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Protein Info
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Short Name:
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Cot
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Full Name:
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Mitogen-activated protein kinase kinase kinase 8
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Alias:
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EC 2.7.11.25; M3K8; MAP3K8; Tpl2; TPL2; TPL-2; Tumor progression locus 2
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Type:
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Protein-serine kinase, STE group, STE-Unique family
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Mass (Da):
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52925
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Number AA:
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467
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UniProt ID:
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P41279
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International Prot ID:
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IPI00759772
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Sequence:
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Retrieve full protein sequence
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GO Terms
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External Links
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Internal Links
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Cellular Component:
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GO:0005829
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Uniprot
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OncoNet
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Molecular Function:
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GO:0005524
GO:0004709
GO:0000287
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PhosphoSite+
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KinaseNET
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Biological Process:
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GO:0007049
GO:0006468
GO:0007165
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Phosida
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TranscriptoNet
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STRING
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Kinexus Products
Osaka thyroid oncogene protein-serine kinase (Tpl2); Mitogen-activated protein kinase kinase kinase 8 pan-specific antibody AB-NK042#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK042#Osaka thyroid oncogene protein-serine kinase (Tpl2); Mitogen-activated protein kinase kinase kinase 8 pan-specific antibody AB-NK042-1#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK042-1#Osaka thyroid oncogene protein-serine kinase (Tpl2); Mitogen-activated protein kinase kinase kinase 8 pan-specific antibody AB-NK042-2#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK042-2#Osaka thyroid oncogene protein-serine kinase (Tpl2); Mitogen-activated protein kinase kinase kinase 8 S334 phosphosite-specific antibody AB-PK585#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK585#Osaka thyroid oncogene protein-serine kinase (Tpl2) / Mitogen-activated protein kinase kinase kinase 8 (Y331-P337, human) pS334 phosphopeptide - Powder PE-04AKB90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AKB90
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Info Box
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A variety of parameters were considered in the selection of putative P-sites.
Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score
is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr
or P-Tyr sites. The Maximum KInase Score provides the calculated score for
highest match of 500 human protein kinases for the amino acid sequence
surrounding the target P-site as determined with Kinase Substrate Predictor V2.
The Sum KInase Score provides the additive sum of the positive individual Kinase
Substrate Predictor V2 scores from 500 human protein kinases. The Conservation
Score is the average of the percent similarity of the human P-site with the
equivalent P-site in 20 other diverse species. Click the coloured buttons below
to retrieve this and other information in the Info Box, or click the orange
buttons for relevant links. |
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| Phosphosites | |
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| | | -7 | -6 | -5 | -4 | -3 | -2 | -1 | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | | Expt. conf. | Effect | Kinase | PPase | Kinexus Products | | Ref. | Evol. | Kinase Pred. | P-site Match |
| | Site 1 | Y34 | I | D | I | M | E | N | L | Y | A | S | E | E | P | A | V | |  |  | | | | | |  | | |
| | Site 2 | Y42 | A | S | E | E | P | A | V | Y | E | P | S | L | M | T | M | | | | | | | | | | | |
| | Site 3 | S60 | S | N | Q | N | D | E | R | S | K | S | L | L | L | S | G | | | | | | | | | | | |
| | Site 4 | S62 | Q | N | D | E | R | S | K | S | L | L | L | S | G | Q | E | |  | | | | | | | | | |
| | Site 5 | S66 | R | S | K | S | L | L | L | S | G | Q | E | V | P | W | L | | | | | | | | | | | |
| | Site 6 | S74 | G | Q | E | V | P | W | L | S | S | V | R | Y | G | T | V | | | | | | | | | | | |
| | Site 7 | S75 | Q | E | V | P | W | L | S | S | V | R | Y | G | T | V | E | | | | | | | | | | | |
| | Site 8 | Y78 | P | W | L | S | S | V | R | Y | G | T | V | E | D | L | L | | | | | | | | | | | |
| | Site 9 | T80 | L | S | S | V | R | Y | G | T | V | E | D | L | L | A | F | | | | | | | | | | | |
| | Site 10 | T115 | I | L | L | N | M | V | I | T | P | Q | N | G | R | Y | Q | | | | | | | | | | | |
| | Site 11 | Y121 | I | T | P | Q | N | G | R | Y | Q | I | D | S | D | V | L | | | | | | | | | | | |
| | Site 12 | S125 | N | G | R | Y | Q | I | D | S | D | V | L | L | I | P | W | | | | | | | | | | | |
| | Site 13 | T135 | L | L | I | P | W | K | L | T | Y | R | N | I | G | S | D | | | | | | | | | | | |
| | Site 14 | Y136 | L | I | P | W | K | L | T | Y | R | N | I | G | S | D | F | | | | | | | | | | | |
| | Site 15 | S141 | L | T | Y | R | N | I | G | S | D | F | I | P | R | G | A | | | | | | | | | | | |
| | Site 16 | Y153 | R | G | A | F | G | K | V | Y | L | A | Q | D | I | K | T | | | | | | | | | | | |
| | Site 17 | T160 | Y | L | A | Q | D | I | K | T | K | K | R | M | A | C | K | | | | | | | | | | | |
| | Site 18 | S177 | P | V | D | Q | F | K | P | S | D | V | E | I | Q | A | C | | | | | | | | | | | |
| | Site 19 | Y194 | H | E | N | I | A | E | L | Y | G | A | V | L | W | G | E | | | | | | | | | | | |
| | Site 20 | S214 | M | E | A | G | E | G | G | S | V | L | E | K | L | E | S | | | | | | | | | | | |
| | Site 21 | S221 | S | V | L | E | K | L | E | S | C | G | P | M | R | E | F | | | | | | | | | | | |
| | Site 22 | S246 | K | G | L | D | F | L | H | S | K | K | V | I | H | H | D | | | | | | | | | | | |
| | Site 23 | T278 | F | G | L | S | V | Q | M | T | E | D | V | Y | F | P | K | | | | | | | | | | | |
| | Site 24 | Y282 | V | Q | M | T | E | D | V | Y | F | P | K | D | L | R | G | | | | | | | | | | | |
| | Site 25 | T290 | F | P | K | D | L | R | G | T | E | I | Y | M | S | P | E | | | | | | | | | | | |
| | Site 26 | S295 | R | G | T | E | I | Y | M | S | P | E | V | I | L | C | R | | | | | | | | | | | |
| | Site 27 | T322 | A | T | L | I | H | M | Q | T | G | T | P | P | W | V | K | | | | | | | | | | | |
| | Site 28 | T324 | L | I | H | M | Q | T | G | T | P | P | W | V | K | R | Y | | | | | | | | | | | |
| | Site 29 | Y331 | T | P | P | W | V | K | R | Y | P | R | S | A | Y | P | S | | | | | | | | | | | |
| | Site 30 | S334 | W | V | K | R | Y | P | R | S | A | Y | P | S | Y | L | Y | | | | | |  Osaka thyroid oncogene protein-serine kinase (Tpl2); Mitogen-activated protein kinase kinase kinase 8 S334 phosphosite-specific antibody AB-PK585#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK585#Osaka thyroid oncogene protein-serine kinase (Tpl2) / Mitogen-activated protein kinase kinase kinase 8 (Y331-P337, human) pS334 phosphopeptide - Powder PE-04AKB90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AKB90 | | | | | |
| | Site 31 | Y336 | K | R | Y | P | R | S | A | Y | P | S | Y | L | Y | I | I | | | | | | | | | | | |
| | Site 32 | S338 | Y | P | R | S | A | Y | P | S | Y | L | Y | I | I | H | K | | | | | | | | | | | |
| | Site 33 | Y339 | P | R | S | A | Y | P | S | Y | L | Y | I | I | H | K | Q | | | | | | | | | | | |
| | Site 34 | Y341 | S | A | Y | P | S | Y | L | Y | I | I | H | K | Q | A | P | | | | | | | | | | | |
| | Site 35 | S358 | E | D | I | A | D | D | C | S | P | G | M | R | E | L | I | | | | | | | | | | | |
| | Site 36 | S368 | M | R | E | L | I | E | A | S | L | E | R | N | P | N | H | | | | | | | | | | | |
| | Site 37 | S400 | E | D | Q | P | R | C | Q | S | L | D | S | A | L | L | E | | | | | | | | | | | |
| | Site 38 | S413 | L | E | R | K | R | L | L | S | R | K | E | L | E | L | P | | | | | | | | | | | |
| | Site 39 | S426 | L | P | E | N | I | A | D | S | S | C | T | G | S | T | E | | | | | | | | | | | |
| | Site 40 | S427 | P | E | N | I | A | D | S | S | C | T | G | S | T | E | E | | | | | | | | | | | |
| | Site 41 | S431 | A | D | S | S | C | T | G | S | T | E | E | S | E | M | L | | | | | | | | | | | |
| | Site 42 | S435 | C | T | G | S | T | E | E | S | E | M | L | K | R | Q | R | | | | | | | | | | | |
| | Site 43 | S443 | E | M | L | K | R | Q | R | S | L | Y | I | D | L | G | A | | | | | | | | | | | |
| | Site 44 | Y445 | L | K | R | Q | R | S | L | Y | I | D | L | G | A | L | A | | | | | | | | | | | |
| | Site 45 | T463 | N | L | V | R | G | P | P | T | L | E | Y | G | _ | _ | _ | | | | | | | | | | | |
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Legend
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Confirmed in mammals
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Confirmed in related proteins or other species
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Predicted by Kinexus P-Site Prediction algorithm
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No data/link available
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Link available
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Products available
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