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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
NEK4
Full Name:
Serine/threonine-protein kinase Nek4
Alias:
EC 2.7.11.1; Kinase Nek4; NIMA (never in mitosis gene a)-related kinase 4; NimA-related protein kinase 4; NRK2; Pp12301; Serine/threonine protein kinase 2; Serine/threonine protein kinase-2; STK2
Type:
Protein kinase, tyrosine (non-receptor); EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family
Mass (Da):
94597
Number AA:
841
UniProt ID:
P51957
International Prot ID:
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005634
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0000287
GO:0004674
PhosphoSite+
KinaseNET
Biological Process:
GO:0051301
GO:0007067
GO:0006468
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y17
R
V
V
G
K
G
S
Y
G
E
V
T
L
V
K
Site 2
T21
K
G
S
Y
G
E
V
T
L
V
K
H
R
R
D
Site 3
Y32
H
R
R
D
G
K
Q
Y
V
I
K
K
L
N
L
Site 4
S43
K
L
N
L
R
N
A
S
S
R
E
R
R
A
A
Site 5
S44
L
N
L
R
N
A
S
S
R
E
R
R
A
A
E
Site 6
S58
E
Q
E
A
Q
L
L
S
Q
L
K
H
P
N
I
Site 7
Y68
K
H
P
N
I
V
T
Y
K
E
S
W
E
G
G
Site 8
S71
N
I
V
T
Y
K
E
S
W
E
G
G
D
G
L
Site 9
Y92
F
C
E
G
G
D
L
Y
R
K
L
K
E
Q
K
Site 10
T134
I
L
H
R
D
L
K
T
Q
N
V
F
L
T
R
Site 11
T169
M
A
S
T
L
I
G
T
P
Y
Y
M
S
P
E
Site 12
S174
I
G
T
P
Y
Y
M
S
P
E
L
F
S
N
K
Site 13
Y183
E
L
F
S
N
K
P
Y
N
Y
K
S
D
V
W
Site 14
Y216
K
D
M
N
S
L
V
Y
R
I
I
E
G
K
L
Site 15
Y230
L
P
P
M
P
R
D
Y
S
P
E
L
A
E
L
Site 16
S231
P
P
M
P
R
D
Y
S
P
E
L
A
E
L
I
Site 17
T240
E
L
A
E
L
I
R
T
M
L
S
K
R
P
E
Site 18
S243
E
L
I
R
T
M
L
S
K
R
P
E
E
R
P
Site 19
S251
K
R
P
E
E
R
P
S
V
R
S
I
L
R
Q
Site 20
S254
E
E
R
P
S
V
R
S
I
L
R
Q
P
Y
I
Site 21
Y260
R
S
I
L
R
Q
P
Y
I
K
R
Q
I
S
F
Site 22
S266
P
Y
I
K
R
Q
I
S
F
F
L
E
A
T
K
Site 23
S286
N
N
I
K
N
G
D
S
Q
S
K
P
F
A
T
Site 24
S288
I
K
N
G
D
S
Q
S
K
P
F
A
T
V
V
Site 25
T293
S
Q
S
K
P
F
A
T
V
V
S
G
E
A
E
Site 26
S296
K
P
F
A
T
V
V
S
G
E
A
E
S
N
H
Site 27
S312
V
I
H
P
Q
P
L
S
S
E
G
S
Q
T
Y
Site 28
S313
I
H
P
Q
P
L
S
S
E
G
S
Q
T
Y
I
Site 29
S316
Q
P
L
S
S
E
G
S
Q
T
Y
I
M
G
E
Site 30
T318
L
S
S
E
G
S
Q
T
Y
I
M
G
E
G
K
Site 31
Y319
S
S
E
G
S
Q
T
Y
I
M
G
E
G
K
C
Site 32
S328
M
G
E
G
K
C
L
S
Q
E
K
P
R
A
S
Site 33
S335
S
Q
E
K
P
R
A
S
G
L
L
K
S
P
A
Site 34
S340
R
A
S
G
L
L
K
S
P
A
S
L
K
A
H
Site 35
S343
G
L
L
K
S
P
A
S
L
K
A
H
T
C
K
Site 36
S354
H
T
C
K
Q
D
L
S
N
T
T
E
L
A
T
Site 37
S377
L
P
A
K
G
R
D
S
V
S
D
G
F
V
Q
Site 38
S379
A
K
G
R
D
S
V
S
D
G
F
V
Q
E
N
Site 39
Y390
V
Q
E
N
Q
P
R
Y
L
D
A
S
N
E
L
Site 40
S394
Q
P
R
Y
L
D
A
S
N
E
L
G
G
I
C
Site 41
S402
N
E
L
G
G
I
C
S
I
S
Q
V
E
E
E
Site 42
T415
E
E
M
L
Q
D
N
T
K
S
S
A
Q
P
E
Site 43
S429
E
N
L
I
P
M
W
S
S
D
I
V
T
G
E
Site 44
S430
N
L
I
P
M
W
S
S
D
I
V
T
G
E
K
Site 45
T434
M
W
S
S
D
I
V
T
G
E
K
N
E
P
V
Site 46
S457
E
Q
K
P
K
D
Q
S
L
A
L
S
P
K
L
Site 47
S461
K
D
Q
S
L
A
L
S
P
K
L
E
C
S
G
Site 48
T469
P
K
L
E
C
S
G
T
I
L
A
H
S
N
L
Site 49
S474
S
G
T
I
L
A
H
S
N
L
R
L
L
G
S
Site 50
S481
S
N
L
R
L
L
G
S
S
D
S
P
A
S
A
Site 51
S482
N
L
R
L
L
G
S
S
D
S
P
A
S
A
S
Site 52
S484
R
L
L
G
S
S
D
S
P
A
S
A
S
R
V
Site 53
S487
G
S
S
D
S
P
A
S
A
S
R
V
A
G
I
Site 54
S489
S
D
S
P
A
S
A
S
R
V
A
G
I
T
G
Site 55
S526
P
D
L
Q
P
H
N
S
G
S
E
P
S
L
S
Site 56
S528
L
Q
P
H
N
S
G
S
E
P
S
L
S
R
Q
Site 57
S531
H
N
S
G
S
E
P
S
L
S
R
Q
R
R
Q
Site 58
S533
S
G
S
E
P
S
L
S
R
Q
R
R
Q
K
R
Site 59
T544
R
Q
K
R
R
E
Q
T
E
H
R
G
E
K
R
Site 60
S563
D
L
F
A
F
Q
E
S
P
P
R
F
L
P
S
Site 61
S570
S
P
P
R
F
L
P
S
H
P
I
V
G
K
V
Site 62
T580
I
V
G
K
V
D
V
T
S
T
Q
K
E
A
E
Site 63
S581
V
G
K
V
D
V
T
S
T
Q
K
E
A
E
N
Site 64
T582
G
K
V
D
V
T
S
T
Q
K
E
A
E
N
Q
Site 65
T594
E
N
Q
R
R
V
V
T
G
S
V
S
S
S
R
Site 66
S596
Q
R
R
V
V
T
G
S
V
S
S
S
R
S
S
Site 67
S598
R
V
V
T
G
S
V
S
S
S
R
S
S
E
M
Site 68
S599
V
V
T
G
S
V
S
S
S
R
S
S
E
M
S
Site 69
S600
V
T
G
S
V
S
S
S
R
S
S
E
M
S
S
Site 70
S602
G
S
V
S
S
S
R
S
S
E
M
S
S
S
K
Site 71
S603
S
V
S
S
S
R
S
S
E
M
S
S
S
K
D
Site 72
S606
S
S
R
S
S
E
M
S
S
S
K
D
R
P
L
Site 73
S607
S
R
S
S
E
M
S
S
S
K
D
R
P
L
S
Site 74
S608
R
S
S
E
M
S
S
S
K
D
R
P
L
S
A
Site 75
S614
S
S
K
D
R
P
L
S
A
R
E
R
R
R
L
Site 76
S624
E
R
R
R
L
K
Q
S
Q
E
E
M
S
S
S
Site 77
S629
K
Q
S
Q
E
E
M
S
S
S
G
P
S
V
R
Site 78
S630
Q
S
Q
E
E
M
S
S
S
G
P
S
V
R
K
Site 79
S631
S
Q
E
E
M
S
S
S
G
P
S
V
R
K
A
Site 80
S634
E
M
S
S
S
G
P
S
V
R
K
A
S
L
S
Site 81
S639
G
P
S
V
R
K
A
S
L
S
V
A
G
P
G
Site 82
S641
S
V
R
K
A
S
L
S
V
A
G
P
G
K
P
Site 83
S661
P
L
P
A
R
R
L
S
S
D
C
S
V
T
Q
Site 84
S662
L
P
A
R
R
L
S
S
D
C
S
V
T
Q
E
Site 85
S665
R
R
L
S
S
D
C
S
V
T
Q
E
R
K
Q
Site 86
T667
L
S
S
D
C
S
V
T
Q
E
R
K
Q
I
H
Site 87
S677
R
K
Q
I
H
C
L
S
E
D
E
L
S
S
S
Site 88
S682
C
L
S
E
D
E
L
S
S
S
T
S
S
T
D
Site 89
S683
L
S
E
D
E
L
S
S
S
T
S
S
T
D
K
Site 90
S684
S
E
D
E
L
S
S
S
T
S
S
T
D
K
S
Site 91
S686
D
E
L
S
S
S
T
S
S
T
D
K
S
D
G
Site 92
S687
E
L
S
S
S
T
S
S
T
D
K
S
D
G
D
Site 93
T688
L
S
S
S
T
S
S
T
D
K
S
D
G
D
Y
Site 94
S691
S
T
S
S
T
D
K
S
D
G
D
Y
G
E
G
Site 95
Y695
T
D
K
S
D
G
D
Y
G
E
G
K
G
Q
T
Site 96
S720
T
Q
T
L
K
L
D
S
K
E
S
C
E
D
V
Site 97
S723
L
K
L
D
S
K
E
S
C
E
D
V
P
V
A
Site 98
S734
V
P
V
A
N
P
V
S
E
F
K
L
H
R
K
Site 99
Y742
E
F
K
L
H
R
K
Y
R
D
T
L
I
L
H
Site 100
T745
L
H
R
K
Y
R
D
T
L
I
L
H
G
K
V
Site 101
S772
P
S
A
I
M
P
G
S
E
K
I
R
R
L
V
Site 102
Y822
R
E
H
M
G
E
K
Y
T
T
Y
S
V
K
A
Site 103
T824
H
M
G
E
K
Y
T
T
Y
S
V
K
A
R
Q
Site 104
Y825
M
G
E
K
Y
T
T
Y
S
V
K
A
R
Q
L
Site 105
S826
G
E
K
Y
T
T
Y
S
V
K
A
R
Q
L
K
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation