
P
Q
I
Site 97
S711
A
E
R
D
R
E
P
S
K
L
K
R
S
Y
S
Site 98
S716
E
P
S
K
L
K
R
S
Y
S
S
P
D
I
T
Site 99
Y717
P
S
K
L
K
R
S
Y
S
S
P
D
I
T
Q
Site 100
S718
S
K
L
K
R
S
Y
S
S
P
D
I
T
Q
A
Site 101
S719
K
L
K
R
S
Y
S
S
P
D
I
T
Q
A
I
Site 102
T723
S
Y
S
S
P
D
I
T
Q
A
I
Q
E
E
E
Site 103
T735
E
E
E
K
R
K
P
T
V
T
P
T
V
N
R
Site 104
T737
E
K
R
K
P
T
V
T
P
T
V
N
R
E
N
Site 105
T739
R
K
P
T
V
T
P
T
V
N
R
E
N
K
P
Site 106
T747
V
N
R
E
N
K
P
T
C
Y
P
K
A
E
I
Site 107
Y749
R
E
N
K
P
T
C
Y
P
K
A
E
I
S
R
Site 108
S755
C
Y
P
K
A
E
I
S
R
L
S
A
S
Q
I
Site 109
S758
K
A
E
I
S
R
L
S
A
S
Q
I
R
N
L
Site 110
S760
E
I
S
R
L
S
A
S
Q
I
R
N
L
N
P
Site 111
T777
G
G
S
G
P
A
L
T
G
L
R
N
L
G
N
Site 112
Y787
R
N
L
G
N
T
C
Y
M
N
S
I
L
Q
C
Site 113
Y804
N
A
P
H
L
A
D
Y
F
N
R
N
C
Y
Q
Site 114
Y810
D
Y
F
N
R
N
C
Y
Q
D
D
I
N
R
S
Site 115
Y842
K
A
L
W
T
G
Q
Y
R
Y
I
S
P
K
D
Site 116
Y844
L
W
T
G
Q
Y
R
Y
I
S
P
K
D
F
K
Site 117
S846
T
G
Q
Y
R
Y
I
S
P
K
D
F
K
I
T
Site 118
S865
N
D
Q
F
A
G
Y
S
Q
Q
D
S
Q
E
L
Site 119
S869
A
G
Y
S
Q
Q
D
S
Q
E
L
L
L
F
L
Site 120
Y893
K
A
D
N
R
K
R
Y
K
E
E
N
N
D
H
Site 121
T938
K
S
T
V
Q
C
L
T
C
H
K
K
S
R
T
Site 122
T945
T
C
H
K
K
S
R
T
F
E
A
F
M
Y
L
Site 123
Y951
R
T
F
E
A
F
M
Y
L
S
L
P
L
A
S
Site 124
S960
S
L
P
L
A
S
T
S
K
C
T
L
Q
D
C
Site 125
S972
Q
D
C
L
R
L
F
S
K
E
E
K
L
T
D
Site 126
T978
F
S
K
E
E
K
L
T
D
N
N
R
F
Y
C
Site 127
Y984
L
T
D
N
N
R
F
Y
C
S
H
C
R
A
R
Site 128
S994
H
C
R
A
R
R
D
S
L
K
K
I
E
I
W
Site 129
S1015
L
V
H
L
K
R
F
S
Y
D
G
R
W
K
Q
Site 130
Y1016
V
H
L
K
R
F
S
Y
D
G
R
W
K
Q
K
Site 131
T1026
R
W
K
Q
K
L
Q
T
S
V
D
F
P
L
E
Site 132
S1027
W
K
Q
K
L
Q
T
S
V
D
F
P
L
E
N
Site 133
S1038
P
L
E
N
L
D
L
S
Q
Y
V
I
G
P
K
Site 134
Y1040
E
N
L
D
L
S
Q
Y
V
I
G
P
K
N
N
Site 135
Y1051
P
K
N
N
L
K
K
Y
N
L
F
S
V
S
N
Site 136
S1055
L
K
K
Y
N
L
F
S
V
S
N
H
Y
G
G
Site 137
S1057
K
Y
N
L
F
S
V
S
N
H
Y
G
G
L
D
Site 138
Y1068
G
G
L
D
G
G
H
Y
T
A
Y
C
K
N
A
Site 139
Y1071
D
G
G
H
Y
T
A
Y
C
K
N
A
A
R
Q
Site 140
S1089
K
F
D
D
H
E
V
S
D
I
S
V
S
S
V
Site 141
S1092
D
H
E
V
S
D
I
S
V
S
S
V
K
S
S
Site 142
S1094
E
V
S
D
I
S
V
S
S
V
K
S
S
A
A
Site 143
T1114
T
S
L
G
P
R
V
T
D
V
A
T
_
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation