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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
smMLCK
Full Name:
Myosin light chain kinase, smooth muscle
Alias:
EC 2.7.11.18; KMLS; KRP; MLCK; MLCK1; MYLK; MYLK1; Myosin light chain kinase; Myosin light polypeptide kinase; SmMLCK; Smooth muscle myosin light chain kinase; Telokin
Type:
EC 2.7.11.18; Protein kinase, Ser/Thr (non-receptor);
CAMK
group; MLCK family
Mass (Da):
210774
Number AA:
1914
UniProt ID:
Q15746
International Prot ID:
IPI00336081
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0003779
GO:0005509
PhosphoSite+
KinaseNET
Biological Process:
GO:0006468
Phosida
TranscriptoNet
STRING
Kinexus Products
MYLKSelectide - MYLK2 (skMLCK) protein kinase substrate peptide - Powder PE-01BIE95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01BIE95
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S9
G
D
V
K
L
V
A
S
S
H
I
S
K
T
S
Site 2
S13
L
V
A
S
S
H
I
S
K
T
S
L
S
V
D
Site 3
S16
S
S
H
I
S
K
T
S
L
S
V
D
P
S
R
Site 4
S18
H
I
S
K
T
S
L
S
V
D
P
S
R
V
D
Site 5
S22
T
S
L
S
V
D
P
S
R
V
D
S
M
P
L
Site 6
S26
V
D
P
S
R
V
D
S
M
P
L
T
E
A
P
Site 7
Y59
F
E
G
R
V
R
G
Y
P
E
P
Q
V
T
W
Site 8
S75
R
N
G
Q
P
I
T
S
G
G
R
F
L
L
D
Site 9
T88
L
D
C
G
I
R
G
T
F
S
L
V
I
H
A
Site 10
Y104
H
E
E
D
R
G
K
Y
T
C
E
A
T
N
G
Site 11
T109
G
K
Y
T
C
E
A
T
N
G
S
G
A
R
Q
Site 12
T118
G
S
G
A
R
Q
V
T
V
E
L
T
V
E
G
Site 13
T122
R
Q
V
T
V
E
L
T
V
E
G
S
F
A
K
Site 14
S126
V
E
L
T
V
E
G
S
F
A
K
Q
L
G
Q
Site 15
T139
G
Q
P
V
V
S
K
T
L
G
D
R
F
S
A
Site 16
S145
K
T
L
G
D
R
F
S
A
S
A
V
E
T
R
Site 17
S147
L
G
D
R
F
S
A
S
A
V
E
T
R
P
S
Site 18
T151
F
S
A
S
A
V
E
T
R
P
S
I
W
G
E
Site 19
S154
S
A
V
E
T
R
P
S
I
W
G
E
C
P
P
Site 20
S181
E
G
Q
M
G
R
F
S
C
K
I
T
G
R
P
Site 21
T185
G
R
F
S
C
K
I
T
G
R
P
Q
P
Q
V
Site 22
S204
G
N
V
P
L
Q
P
S
A
R
V
S
V
S
E
Site 23
S208
L
Q
P
S
A
R
V
S
V
S
E
K
N
G
M
Site 24
S210
P
S
A
R
V
S
V
S
E
K
N
G
M
Q
V
Site 25
Y231
N
Q
D
D
V
G
V
Y
T
C
L
V
V
N
G
Site 26
S243
V
N
G
S
G
K
A
S
M
S
A
E
L
S
I
Site 27
S245
G
S
G
K
A
S
M
S
A
E
L
S
I
Q
G
Site 28
S255
L
S
I
Q
G
L
D
S
A
N
R
S
F
V
R
Site 29
S259
G
L
D
S
A
N
R
S
F
V
R
E
T
K
A
Site 30
T264
N
R
S
F
V
R
E
T
K
A
T
N
S
D
V
Site 31
T267
F
V
R
E
T
K
A
T
N
S
D
V
R
K
E
Site 32
S269
R
E
T
K
A
T
N
S
D
V
R
K
E
V
T
Site 33
T276
S
D
V
R
K
E
V
T
N
V
I
S
K
E
S
Site 34
S280
K
E
V
T
N
V
I
S
K
E
S
K
L
D
S
Site 35
S283
T
N
V
I
S
K
E
S
K
L
D
S
L
E
A
Site 36
S287
S
K
E
S
K
L
D
S
L
E
A
A
A
K
S
Site 37
S294
S
L
E
A
A
A
K
S
K
N
C
S
S
P
Q
Site 38
S298
A
A
K
S
K
N
C
S
S
P
Q
R
G
G
S
Site 39
S299
A
K
S
K
N
C
S
S
P
Q
R
G
G
S
P
Site 40
S305
S
S
P
Q
R
G
G
S
P
P
W
A
A
N
S
Site 41
S312
S
P
P
W
A
A
N
S
Q
P
Q
P
P
R
E
Site 42
S320
Q
P
Q
P
P
R
E
S
K
L
E
S
C
K
D
Site 43
S324
P
R
E
S
K
L
E
S
C
K
D
S
P
R
T
Site 44
S328
K
L
E
S
C
K
D
S
P
R
T
A
P
Q
T
Site 45
T331
S
C
K
D
S
P
R
T
A
P
Q
T
P
V
L
Site 46
T335
S
P
R
T
A
P
Q
T
P
V
L
Q
K
T
S
Site 47
T341
Q
T
P
V
L
Q
K
T
S
S
S
I
T
L
Q
Site 48
S343
P
V
L
Q
K
T
S
S
S
I
T
L
Q
A
A
Site 49
S344
V
L
Q
K
T
S
S
S
I
T
L
Q
A
A
R
Site 50
T346
Q
K
T
S
S
S
I
T
L
Q
A
A
R
V
Q
Site 51
S365
A
P
G
L
G
V
L
S
P
S
G
E
E
R
K
Site 52
T381
P
A
P
P
R
P
A
T
F
P
T
R
Q
P
G
Site 53
T384
P
R
P
A
T
F
P
T
R
Q
P
G
L
G
S
Site 54
S391
T
R
Q
P
G
L
G
S
Q
D
V
V
S
K
A
Site 55
S396
L
G
S
Q
D
V
V
S
K
A
A
N
R
R
I
Site 56
S411
P
M
E
G
Q
R
D
S
A
F
P
K
F
E
S
Site 57
S418
S
A
F
P
K
F
E
S
K
P
Q
S
Q
E
V
Site 58
S422
K
F
E
S
K
P
Q
S
Q
E
V
K
E
N
Q
Site 59
S438
V
K
F
R
C
E
V
S
G
I
P
K
P
E
V
Site 60
T452
V
A
W
F
L
E
G
T
P
V
R
R
Q
E
G
Site 61
S460
P
V
R
R
Q
E
G
S
I
E
V
Y
E
D
A
Site 62
Y464
Q
E
G
S
I
E
V
Y
E
D
A
G
S
H
Y
Site 63
S469
E
V
Y
E
D
A
G
S
H
Y
L
C
L
L
K
Site 64
Y471
Y
E
D
A
G
S
H
Y
L
C
L
L
K
A
R
Site 65
S482
L
K
A
R
T
R
D
S
G
T
Y
S
C
T
A
Site 66
T484
A
R
T
R
D
S
G
T
Y
S
C
T
A
S
N
Site 67
Y485
R
T
R
D
S
G
T
Y
S
C
T
A
S
N
A
Site 68
S486
T
R
D
S
G
T
Y
S
C
T
A
S
N
A
Q
Site 69
S490
G
T
Y
S
C
T
A
S
N
A
Q
G
Q
V
S
Site 70
S497
S
N
A
Q
G
Q
V
S
C
S
W
T
L
Q
V
Site 71
T501
G
Q
V
S
C
S
W
T
L
Q
V
E
R
L
A
Site 72
S515
A
V
M
E
V
A
P
S
F
S
S
V
L
K
D
Site 73
T540
L
Q
C
S
V
R
G
T
P
V
P
R
I
T
W
Site 74
T546
G
T
P
V
P
R
I
T
W
L
L
N
G
Q
P
Site 75
Y556
L
N
G
Q
P
I
Q
Y
A
R
S
T
C
E
A
Site 76
T560
P
I
Q
Y
A
R
S
T
C
E
A
G
V
A
E
Site 77
T580
A
L
P
E
D
H
G
T
Y
T
C
L
A
E
N
Site 78
T599
V
S
C
S
A
W
V
T
V
H
E
K
K
S
S
Site 79
S605
V
T
V
H
E
K
K
S
S
R
K
S
E
Y
L
Site 80
S609
E
K
K
S
S
R
K
S
E
Y
L
L
P
V
A
Site 81
Y611
K
S
S
R
K
S
E
Y
L
L
P
V
A
P
S
Site 82
S638
D
L
K
V
M
D
G
S
Q
V
T
M
T
V
Q
Site 83
T643
D
G
S
Q
V
T
M
T
V
Q
V
S
G
N
P
Site 84
S647
V
T
M
T
V
Q
V
S
G
N
P
P
P
E
V
Site 85
T711
V
R
T
Q
A
V
L
T
V
Q
E
P
H
D
G
Site 86
T719
V
Q
E
P
H
D
G
T
Q
P
W
F
I
S
K
Site 87
S725
G
T
Q
P
W
F
I
S
K
P
R
S
V
T
A
Site 88
S729
W
F
I
S
K
P
R
S
V
T
A
S
L
G
Q
Site 89
T731
I
S
K
P
R
S
V
T
A
S
L
G
Q
S
V
Site 90
T751
I
A
G
D
P
F
P
T
V
H
W
L
R
D
G
Site 91
Y792
Q
P
W
H
A
G
Q
Y
E
I
L
L
K
N
R
Site 92
S804
K
N
R
V
G
E
C
S
C
Q
V
S
L
M
L
Site 93
S808
G
E
C
S
C
Q
V
S
L
M
L
Q
N
S
S
Site 94
S814
V
S
L
M
L
Q
N
S
S
A
R
A
L
P
R
Site 95
S815
S
L
M
L
Q
N
S
S
A
R
A
L
P
R
G
Site 96
S827
P
R
G
R
E
P
A
S
C
E
D
L
C
G
G
Site 97
S843
V
G
A
D
G
G
G
S
D
R
Y
G
S
L
R
Site 98
Y846
D
G
G
G
S
D
R
Y
G
S
L
R
P
G
W
Site 99
S848
G
G
S
D
R
Y
G
S
L
R
P
G
W
P
A
Site 100
T879
V
L
K
R
R
V
E
T
R
Q
H
T
E
E
A
Site 101
T883
R
V
E
T
R
Q
H
T
E
E
A
I
R
Q
Q
Site 102
S906
D
L
L
G
K
K
V
S
T
K
T
L
S
E
D
Site 103
T909
G
K
K
V
S
T
K
T
L
S
E
D
D
L
K
Site 104
T937
Q
R
Q
V
K
P
K
T
V
S
E
E
E
R
K
Site 105
S947
E
E
E
R
K
V
H
S
P
Q
Q
V
D
F
R
Site 106
S955
P
Q
Q
V
D
F
R
S
V
L
A
K
K
G
T
Site 107
S963
V
L
A
K
K
G
T
S
K
T
P
V
P
E
K
Site 108
T965
A
K
K
G
T
S
K
T
P
V
P
E
K
V
P
Site 109
T978
V
P
P
P
K
P
A
T
P
D
F
R
S
V
L
Site 110
S983
P
A
T
P
D
F
R
S
V
L
G
G
K
K
K
Site 111
S998
L
P
A
E
N
G
S
S
S
A
E
T
L
N
A
Site 112
S999
P
A
E
N
G
S
S
S
A
E
T
L
N
A
K
Site 113
T1002
N
G
S
S
S
A
E
T
L
N
A
K
A
V
E
Site 114
S1010
L
N
A
K
A
V
E
S
S
K
P
L
S
N
A
Site 115
S1011
N
A
K
A
V
E
S
S
K
P
L
S
N
A
Q
Site 116
S1015
V
E
S
S
K
P
L
S
N
A
Q
P
S
G
P
Site 117
S1020
P
L
S
N
A
Q
P
S
G
P
L
K
P
V
G
Site 118
S1061
K
P
D
E
N
L
K
S
A
S
K
E
E
L
K
Site 119
T1084
C
K
R
G
H
A
G
T
T
D
N
E
K
R
S
Site 120
S1091
T
T
D
N
E
K
R
S
E
S
Q
G
T
A
P
Site 121
S1093
D
N
E
K
R
S
E
S
Q
G
T
A
P
A
F
Site 122
T1096
K
R
S
E
S
Q
G
T
A
P
A
F
K
Q
K
Site 123
T1132
P
P
A
T
I
I
W
T
L
N
G
K
T
L
K
Site 124
S1154
S
Q
E
G
S
L
C
S
V
S
I
E
K
A
L
Site 125
S1156
E
G
S
L
C
S
V
S
I
E
K
A
L
P
E
Site 126
Y1168
L
P
E
D
R
G
L
Y
K
C
V
A
K
N
D
Site 127
S1182
D
A
G
Q
A
E
C
S
C
Q
V
T
V
D
D
Site 128
T1186
A
E
C
S
C
Q
V
T
V
D
D
A
P
A
S
Site 129
S1193
T
V
D
D
A
P
A
S
E
N
T
K
A
P
E
Site 130
S1203
T
K
A
P
E
M
K
S
R
R
P
K
S
S
L
Site 131
S1208
M
K
S
R
R
P
K
S
S
L
P
P
V
L
G
Site 132
S1209
K
S
R
R
P
K
S
S
L
P
P
V
L
G
T
Site 133
T1221
L
G
T
E
S
D
A
T
V
K
K
K
P
A
P
Site 134
T1230
K
K
K
P
A
P
K
T
P
P
K
A
A
M
P
Site 135
S1254
Q
K
V
R
A
G
E
S
V
E
L
F
G
K
V
Site 136
S1293
V
E
N
S
E
N
G
S
K
L
T
I
L
A
A
Site 137
T1326
R
Q
A
Q
V
N
L
T
V
V
D
K
P
D
P
Site 138
T1337
K
P
D
P
P
A
G
T
P
C
A
S
D
I
R
Site 139
S1341
P
A
G
T
P
C
A
S
D
I
R
S
S
S
L
Site 140
S1345
P
C
A
S
D
I
R
S
S
S
L
T
L
S
W
Site 141
S1346
C
A
S
D
I
R
S
S
S
L
T
L
S
W
Y
Site 142
S1347
A
S
D
I
R
S
S
S
L
T
L
S
W
Y
G
Site 143
T1349
D
I
R
S
S
S
L
T
L
S
W
Y
G
S
S
Site 144
S1351
R
S
S
S
L
T
L
S
W
Y
G
S
S
Y
D
Site 145
Y1353
S
S
L
T
L
S
W
Y
G
S
S
Y
D
G
G
Site 146
S1355
L
T
L
S
W
Y
G
S
S
Y
D
G
G
S
A
Site 147
S1356
T
L
S
W
Y
G
S
S
Y
D
G
G
S
A
V
Site 148
Y1357
L
S
W
Y
G
S
S
Y
D
G
G
S
A
V
Q
Site 149
S1361
G
S
S
Y
D
G
G
S
A
V
Q
S
Y
S
I
Site 150
S1365
D
G
G
S
A
V
Q
S
Y
S
I
E
I
W
D
Site 151
S1367
G
S
A
V
Q
S
Y
S
I
E
I
W
D
S
A
Site 152
T1377
I
W
D
S
A
N
K
T
W
K
E
L
A
T
C
Site 153
T1383
K
T
W
K
E
L
A
T
C
R
S
T
S
F
N
Site 154
S1386
K
E
L
A
T
C
R
S
T
S
F
N
V
Q
D
Site 155
T1387
E
L
A
T
C
R
S
T
S
F
N
V
Q
D
L
Site 156
S1388
L
A
T
C
R
S
T
S
F
N
V
Q
D
L
L
Site 157
Y1400
D
L
L
P
D
H
E
Y
K
F
R
V
R
A
I
Site 158
Y1410
R
V
R
A
I
N
V
Y
G
T
S
E
P
S
Q
Site 159
T1412
R
A
I
N
V
Y
G
T
S
E
P
S
Q
E
S
Site 160
S1416
V
Y
G
T
S
E
P
S
Q
E
S
E
L
T
T
Site 161
T1422
P
S
Q
E
S
E
L
T
T
V
G
E
K
P
E
Site 162
T1423
S
Q
E
S
E
L
T
T
V
G
E
K
P
E
E
Site 163
S1438
P
K
D
E
V
E
V
S
D
D
D
E
K
E
P
Site 164
Y1449
E
K
E
P
E
V
D
Y
R
T
V
T
I
N
T
Site 165
T1451
E
P
E
V
D
Y
R
T
V
T
I
N
T
E
Q
Site 166
T1453
E
V
D
Y
R
T
V
T
I
N
T
E
Q
K
V
Site 167
S1461
I
N
T
E
Q
K
V
S
D
F
Y
D
I
E
E
Site 168
Y1464
E
Q
K
V
S
D
F
Y
D
I
E
E
R
L
G
Site 169
S1472
D
I
E
E
R
L
G
S
G
K
F
G
Q
V
F
Site 170
Y1498
A
G
K
F
F
K
A
Y
S
A
K
E
K
E
N
Site 171
S1499
G
K
F
F
K
A
Y
S
A
K
E
K
E
N
I
Site 172
T1558
I
D
E
D
F
E
L
T
E
R
E
C
I
K
Y
Site 173
Y1565
T
E
R
E
C
I
K
Y
M
R
Q
I
S
E
G
Site 174
Y1575
Q
I
S
E
G
V
E
Y
I
H
K
Q
G
I
V
Site 175
S1617
R
R
L
E
N
A
G
S
L
K
V
L
F
G
T
Site 176
Y1635
V
A
P
E
V
I
N
Y
E
P
I
G
Y
A
T
Site 177
Y1640
I
N
Y
E
P
I
G
Y
A
T
D
M
W
S
I
Site 178
T1669
M
G
D
N
D
N
E
T
L
A
N
V
T
S
A
Site 179
S1689
D
E
A
F
D
E
I
S
D
D
A
K
D
F
I
Site 180
S1697
D
D
A
K
D
F
I
S
N
L
L
K
K
D
M
Site 181
S1732
N
M
E
A
K
K
L
S
K
D
R
M
K
K
Y
Site 182
Y1739
S
K
D
R
M
K
K
Y
M
A
R
R
K
W
Q
Site 183
T1748
A
R
R
K
W
Q
K
T
G
N
A
V
R
A
I
Site 184
S1759
V
R
A
I
G
R
L
S
S
M
A
M
I
S
G
Site 185
S1760
R
A
I
G
R
L
S
S
M
A
M
I
S
G
L
Site 186
S1765
L
S
S
M
A
M
I
S
G
L
S
G
R
K
S
Site 187
S1768
M
A
M
I
S
G
L
S
G
R
K
S
S
T
G
Site 188
S1772
S
G
L
S
G
R
K
S
S
T
G
S
P
T
S
Site 189
S1773
G
L
S
G
R
K
S
S
T
G
S
P
T
S
P
Site 190
T1774
L
S
G
R
K
S
S
T
G
S
P
T
S
P
L
Site 191
S1776
G
R
K
S
S
T
G
S
P
T
S
P
L
N
A
Site 192
T1778
K
S
S
T
G
S
P
T
S
P
L
N
A
E
K
Site 193
S1779
S
S
T
G
S
P
T
S
P
L
N
A
E
K
L
Site 194
S1788
L
N
A
E
K
L
E
S
E
E
D
V
S
Q
A
Site 195
S1793
L
E
S
E
E
D
V
S
Q
A
F
L
E
A
V
Site 196
Y1810
E
K
P
H
V
K
P
Y
F
S
K
T
I
R
D
Site 197
S1812
P
H
V
K
P
Y
F
S
K
T
I
R
D
L
E
Site 198
T1814
V
K
P
Y
F
S
K
T
I
R
D
L
E
V
V
Site 199
S1824
D
L
E
V
V
E
G
S
A
A
R
F
D
C
K
Site 200
Y1835
F
D
C
K
I
E
G
Y
P
D
P
E
V
V
W
Site 201
S1852
D
D
Q
S
I
R
E
S
R
H
F
Q
I
D
Y
Site 202
Y1859
S
R
H
F
Q
I
D
Y
D
E
D
G
N
C
S
Site 203
S1870
G
N
C
S
L
I
I
S
D
V
C
G
D
D
D
Site 204
Y1880
C
G
D
D
D
A
K
Y
T
C
K
A
V
N
S
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation