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Updated November 2019
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Protein Info
Short Name:
ARFGEF3
Full Name:
Brefeldin A-inhibited guanine nucleotide-exchange protein 3
Alias:
BIG3; C6orf92; DJ171N11.1; KIAA1244
Type:
Guanine nucleotide exchange factor, ARF; Membrane protein, integral
Mass (Da):
240650
Number AA:
UniProt ID:
Q5TH69
International Prot ID:
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0016021
GO:0005622
Uniprot
OncoNet
Molecular Function:
GO:0005086
PhosphoSite+
KinaseNET
Biological Process:
GO:0032012
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S13
R
K
L
Q
K
E
A
S
G
S
K
Y
K
A
I
Site 2
S15
L
Q
K
E
A
S
G
S
K
Y
K
A
I
K
E
Site 3
Y17
K
E
A
S
G
S
K
Y
K
A
I
K
E
S
C
Site 4
S59
P
L
Q
L
A
L
E
S
K
N
V
K
L
A
Q
Site 5
S77
A
G
M
Q
K
L
L
S
E
E
R
F
V
S
M
Site 6
S83
L
S
E
E
R
F
V
S
M
E
T
D
S
D
E
Site 7
S88
F
V
S
M
E
T
D
S
D
E
K
Q
L
L
N
Site 8
Y124
K
V
L
L
C
I
T
Y
T
P
T
F
D
L
N
Site 9
Y146
A
E
V
C
I
E
T
Y
I
S
S
C
H
Q
R
Site 10
T171
S
Q
M
L
S
D
L
T
L
Q
L
R
Q
R
Q
Site 11
S197
Q
D
F
G
N
Q
G
S
T
V
E
S
L
C
D
Site 12
S201
N
Q
G
S
T
V
E
S
L
C
D
D
V
V
S
Site 13
S224
L
Q
A
A
I
N
D
S
Q
Q
L
Q
L
L
Y
Site 14
S243
L
S
V
L
S
S
S
S
S
S
M
H
L
H
R
Site 15
S244
S
V
L
S
S
S
S
S
S
M
H
L
H
R
R
Site 16
S245
V
L
S
S
S
S
S
S
M
H
L
H
R
R
F
Site 17
T276
G
N
P
I
H
D
K
T
I
T
S
A
H
T
S
Site 18
T278
P
I
H
D
K
T
I
T
S
A
H
T
S
S
T
Site 19
S279
I
H
D
K
T
I
T
S
A
H
T
S
S
T
S
Site 20
T282
K
T
I
T
S
A
H
T
S
S
T
S
T
S
L
Site 21
S283
T
I
T
S
A
H
T
S
S
T
S
T
S
L
E
Site 22
S284
I
T
S
A
H
T
S
S
T
S
T
S
L
E
S
Site 23
S286
S
A
H
T
S
S
T
S
T
S
L
E
S
D
S
Site 24
T287
A
H
T
S
S
T
S
T
S
L
E
S
D
S
A
Site 25
S288
H
T
S
S
T
S
T
S
L
E
S
D
S
A
S
Site 26
S291
S
T
S
T
S
L
E
S
D
S
A
S
P
G
V
Site 27
S293
S
T
S
L
E
S
D
S
A
S
P
G
V
S
D
Site 28
S295
S
L
E
S
D
S
A
S
P
G
V
S
D
H
G
Site 29
S299
D
S
A
S
P
G
V
S
D
H
G
R
G
S
G
Site 30
S305
V
S
D
H
G
R
G
S
G
C
S
C
T
A
P
Site 31
S308
H
G
R
G
S
G
C
S
C
T
A
P
A
L
S
Site 32
T310
R
G
S
G
C
S
C
T
A
P
A
L
S
G
P
Site 33
S338
R
L
V
G
S
V
D
S
M
K
P
V
L
Q
S
Site 34
Y353
L
Y
H
R
V
L
L
Y
P
P
P
Q
H
R
V
Site 35
S383
L
C
D
L
A
G
P
S
S
T
E
S
E
S
R
Site 36
S384
C
D
L
A
G
P
S
S
T
E
S
E
S
R
K
Site 37
T385
D
L
A
G
P
S
S
T
E
S
E
S
R
K
R
Site 38
S387
A
G
P
S
S
T
E
S
E
S
R
K
R
S
I
Site 39
S389
P
S
S
T
E
S
E
S
R
K
R
S
I
S
K
Site 40
S393
E
S
E
S
R
K
R
S
I
S
K
R
K
S
H
Site 41
S395
E
S
R
K
R
S
I
S
K
R
K
S
H
L
D
Site 42
S399
R
S
I
S
K
R
K
S
H
L
D
L
L
K
L
Site 43
S441
L
G
A
L
D
E
L
S
Q
G
K
G
L
S
E
Site 44
S468
L
K
D
G
A
E
W
S
R
D
S
M
E
I
N
Site 45
S471
G
A
E
W
S
R
D
S
M
E
I
N
E
A
D
Site 46
S487
R
W
Q
R
R
V
L
S
S
E
H
T
P
W
E
Site 47
S488
W
Q
R
R
V
L
S
S
E
H
T
P
W
E
S
Site 48
T491
R
V
L
S
S
E
H
T
P
W
E
S
G
N
E
Site 49
S500
W
E
S
G
N
E
R
S
L
D
I
S
I
S
V
Site 50
S504
N
E
R
S
L
D
I
S
I
S
V
T
T
D
T
Site 51
S506
R
S
L
D
I
S
I
S
V
T
T
D
T
G
Q
Site 52
T508
L
D
I
S
I
S
V
T
T
D
T
G
Q
T
T
Site 53
T511
S
I
S
V
T
T
D
T
G
Q
T
T
L
E
G
Site 54
T515
T
T
D
T
G
Q
T
T
L
E
G
E
L
G
Q
Site 55
T523
L
E
G
E
L
G
Q
T
T
P
E
D
H
S
G
Site 56
T524
E
G
E
L
G
Q
T
T
P
E
D
H
S
G
N
Site 57
S529
Q
T
T
P
E
D
H
S
G
N
H
K
N
S
L
Site 58
S535
H
S
G
N
H
K
N
S
L
K
S
P
A
I
P
Site 59
S538
N
H
K
N
S
L
K
S
P
A
I
P
E
G
K
Site 60
T547
A
I
P
E
G
K
E
T
L
S
K
V
L
E
T
Site 61
S549
P
E
G
K
E
T
L
S
K
V
L
E
T
E
A
Site 62
T568
D
V
V
Q
R
S
H
T
V
P
Y
P
D
I
T
Site 63
Y571
Q
R
S
H
T
V
P
Y
P
D
I
T
N
F
L
Site 64
S579
P
D
I
T
N
F
L
S
V
D
C
R
T
R
S
Site 65
T584
F
L
S
V
D
C
R
T
R
S
Y
G
S
R
Y
Site 66
S586
S
V
D
C
R
T
R
S
Y
G
S
R
Y
S
E
Site 67
Y587
V
D
C
R
T
R
S
Y
G
S
R
Y
S
E
S
Site 68
S589
C
R
T
R
S
Y
G
S
R
Y
S
E
S
N
F
Site 69
S592
R
S
Y
G
S
R
Y
S
E
S
N
F
S
V
D
Site 70
S594
Y
G
S
R
Y
S
E
S
N
F
S
V
D
D
Q
Site 71
S597
R
Y
S
E
S
N
F
S
V
D
D
Q
D
L
S
Site 72
S604
S
V
D
D
Q
D
L
S
R
T
E
F
D
S
C
Site 73
T606
D
D
Q
D
L
S
R
T
E
F
D
S
C
D
Q
Site 74
S610
L
S
R
T
E
F
D
S
C
D
Q
Y
S
M
A
Site 75
Y614
E
F
D
S
C
D
Q
Y
S
M
A
A
E
K
D
Site 76
S622
S
M
A
A
E
K
D
S
G
R
S
D
V
S
D
Site 77
S628
D
S
G
R
S
D
V
S
D
I
G
S
D
N
C
Site 78
S632
S
D
V
S
D
I
G
S
D
N
C
S
L
A
D
Site 79
S636
D
I
G
S
D
N
C
S
L
A
D
E
E
Q
T
Site 80
T643
S
L
A
D
E
E
Q
T
P
R
D
C
L
G
H
Site 81
S652
R
D
C
L
G
H
R
S
L
R
T
A
A
L
S
Site 82
S659
S
L
R
T
A
A
L
S
L
K
L
L
K
N
Q
Site 83
S672
N
Q
E
A
D
Q
H
S
A
R
L
F
I
Q
S
Site 84
S691
L
P
R
L
L
S
L
S
N
V
E
E
V
D
T
Site 85
T698
S
N
V
E
E
V
D
T
A
L
Q
N
F
A
S
Site 86
S714
F
C
S
G
M
M
H
S
P
G
F
D
G
N
S
Site 87
S721
S
P
G
F
D
G
N
S
S
L
S
F
Q
M
L
Site 88
S722
P
G
F
D
G
N
S
S
L
S
F
Q
M
L
M
Site 89
S724
F
D
G
N
S
S
L
S
F
Q
M
L
M
N
A
Site 90
S749
L
L
L
N
L
K
L
S
H
G
D
Y
Y
R
K
Site 91
Y753
L
K
L
S
H
G
D
Y
Y
R
K
R
P
T
L
Site 92
Y754
K
L
S
H
G
D
Y
Y
R
K
R
P
T
L
A
Site 93
T759
D
Y
Y
R
K
R
P
T
L
A
P
G
V
M
K
Site 94
Y790
Q
A
W
I
E
E
L
Y
H
Q
V
L
D
R
N
Site 95
S807
G
E
A
G
Y
W
G
S
P
E
D
N
S
L
P
Site 96
S812
W
G
S
P
E
D
N
S
L
P
L
I
T
M
L
Site 97
S842
M
A
S
A
A
T
E
S
P
F
A
Q
S
R
R
Site 98
S853
Q
S
R
R
I
D
D
S
T
V
A
G
V
A
F
Site 99
S877
K
N
L
I
D
T
L
S
T
P
L
T
G
R
M
Site 100
T881
D
T
L
S
T
P
L
T
G
R
M
A
G
S
S
Site 101
S914
E
R
D
A
I
C
M
S
L
D
G
L
R
K
A
Site 102
S960
E
R
E
A
Q
E
P
S
D
A
I
T
Q
V
K
Site 103
T964
Q
E
P
S
D
A
I
T
Q
V
K
L
K
V
E
Site 104
Y1020
H
V
F
R
V
C
E
Y
V
G
T
L
E
H
N
Site 105
T1023
R
V
C
E
Y
V
G
T
L
E
H
N
H
F
S
Site 106
S1030
T
L
E
H
N
H
F
S
D
G
A
S
Q
P
P
Site 107
S1034
N
H
F
S
D
G
A
S
Q
P
P
L
T
I
S
Site 108
T1039
G
A
S
Q
P
P
L
T
I
S
Q
P
Q
K
A
Site 109
S1041
S
Q
P
P
L
T
I
S
Q
P
Q
K
A
T
G
Site 110
S1049
Q
P
Q
K
A
T
G
S
A
G
L
L
G
D
P
Site 111
S1061
G
D
P
E
C
E
G
S
P
P
E
H
S
P
E
Site 112
S1066
E
G
S
P
P
E
H
S
P
E
Q
G
R
S
L
Site 113
S1072
H
S
P
E
Q
G
R
S
L
S
T
A
P
V
V
Site 114
S1074
P
E
Q
G
R
S
L
S
T
A
P
V
V
Q
P
Site 115
S1092
Q
D
L
V
R
E
G
S
R
G
R
A
S
D
F
Site 116
S1097
E
G
S
R
G
R
A
S
D
F
R
G
G
S
L
Site 117
S1103
A
S
D
F
R
G
G
S
L
M
S
G
S
S
A
Site 118
S1106
F
R
G
G
S
L
M
S
G
S
S
A
A
K
V
Site 119
T1116
S
A
A
K
V
V
L
T
L
S
T
Q
A
D
R
Site 120
T1119
K
V
V
L
T
L
S
T
Q
A
D
R
L
F
E
Site 121
T1129
D
R
L
F
E
D
A
T
D
K
L
N
L
M
A
Site 122
S1148
L
Y
Q
L
K
K
A
S
Q
S
Q
L
F
H
S
Site 123
S1150
Q
L
K
K
A
S
Q
S
Q
L
F
H
S
V
T
Site 124
S1155
S
Q
S
Q
L
F
H
S
V
T
D
T
V
D
Y
Site 125
T1157
S
Q
L
F
H
S
V
T
D
T
V
D
Y
S
L
Site 126
Y1162
S
V
T
D
T
V
D
Y
S
L
A
M
P
G
E
Site 127
S1172
A
M
P
G
E
V
K
S
T
Q
D
R
K
S
A
Site 128
T1173
M
P
G
E
V
K
S
T
Q
D
R
K
S
A
L
Site 129
S1178
K
S
T
Q
D
R
K
S
A
L
H
L
F
R
L
Site 130
S1226
C
H
K
E
R
H
V
S
Q
K
A
V
S
F
I
Site 131
T1242
D
I
L
T
E
V
L
T
D
W
N
E
P
P
H
Site 132
S1289
G
E
L
V
E
V
C
S
T
Q
I
Q
S
G
W
Site 133
T1290
E
L
V
E
V
C
S
T
Q
I
Q
S
G
W
R
Site 134
S1301
S
G
W
R
P
L
F
S
A
L
E
T
V
H
G
Site 135
T1305
P
L
F
S
A
L
E
T
V
H
G
G
N
K
S
Site 136
S1312
T
V
H
G
G
N
K
S
E
M
K
E
Y
L
V
Site 137
Y1317
N
K
S
E
M
K
E
Y
L
V
G
D
Y
S
M
Site 138
Y1322
K
E
Y
L
V
G
D
Y
S
M
G
K
G
Q
A
Site 139
S1323
E
Y
L
V
G
D
Y
S
M
G
K
G
Q
A
P
Site 140
S1399
L
D
Y
L
R
R
C
S
Q
L
L
A
K
I
Y
Site 141
Y1406
S
Q
L
L
A
K
I
Y
K
M
P
L
K
P
I
Site 142
S1430
P
R
R
L
Q
E
Q
S
A
S
S
E
D
G
I
Site 143
S1432
R
L
Q
E
Q
S
A
S
S
E
D
G
I
E
S
Site 144
S1439
S
S
E
D
G
I
E
S
V
L
S
D
F
D
D
Site 145
S1442
D
G
I
E
S
V
L
S
D
F
D
D
D
T
G
Site 146
S1466
E
Q
L
T
A
A
V
S
N
C
P
R
Q
H
Q
Site 147
T1476
P
R
Q
H
Q
P
P
T
L
D
L
L
F
E
L
Site 148
T1488
F
E
L
L
R
D
V
T
K
T
P
G
P
G
F
Site 149
S1520
R
R
S
H
K
D
H
S
Y
W
D
M
A
S
A
Site 150
Y1521
R
S
H
K
D
H
S
Y
W
D
M
A
S
A
N
Site 151
S1550
H
I
Q
S
F
L
H
S
D
I
R
Y
E
S
M
Site 152
Y1554
F
L
H
S
D
I
R
Y
E
S
M
I
N
T
M
Site 153
T1560
R
Y
E
S
M
I
N
T
M
L
K
D
L
F
E
Site 154
T1618
L
Q
D
A
F
S
A
T
L
K
P
V
K
D
L
Site 155
S1631
D
L
L
G
C
F
H
S
G
T
E
S
F
S
G
Site 156
T1633
L
G
C
F
H
S
G
T
E
S
F
S
G
E
G
Site 157
S1635
C
F
H
S
G
T
E
S
F
S
G
E
G
C
Q
Site 158
S1649
Q
V
R
V
A
A
P
S
S
S
P
S
A
E
A
Site 159
S1650
V
R
V
A
A
P
S
S
S
P
S
A
E
A
E
Site 160
S1651
R
V
A
A
P
S
S
S
P
S
A
E
A
E
Y
Site 161
S1653
A
A
P
S
S
S
P
S
A
E
A
E
Y
W
R
Site 162
Y1658
S
P
S
A
E
A
E
Y
W
R
I
R
A
M
A
Site 163
T1673
Q
Q
V
F
M
L
D
T
Q
C
S
P
K
T
P
Site 164
T1679
D
T
Q
C
S
P
K
T
P
N
N
F
D
H
A
Site 165
S1708
P
N
G
H
T
K
K
S
V
S
F
R
E
I
V
Site 166
S1710
G
H
T
K
K
S
V
S
F
R
E
I
V
V
S
Site 167
S1741
E
E
F
V
K
G
P
S
P
G
E
E
K
T
I
Site 168
T1747
P
S
P
G
E
E
K
T
I
Q
V
P
E
A
K
Site 169
Y1761
K
L
A
G
F
L
R
Y
I
S
M
Q
N
L
A
Site 170
S1763
A
G
F
L
R
Y
I
S
M
Q
N
L
A
V
I
Site 171
S1777
I
F
D
L
L
L
D
S
Y
R
T
A
R
E
F
Site 172
Y1778
F
D
L
L
L
D
S
Y
R
T
A
R
E
F
D
Site 173
T1780
L
L
L
D
S
Y
R
T
A
R
E
F
D
T
S
Site 174
T1786
R
T
A
R
E
F
D
T
S
P
G
L
K
C
L
Site 175
S1787
T
A
R
E
F
D
T
S
P
G
L
K
C
L
L
Site 176
S1848
L
F
E
D
D
E
R
S
T
D
S
S
Q
Q
C
Site 177
T1849
F
E
D
D
E
R
S
T
D
S
S
Q
Q
C
S
Site 178
S1851
D
D
E
R
S
T
D
S
S
Q
Q
C
S
S
E
Site 179
S1852
D
E
R
S
T
D
S
S
Q
Q
C
S
S
E
D
Site 180
S1856
T
D
S
S
Q
Q
C
S
S
E
D
E
D
I
F
Site 181
S1857
D
S
S
Q
Q
C
S
S
E
D
E
D
I
F
E
Site 182
T1866
D
E
D
I
F
E
E
T
A
Q
V
S
P
P
R
Site 183
S1870
F
E
E
T
A
Q
V
S
P
P
R
G
K
E
K
Site 184
S1888
R
A
R
M
P
L
L
S
V
Q
P
V
S
N
A
Site 185
S1942
D
P
F
F
I
L
P
S
F
Q
S
E
S
S
T
Site 186
S1945
F
I
L
P
S
F
Q
S
E
S
S
T
P
S
T
Site 187
S1947
L
P
S
F
Q
S
E
S
S
T
P
S
T
G
G
Site 188
S1948
P
S
F
Q
S
E
S
S
T
P
S
T
G
G
F
Site 189
T1949
S
F
Q
S
E
S
S
T
P
S
T
G
G
F
S
Site 190
S1951
Q
S
E
S
S
T
P
S
T
G
G
F
S
G
K
Site 191
T1952
S
E
S
S
T
P
S
T
G
G
F
S
G
K
E
Site 192
S1956
T
P
S
T
G
G
F
S
G
K
E
T
P
S
E
Site 193
T1960
G
G
F
S
G
K
E
T
P
S
E
D
D
R
S
Site 194
S1962
F
S
G
K
E
T
P
S
E
D
D
R
S
Q
S
Site 195
S1967
T
P
S
E
D
D
R
S
Q
S
R
E
H
M
G
Site 196
S1969
S
E
D
D
R
S
Q
S
R
E
H
M
G
E
S
Site 197
S1976
S
R
E
H
M
G
E
S
L
S
L
K
A
G
G
Site 198
S1978
E
H
M
G
E
S
L
S
L
K
A
G
G
G
D
Site 199
S1991
G
D
L
L
L
P
P
S
P
K
V
E
K
K
D
Site 200
T2021
Y
T
M
A
A
D
K
T
I
S
K
L
M
T
E
Site 201
T2027
K
T
I
S
K
L
M
T
E
Y
K
K
R
K
Q
Site 202
S2039
R
K
Q
Q
H
N
L
S
A
F
P
K
E
V
K
Site 203
S2061
L
G
P
R
G
Q
D
S
P
L
L
Q
R
P
Q
Site 204
S2079
D
Q
G
Q
M
R
H
S
F
S
A
G
P
E
L
Site 205
S2081
G
Q
M
R
H
S
F
S
A
G
P
E
L
L
R
Site 206
S2095
R
Q
D
K
R
P
R
S
G
S
T
G
S
S
L
Site 207
S2097
D
K
R
P
R
S
G
S
T
G
S
S
L
S
V
Site 208
S2100
P
R
S
G
S
T
G
S
S
L
S
V
S
V
R
Site 209
S2101
R
S
G
S
T
G
S
S
L
S
V
S
V
R
D
Site 210
S2103
G
S
T
G
S
S
L
S
V
S
V
R
D
A
E
Site 211
S2105
T
G
S
S
L
S
V
S
V
R
D
A
E
A
Q
Site 212
T2154
S
Q
L
T
C
H
V
T
D
I
R
V
R
Q
A
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation