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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
MAP3K1
Full Name:
Mitogen-activated protein kinase kinase kinase 1
Alias:
EC 2.7.11.25; Kinase MEKK1; M3K1; MAPK/ERK kinase kinase 1; MAPKKK1; MEK kinase 1; MEKK; MEKK 1; MEKK1; Mitogen-activated protein kinase kinase kinase 1
Type:
EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor);
STE
group; STE11 family
Mass (Da):
164470
Number AA:
1512
UniProt ID:
Q13233
International Prot ID:
IPI00012318
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005829
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0000287
GO:0008270
PhosphoSite+
KinaseNET
Biological Process:
GO:0007249
GO:0001747
GO:0030838
Phosida
TranscriptoNet
STRING
Kinexus Products
MAPK/ERK kinase kinase 1; Mitogen-activated protein kinase kinase kinase 1 [Fragment] pan-specific antibody AB-NK107-3#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK107-3
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S10
A
A
A
G
N
R
A
S
S
S
G
F
P
G
A
Site 2
S11
A
A
G
N
R
A
S
S
S
G
F
P
G
A
R
Site 3
S12
A
G
N
R
A
S
S
S
G
F
P
G
A
R
A
Site 4
T20
G
F
P
G
A
R
A
T
S
P
E
A
G
G
G
Site 5
S21
F
P
G
A
R
A
T
S
P
E
A
G
G
G
G
Site 6
S35
G
G
A
L
K
A
S
S
A
P
A
A
A
A
G
Site 7
S49
G
L
L
R
E
A
G
S
G
G
R
E
R
A
D
Site 8
S67
R
Q
L
R
K
V
R
S
V
E
L
D
Q
L
P
Site 9
S83
Q
P
L
F
L
A
A
S
P
P
A
S
S
T
S
Site 10
S87
L
A
A
S
P
P
A
S
S
T
S
P
S
P
E
Site 11
S88
A
A
S
P
P
A
S
S
T
S
P
S
P
E
P
Site 12
S90
S
P
P
A
S
S
T
S
P
S
P
E
P
A
D
Site 13
S92
P
A
S
S
T
S
P
S
P
E
P
A
D
A
A
Site 14
S101
E
P
A
D
A
A
G
S
G
T
G
F
Q
P
V
Site 15
T125
S
R
G
G
A
H
L
T
E
S
V
A
A
P
D
Site 16
S127
G
G
A
H
L
T
E
S
V
A
A
P
D
S
G
Site 17
S133
E
S
V
A
A
P
D
S
G
A
S
S
P
A
A
Site 18
S136
A
A
P
D
S
G
A
S
S
P
A
A
A
E
P
Site 19
S137
A
P
D
S
G
A
S
S
P
A
A
A
E
P
G
Site 20
S154
R
A
P
A
A
E
P
S
P
A
A
A
P
A
G
Site 21
T169
R
E
M
E
N
K
E
T
L
K
G
L
H
K
M
Site 22
S223
I
P
V
K
G
D
G
S
E
M
N
H
L
A
A
Site 23
S232
M
N
H
L
A
A
E
S
P
G
E
V
Q
A
S
Site 24
S239
S
P
G
E
V
Q
A
S
A
A
S
P
A
S
K
Site 25
S242
E
V
Q
A
S
A
A
S
P
A
S
K
G
R
R
Site 26
S245
A
S
A
A
S
P
A
S
K
G
R
R
S
P
S
Site 27
S250
P
A
S
K
G
R
R
S
P
S
P
G
N
S
P
Site 28
S252
S
K
G
R
R
S
P
S
P
G
N
S
P
S
G
Site 29
S256
R
S
P
S
P
G
N
S
P
S
G
R
T
V
K
Site 30
S258
P
S
P
G
N
S
P
S
G
R
T
V
K
S
E
Site 31
T261
G
N
S
P
S
G
R
T
V
K
S
E
S
P
G
Site 32
S264
P
S
G
R
T
V
K
S
E
S
P
G
V
R
R
Site 33
S266
G
R
T
V
K
S
E
S
P
G
V
R
R
K
R
Site 34
S275
G
V
R
R
K
R
V
S
P
V
P
F
Q
S
G
Site 35
S281
V
S
P
V
P
F
Q
S
G
R
I
T
P
P
R
Site 36
T285
P
F
Q
S
G
R
I
T
P
P
R
R
A
P
S
Site 37
S292
T
P
P
R
R
A
P
S
P
D
G
F
S
P
Y
Site 38
S297
A
P
S
P
D
G
F
S
P
Y
S
P
E
E
T
Site 39
Y299
S
P
D
G
F
S
P
Y
S
P
E
E
T
N
R
Site 40
S300
P
D
G
F
S
P
Y
S
P
E
E
T
N
R
R
Site 41
T304
S
P
Y
S
P
E
E
T
N
R
R
V
N
K
V
Site 42
S326
L
Q
Q
I
G
P
N
S
F
L
I
G
G
D
S
Site 43
S333
S
F
L
I
G
G
D
S
P
D
N
K
Y
R
V
Site 44
Y338
G
D
S
P
D
N
K
Y
R
V
F
I
G
P
Q
Site 45
S371
R
V
F
Q
L
E
P
S
D
P
M
L
W
R
K
Site 46
T379
D
P
M
L
W
R
K
T
L
K
N
F
E
V
E
Site 47
S387
L
K
N
F
E
V
E
S
L
F
Q
K
Y
H
S
Site 48
Y392
V
E
S
L
F
Q
K
Y
H
S
R
R
S
S
R
Site 49
S394
S
L
F
Q
K
Y
H
S
R
R
S
S
R
I
K
Site 50
S397
Q
K
Y
H
S
R
R
S
S
R
I
K
A
P
S
Site 51
S398
K
Y
H
S
R
R
S
S
R
I
K
A
P
S
R
Site 52
S404
S
S
R
I
K
A
P
S
R
N
T
I
Q
K
F
Site 53
T407
I
K
A
P
S
R
N
T
I
Q
K
F
V
S
R
Site 54
S413
N
T
I
Q
K
F
V
S
R
M
S
N
S
H
T
Site 55
S416
Q
K
F
V
S
R
M
S
N
S
H
T
L
S
S
Site 56
S418
F
V
S
R
M
S
N
S
H
T
L
S
S
S
S
Site 57
T420
S
R
M
S
N
S
H
T
L
S
S
S
S
T
S
Site 58
S422
M
S
N
S
H
T
L
S
S
S
S
T
S
T
S
Site 59
S423
S
N
S
H
T
L
S
S
S
S
T
S
T
S
S
Site 60
S424
N
S
H
T
L
S
S
S
S
T
S
T
S
S
S
Site 61
S425
S
H
T
L
S
S
S
S
T
S
T
S
S
S
E
Site 62
T426
H
T
L
S
S
S
S
T
S
T
S
S
S
E
N
Site 63
S427
T
L
S
S
S
S
T
S
T
S
S
S
E
N
S
Site 64
T428
L
S
S
S
S
T
S
T
S
S
S
E
N
S
I
Site 65
S429
S
S
S
S
T
S
T
S
S
S
E
N
S
I
K
Site 66
S430
S
S
S
T
S
T
S
S
S
E
N
S
I
K
D
Site 67
S431
S
S
T
S
T
S
S
S
E
N
S
I
K
D
E
Site 68
S434
S
T
S
S
S
E
N
S
I
K
D
E
E
E
Q
Site 69
S455
L
G
M
L
D
E
E
S
L
T
V
C
E
D
G
Site 70
T457
M
L
D
E
E
S
L
T
V
C
E
D
G
C
R
Site 71
Y501
K
W
R
S
H
D
F
Y
S
H
E
L
S
S
P
Site 72
S502
W
R
S
H
D
F
Y
S
H
E
L
S
S
P
V
Site 73
S506
D
F
Y
S
H
E
L
S
S
P
V
D
S
P
S
Site 74
S507
F
Y
S
H
E
L
S
S
P
V
D
S
P
S
S
Site 75
S511
E
L
S
S
P
V
D
S
P
S
S
L
R
A
A
Site 76
S513
S
S
P
V
D
S
P
S
S
L
R
A
A
Q
Q
Site 77
S514
S
P
V
D
S
P
S
S
L
R
A
A
Q
Q
Q
Site 78
S531
Q
Q
Q
P
L
A
G
S
R
R
N
Q
E
S
N
Site 79
S537
G
S
R
R
N
Q
E
S
N
F
N
L
T
H
Y
Site 80
Y544
S
N
F
N
L
T
H
Y
G
T
Q
Q
I
P
P
Site 81
T546
F
N
L
T
H
Y
G
T
Q
Q
I
P
P
A
Y
Site 82
S588
E
M
A
L
R
R
L
S
H
D
V
S
G
A
L
Site 83
S592
R
R
L
S
H
D
V
S
G
A
L
L
L
A
N
Site 84
S602
L
L
L
A
N
G
E
S
T
G
N
S
G
G
S
Site 85
S606
N
G
E
S
T
G
N
S
G
G
S
S
G
S
S
Site 86
S609
S
T
G
N
S
G
G
S
S
G
S
S
P
S
G
Site 87
S610
T
G
N
S
G
G
S
S
G
S
S
P
S
G
G
Site 88
S612
N
S
G
G
S
S
G
S
S
P
S
G
G
A
T
Site 89
S613
S
G
G
S
S
G
S
S
P
S
G
G
A
T
S
Site 90
S615
G
S
S
G
S
S
P
S
G
G
A
T
S
G
S
Site 91
T619
S
S
P
S
G
G
A
T
S
G
S
S
Q
T
S
Site 92
S620
S
P
S
G
G
A
T
S
G
S
S
Q
T
S
I
Site 93
S622
S
G
G
A
T
S
G
S
S
Q
T
S
I
S
G
Site 94
S623
G
G
A
T
S
G
S
S
Q
T
S
I
S
G
D
Site 95
S626
T
S
G
S
S
Q
T
S
I
S
G
D
V
V
E
Site 96
S628
G
S
S
Q
T
S
I
S
G
D
V
V
E
A
C
Site 97
T657
V
Y
V
A
A
L
K
T
L
R
A
M
L
V
Y
Site 98
T665
L
R
A
M
L
V
Y
T
P
C
H
S
L
A
E
Site 99
S696
V
K
C
A
D
A
N
S
R
T
S
Q
L
S
I
Site 100
S699
A
D
A
N
S
R
T
S
Q
L
S
I
S
T
L
Site 101
S702
N
S
R
T
S
Q
L
S
I
S
T
L
L
E
L
Site 102
Y773
L
E
F
P
A
E
F
Y
P
H
I
V
S
T
D
Site 103
S778
E
F
Y
P
H
I
V
S
T
D
V
S
Q
A
E
Site 104
S796
I
R
Y
K
K
L
L
S
L
L
T
F
A
L
Q
Site 105
T799
K
K
L
L
S
L
L
T
F
A
L
Q
S
I
D
Site 106
S808
A
L
Q
S
I
D
N
S
H
S
M
V
G
K
L
Site 107
S810
Q
S
I
D
N
S
H
S
M
V
G
K
L
S
R
Site 108
S816
H
S
M
V
G
K
L
S
R
R
I
Y
L
S
S
Site 109
Y820
G
K
L
S
R
R
I
Y
L
S
S
A
R
M
V
Site 110
S822
L
S
R
R
I
Y
L
S
S
A
R
M
V
T
T
Site 111
S823
S
R
R
I
Y
L
S
S
A
R
M
V
T
T
V
Site 112
T829
S
S
A
R
M
V
T
T
V
P
H
V
F
S
K
Site 113
S844
L
L
E
M
L
S
V
S
S
S
T
H
F
T
R
Site 114
S846
E
M
L
S
V
S
S
S
T
H
F
T
R
M
R
Site 115
T850
V
S
S
S
T
H
F
T
R
M
R
R
R
L
M
Site 116
T876
I
Q
L
G
V
E
D
T
L
D
G
Q
Q
D
S
Site 117
S883
T
L
D
G
Q
Q
D
S
F
L
Q
A
S
V
P
Site 118
S888
Q
D
S
F
L
Q
A
S
V
P
N
N
Y
L
E
Site 119
T896
V
P
N
N
Y
L
E
T
T
E
N
S
S
P
E
Site 120
T897
P
N
N
Y
L
E
T
T
E
N
S
S
P
E
C
Site 121
S901
L
E
T
T
E
N
S
S
P
E
C
T
V
H
L
Site 122
T905
E
N
S
S
P
E
C
T
V
H
L
E
K
T
G
Site 123
T918
T
G
K
G
L
C
A
T
K
L
S
A
S
S
E
Site 124
S921
G
L
C
A
T
K
L
S
A
S
S
E
D
I
S
Site 125
S923
C
A
T
K
L
S
A
S
S
E
D
I
S
E
R
Site 126
S924
A
T
K
L
S
A
S
S
E
D
I
S
E
R
L
Site 127
S928
S
A
S
S
E
D
I
S
E
R
L
A
S
I
S
Site 128
S933
D
I
S
E
R
L
A
S
I
S
V
G
P
S
S
Site 129
S935
S
E
R
L
A
S
I
S
V
G
P
S
S
S
T
Site 130
S939
A
S
I
S
V
G
P
S
S
S
T
T
T
T
T
Site 131
S940
S
I
S
V
G
P
S
S
S
T
T
T
T
T
T
Site 132
S941
I
S
V
G
P
S
S
S
T
T
T
T
T
T
T
Site 133
T943
V
G
P
S
S
S
T
T
T
T
T
T
T
T
E
Site 134
T944
G
P
S
S
S
T
T
T
T
T
T
T
T
E
Q
Site 135
T945
P
S
S
S
T
T
T
T
T
T
T
T
E
Q
P
Site 136
T949
T
T
T
T
T
T
T
T
E
Q
P
K
P
M
V
Site 137
S964
Q
T
K
G
R
P
H
S
Q
C
L
N
S
S
P
Site 138
S969
P
H
S
Q
C
L
N
S
S
P
L
S
H
H
S
Site 139
S970
H
S
Q
C
L
N
S
S
P
L
S
H
H
S
Q
Site 140
S973
C
L
N
S
S
P
L
S
H
H
S
Q
L
M
F
Site 141
S976
S
S
P
L
S
H
H
S
Q
L
M
F
P
A
L
Site 142
S984
Q
L
M
F
P
A
L
S
T
P
S
S
S
T
P
Site 143
T985
L
M
F
P
A
L
S
T
P
S
S
S
T
P
S
Site 144
S988
P
A
L
S
T
P
S
S
S
T
P
S
V
P
A
Site 145
S989
A
L
S
T
P
S
S
S
T
P
S
V
P
A
G
Site 146
T990
L
S
T
P
S
S
S
T
P
S
V
P
A
G
T
Site 147
S992
T
P
S
S
S
T
P
S
V
P
A
G
T
A
T
Site 148
T999
S
V
P
A
G
T
A
T
D
V
S
K
H
R
L
Site 149
S1002
A
G
T
A
T
D
V
S
K
H
R
L
Q
G
F
Site 150
S1016
F
I
P
C
R
I
P
S
A
S
P
Q
T
Q
R
Site 151
S1018
P
C
R
I
P
S
A
S
P
Q
T
Q
R
K
F
Site 152
T1021
I
P
S
A
S
P
Q
T
Q
R
K
F
S
L
Q
Site 153
S1026
P
Q
T
Q
R
K
F
S
L
Q
F
H
R
N
C
Site 154
S1039
N
C
P
E
N
K
D
S
D
K
L
S
P
V
F
Site 155
S1043
N
K
D
S
D
K
L
S
P
V
F
T
Q
S
R
Site 156
T1047
D
K
L
S
P
V
F
T
Q
S
R
P
L
P
S
Site 157
S1049
L
S
P
V
F
T
Q
S
R
P
L
P
S
S
N
Site 158
S1055
Q
S
R
P
L
P
S
S
N
I
H
R
P
K
P
Site 159
T1066
R
P
K
P
S
R
P
T
P
G
N
T
S
K
Q
Site 160
S1071
R
P
T
P
G
N
T
S
K
Q
G
D
P
S
K
Site 161
S1077
T
S
K
Q
G
D
P
S
K
N
S
M
T
L
D
Site 162
T1082
D
P
S
K
N
S
M
T
L
D
L
N
S
S
S
Site 163
S1087
S
M
T
L
D
L
N
S
S
S
K
C
D
D
S
Site 164
S1088
M
T
L
D
L
N
S
S
S
K
C
D
D
S
F
Site 165
S1089
S
M
T
L
D
L
N
S
S
S
K
C
D
D
S
Site 166
S1094
S
S
S
K
C
D
D
S
F
G
C
S
S
N
S
Site 167
S1098
C
D
D
S
F
G
C
S
S
N
S
S
N
A
V
Site 168
S1099
D
D
S
F
G
C
S
S
N
S
S
N
A
V
I
Site 169
S1101
S
F
G
C
S
S
N
S
S
N
A
V
I
P
S
Site 170
S1102
F
G
C
S
S
N
S
S
N
A
V
I
P
S
D
Site 171
S1108
S
S
N
A
V
I
P
S
D
E
T
V
F
T
P
Site 172
T1114
P
S
D
E
T
V
F
T
P
V
E
E
K
C
R
Site 173
T1126
K
C
R
L
D
V
N
T
E
L
N
S
S
I
E
Site 174
S1131
V
N
T
E
L
N
S
S
I
E
D
L
L
E
A
Site 175
S1139
I
E
D
L
L
E
A
S
M
P
S
S
D
T
T
Site 176
S1143
L
E
A
S
M
P
S
S
D
T
T
V
T
F
K
Site 177
T1145
A
S
M
P
S
S
D
T
T
V
T
F
K
S
E
Site 178
T1146
S
M
P
S
S
D
T
T
V
T
F
K
S
E
V
Site 179
T1148
P
S
S
D
T
T
V
T
F
K
S
E
V
A
V
Site 180
S1157
K
S
E
V
A
V
L
S
P
E
K
A
E
N
D
Site 181
T1166
E
K
A
E
N
D
D
T
Y
K
D
D
V
N
H
Site 182
Y1167
K
A
E
N
D
D
T
Y
K
D
D
V
N
H
N
Site 183
T1223
I
I
I
I
Q
Q
D
T
P
E
T
L
P
G
H
Site 184
T1241
K
Q
P
Y
R
E
D
T
E
W
L
K
G
Q
Q
Site 185
S1255
Q
I
G
L
G
A
F
S
S
C
Y
Q
A
Q
D
Site 186
Y1258
L
G
A
F
S
S
C
Y
Q
A
Q
D
V
G
T
Site 187
Y1276
M
A
V
K
Q
V
T
Y
V
R
N
T
S
S
E
Site 188
T1280
Q
V
T
Y
V
R
N
T
S
S
E
Q
E
E
V
Site 189
S1281
V
T
Y
V
R
N
T
S
S
E
Q
E
E
V
V
Site 190
T1313
I
I
R
M
L
G
A
T
C
E
K
S
N
Y
N
Site 191
S1336
G
S
V
A
H
L
L
S
K
Y
G
A
F
K
E
Site 192
Y1338
V
A
H
L
L
S
K
Y
G
A
F
K
E
S
V
Site 193
S1344
K
Y
G
A
F
K
E
S
V
V
I
N
Y
T
E
Site 194
S1358
E
Q
L
L
R
G
L
S
Y
L
H
E
N
Q
I
Site 195
S1397
G
A
A
A
R
L
A
S
K
G
T
G
A
G
E
Site 196
T1400
A
R
L
A
S
K
G
T
G
A
G
E
F
Q
G
Site 197
T1412
F
Q
G
Q
L
L
G
T
I
A
F
M
A
P
E
Site 198
Y1426
E
V
L
R
G
Q
Q
Y
G
R
S
C
D
V
W
Site 199
S1466
A
L
I
F
K
I
A
S
A
T
T
A
P
S
I
Site 200
T1469
F
K
I
A
S
A
T
T
A
P
S
I
P
S
H
Site 201
S1472
A
S
A
T
T
A
P
S
I
P
S
H
L
S
P
Site 202
S1475
T
T
A
P
S
I
P
S
H
L
S
P
G
L
R
Site 203
S1478
P
S
I
P
S
H
L
S
P
G
L
R
D
V
A
Site 204
S1499
Q
P
Q
D
R
P
P
S
R
E
L
L
K
H
P
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation